Maxwell 16 FFPE Plus LEV DNA Purification Kit from Promega

Product Specs
ItemMaxwell 16 FFPE Plus LEV DNA Purification Kit
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS1135
Quantity48 preps
SourceCell/Tissue, Fixed Tissue
Nucleic Acid TypeGenomic DNA
Applicationspurification

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Integrated Clinical, Molecular and Immunological Characterization of Pulmonary Sarcomatoid Carcinomas Reveals an Immune Escape Mechanism That May Influence Therapeutic StrategiesInternational Journal of Molecular SciencesJune 23, 2023Susann Stephan-Falkenau, Anna Streubel, Thomas Mairinger, Torsten-Gerriet Blum, Jens Kollmeier, Fabian D. Mairinger, Torsten Bauer, Joachim Pfannschmidt, Manuel Hollmann, Michael Wessollyaccording to the manufacturer’s instructions using the Promega Maxwell 16 FFPE PLUS LEV DNA Kit (Cat. #AS1135) or RNA Kit (Cat. #AS1260) on the Maxwell instrument (Promega, Madison, WI, USA). The concentration

(2) The Neo-PLANET phase II trial of neoadjuvant camrelizumab plus concurrent chemoradiotherapy in locally advanced adenocarcinoma of stomach or gastroesophageal junctionNat CommunNovember 10, 2022Zhaoqing Tang, Yan Wang, Dan Liu, Xuefei Wang, Chen Xu, Yiyi Yu, Yuehong Cui, Cheng Tang, Qian Li, Jing Sun, et al.FFPE sections of specimens. Maxwell 16 FFPE Plus LEV DNA Purification Kit (Promega, catalog number AS1135) was used to extract FFPE DNA after FFPE sample sections were scalpeled and deparaffinized. Total DNA

(3) Concurrent loss of MLH1, PMS2 and MSH6 immunoexpression in digestive system cancers indicating a widespread dysregulation in DNA repair processesFrontiers in OncologyOctober 26, 2022Nic Gabriel Reitsam, Bruno MÃ¤rkl, Sebastian Dintner, Johanna Waidhauser, Dmytro Vlasenko, Bianca Grosserstatus DNA was extracted from paraffin-embedded tissue by using the Maxwell® FFPE Plus DNA Kit (Promega, AS1135 [Madison, Wisconsin, USA]) following the manufacturer’s protocol. DNA methylation patterns in the CpG

(4) Global evolution of the tumor microenvironment associated with progression from preinvasive invasive to invasive human lung adenocarcinomaCell ReportsApril 5, 2022Nasser K. Altorki, Alain C. Borczuk, Sebron Harrison, Lauren K. Groner, Bhavneet Bhinder, Vivek Mittal, Olivier Elemento, Timothy E. McGrawvariant calling DNA was extracted with Maxwell 16 FFPE Plus LEV DNA Purification Kit from Promega, Part# AS1135. DNA extracted from tumor adjacent normal lung served as reference genome for each tumor. Less than

(5) Rapid idiosyncratic mechanisms of clinical resistance to KRAS G12C inhibitionJournal of Clinical InvestigationFebruary 15, 2022Yihsuan S. Tsai, Mark G. Woodcock, Salma H. Azam, Leigh B. Thorne, Krishna L. Kanchi, Joel S. Parker, Benjamin G. Vincent, Chad V. Pecot(Promega, AS1030) and from FFPE slides using the Maxwell 16 FFPE Plus LEV DNA Purification Kit (Promega, AS1135) following the manufacturer’s protocols (Promega, TM284 and TM349, respectively). RNA was extracted

(6) Evaluating the efficacy of a priming dose of cyclophosphamide prior to pembrolizumab to treat metastatic triple negative breast cancerJournal for ImmunoTherapy of CancerFebruary 4, 2022Carey K Anders et al.(Promega AS3000). DNA was extracted using the Maxwell 16 FFPE Plus LEV DNA Purification Kit (Promega AS1135) following the manufacturer’s protocols (Promega TM349). RNA was extracted using the Maxwell 16 LEV simplyRNA Tissue Kit

(7) Validation of genetic classifiers derived from mouse and human tumors to identify molecular subtypes of colorectal cancerHuman pathologyJanuary 1, 2022Snow SM, Matkowskyj KA, Maresh M, Clipson L, Vo TN, Johnson KA, Deming DA, Newton MA, Grady WM, Pickhardt PJ, et al.tumors on a Maxwell 16 AS2000 (Promega) using the Maxwell 16 FFPE+LEV DNA Purification Kit (Promega AS1135) according to manufacturer instructions. Isolated DNA was sequenced using the QiaSeq Comprehensive Cancer

(8) Performance of 16S Metagenomic Profiling in Formalin-Fixed Paraffin-Embedded versus Fresh-Frozen Colorectal Cancer TissuesCancers (Basel)October 29, 2021Alessandra Borgognone, Garazi Serna, Marc Noguera-Julian, Lidia Alonso, Mariona Parera, Francesc Català-Moll, Lidia Sanchez, Roberta Fasani, Roger Paredes, Paolo Nuciforoinstructions. DNA from FFPE samples was extracted using the Maxwell 16 FFPE Plus LEV DNA Purification kit (#AS1135, Promega Corporation, Madison, WI, USA) following the manufacturer’s instructions. Extracted DNA was

(9) A novel somatic mutation in GNB2 provides new insights to the pathogenesis of Sturge–Weber syndromeHuman Molecular GeneticsOctober 13, 2021Roar Fjær et al.of Maxwell Incubation Buffer (from Maxwell 16 FFPE Plus LEV DNA Purification Kit, Promega cat. no. AS1135) was inserted in the lid of the collecting tubes (MicroTube 500, Carl Zeiss MicroImaging GmbH, Göttingen,

(10) Clinical Features and Multiplatform Molecular Analysis Assist in Understanding Patient Response to Anti-PD-1/PD-L1 in Renal Cell CarcinomaCancers (Basel)March 23, 2021Eileen Shiuan et al.normal tissue using the Maxwell 16 FFPE Plus LEV DNA Purification Kit (Promega (Madison, WI, USA) #AS1135) and RNA Purification Kit (Promega #AS1260) respectively, on the Promega Maxwell 16 instrument. 2.3.

(11) One byte at a time: evidencing the quality of clinical service next-generation sequencing for germline and somatic variantsEuropean Journal of Human GeneticsFebruary 1, 2020Maria Weronika Gutowska-Ding, Zandra C. Deans, Christophe Roos, Jukka Matilainen, Farrah Khawaja, Kim Brügger, Jo Wook Ahn, Christopher Boustred, Simon J. Patton5 µg of DNA extracted using the Promega Maxwell 16 FFPE plus LEV DNA Purification Kit (Promega cat# AS1135) from a formalin fixed, paraffin embedded (FFPE) cell line product (HD200 containing a multiplex of

(12) microRNA expression in tumour tissue and plasma in patients with newly diagnosed metastatic prostate cancerTumor BiologyMay 19, 2018Ahmed Hussein Zedan, Torben Frøstrup Hansen, Jannie Assenholt, Mindaugas Pleckaitis, Jonna Skov Madsen, Palle Jörn Sloth OstherParaffin was removed from the FFPE tissue samples by the addition of 180 mL incubation buffer (Promega, AS1135) and 20 mL Proteinase K (Promega, AS1460) and incubated for 1 h at 70°C. And 400 mL lysis buffer (Promega,

(13) Atypical pituitary adenoma with MEN1 somatic mutation associated with abnormalities of DNA mismatch repair genes; MLH1 germline mutation and MSH6 somatic mutationEndocrine JournalSeptember 30, 2017Shinsuke Uraki, Hiroyuki Ariyasu, Asako Doi, Hiroto Furuta, Masahiro Nishi, Kokichi Sugano, Naoko Inoshita, Naoyuki Nakao, Shozo Yamada, Takashi AkamizuPromega), QIAamp® DNA Blood Midi Kit (51185; QIAGEN) or Maxwell® 16 FFPE Plus LEV DNA Purification Kit (AS1135; Promega). We performed whole genome sequencing (Illmina, TruSeq Nano DNA Sample Prep Kit) of genomic

(14) The prognostic value of simultaneous tumor and serum RAS/RAF mutations in localized colon cancerCancer MedicineMay 1, 2017Caroline Emilie B. Thomsen, Ane Lindegaard Appelt, Rikke Fredslund Andersen, Jan Lindebjerg, Lars Henrik Jensen, Anders Jakobsenwere purified on the MAXWELLTM 16 LEV instrument using FFPE Plus LEV DNA Purification kit (Promega AS1135) according to the manufacturer's recommendations. DNA was eluted in 50 μL nuclease‐free water and further

(15) TERT promoter mutations and prognosis in solitary fibrous tumorModern PathologyDecember 1, 2016Armita Bahrami, Seungjae Lee, Inga-Marie Schaefer, Jennifer M Boland, Kurt T Patton, Stanley Pounds, Christopher D Fletcherformalinfixed paraffin-embedded sections by using the Maxwell 16 FFPE Plus LEV DNA Purification Kit (AS1135, Promega) according to the manufacturer’s protocol. Mutations in the TERT promoter region from positions

(16) Microsatellite polymorphism in the P1 promoter region of the IGF-1 gene is associated with endometrial cancerMolecular Medicine ReportsJune 1, 2016WOJCIECH KWASNIEWSKI, ANNA GOZDZICKA-JOZEFIAK, MARIA WOLUN-CHOLEWA, GRZEGORZ POLAK, JADWIGA SIEROCINSKA-SAWA, ANNA KWASNIEWSKA, JAN KOTARSKIfor automated DNA isolation with use of the Maxwell 16 FFPE Plus LEV DNA Purification kit (cat. no. AS1135; Promega Corp.). Quantitative analysis of the DNA obtained was performed using a Novaspec II automatic

(17) Analysis of cytosine-adenine repeats in P1 promoter region of IGF-1 gene in peripheral blood cells and cervical tissue samples of females with cervical intraepithelial lesions and squamous cervical cancerMolecular Medicine ReportsFebruary 1, 2015WOJCIECH KWASNIEWSKI, ANNA GOZDZICKA-JOZEFIAK, MARIA KOTARSKA, GRZEGORZ POLAK, BARTLOMIEJ BARCZYNSKI, JUSTYNA BRONIARCZYK, WITOLD NOWAK, MARIA WOLUN-CHOLEWA, ANNA KWASNIEWSKA, JAN KOTARSKIarchival paraffin tissue was carried out using the Maxwell 16 FFPE Plus LEV DNA Purification kit (cat. no. AS1135; Promega, Madison, WI, USA), apparatus for automated DNA isolation, and a computer program for this

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