Gene Pulser®/MicroPulser™ Electroporation Cuvettes, 0.2 cm gap from Bio-Rad

Product Specs
ItemGene Pulser®/MicroPulser™ Electroporation Cuvettes, 0.2 cm gap
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number1652082
ApplicationsCell Counting Methods, Stem Cell Research
TypeElectroporation Cuvettes
Brand/TrademarkLSG CBB

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Cyclic AMP is a critical mediator of intrinsic drug resistance and fatty acid metabolism in M. tuberculosiseLifeFebruary 22, 2023Andrew I Wong, Tiago Beites, Kyle A Planck, Rachael A Fieweger, Kathryn A Eckartt, Shuqi Li, Nicholas C Poulton, Brian C VanderVen, Kyu Y Rhee, Dirk Schnappinger, et al.electrocompetent mycobacteria were mixed and transferred to a 2 mm electroporation cuvette (Bio-Rad #1652082). Where necessary, 100 ng of plasmid plRL19 (Addgene plasmid #163634) was also added. Electroporation

(2) Ligands selectively tune the local and global motions of neurotensin receptor 1 (NTS1)Cell ReportsJanuary 31, 2023Fabian Bumbak, Miquel Pons, Asuka Inoue, Juan Carlos Paniagua, Fei Yan, Hongwei Wu, Scott A. Robson, Ross A.D. Bathgate, Daniel J. Scott, Paul R. Gooley, et al.Eporator,Eppendorf,Cat# 4309000035 Electroporation cuvettes Biorad GenePulser 0.2cm gap,Biorad,Cat# 1652082 Fluorescence activated cell sorter FACSAria III,BD Biosciences,N/A Flow cytometer LSR Fortessa X-20

(3) Application of combined CRISPR screening for genetic and chemical-genetic interaction profiling in Mycobacterium tuberculosisScience AdvancesNovember 25, 2022Mei-Yi Yan, Dandan Zheng, Si-Shang Li, Xin-Yuan Ding, Chun-Liang Wang, Xiao-Peng Guo, Lingjun Zhan, Qi Jin, Jian Yang, Yi-Cheng Suninto 200-μl aliquots, and transferred to a 0.2-cm electrode gap electroporation cuvette (Bio-Rad, #1652082). Cells were electroporated using a Gene Pulser Xcell electroporation system (Bio-Rad, #1652660) at

(4) The ABC transporter MsbA adopts the wide inward-open conformation in E. coli cellsScience AdvancesOctober 14, 2022Laura Galazzo, Gianmarco Meier, Dovile Januliene, Kristian Parey, Dario De Vecchis, Bianca Striednig, Hubert Hilbi, Lars V. SchÃ¤fer, Ilya Kuprov, Arne Moeller, et al.MsbA_E506Q mutant) (fig. S16). The mixture was transferred to cooled 2-mm electroporation cuvettes (Bio-Rad, 1652082) and electroporated at 2.5 kV, 200 ohms, and 25 μF. After electroporation, 1 ml of cooled LB was added

(5) Generation of dual-gRNA library for combinatorial CRISPR screening of synthetic lethal gene pairsSTAR ProtocolsSeptember 16, 2022Shan Tang, Xue Wu, Jinghui Liu, Qiongsi Zhang, Xinyi Wang, Shuai Shao, Birkan Gokbag, Kunjie Fan, Xiaoqi Liu, Fuhai Li, et al.paper,https://github.com/Shantang3/Combinatorial-CRISPR-Screen-Anaylysis-Pipeline ,, Other,Other,Other ,, "Gene Pulser/MicroPulser Electroporation Cuvettes, 0.2 cm gap",Bio-Rad,1652086 "Petri Dishes with Clear Lid, 150 × 15 MM,100/CS",Thermo Fisher Scientific,FB0875714 Cell Culture

(6) LRH ‐1/ NR5A2 interacts with the glucocorticoid receptor to regulate glucocorticoid resistanceEMBO reportsSeptember 5, 2022Svenja Michalek, Thomas Goj, Anna Pia Plazzo, Blerim Marovca, Beat Bornhauser, Thomas Brunner(2017),https://synergyfinder.fimm.fi/synergy/20210603104758451136/ ZEN Software,Carl Zeiss Microscopy,N/A Other,Other,Other "Gene Pulser/MicroPulser Electroporation Cuvettes, 0.2 cm gap",Bio‐Rad Laboratories,Cat# 1652086 LentiCRISPRv2 target guide sequence cloning protocol,Addgene,https://media.addgene.org/cms/filer_public/4f/ab/4fabc269‐56e2‐4ba5‐92bd‐09dc89c1e862/zhang_lenticrisprv2_and_lentiguide_oligo_cloning_protocol_1.pdf 647 EdU Click Proliferation Kit

(7) CRISPRi chemical genetics and comparative genomics identify genes mediating drug potency in Mycobacterium tuberculosisNature MicrobiologyJune 1, 2022Shuqi Li et al.electrocompetent mycobacteria were mixed and transferred to a 2 mm electroporation cuvette (Bio-Rad 1652082). Where necessary, 100 ng plasmid plRL19 (Addgene plasmid 163634) was also added. Electroporation was

(8) The essential M. tuberculosis Clp protease is functionally asymmetric in vivoScience advancesMay 6, 2022d'Andrea FB, Poulton NC, Froom R, Tam K, Campbell EA, Rock JM.with ~100 ng of CRISPRi plasmid DNA and plRL19 before electroporation in a 2-mm cuvette (Bio-Rad, #1652082) using the Gene Pulser X cell electroporation system (Bio-Rad, #1652660) set at 2500 V, 700 ohms, and

(9) Genome-wide gene expression tuning reveals diverse vulnerabilities of M. tuberculosisCellAugust 19, 2021Barbara Bosch et al.per transformation). The cells:DNA mix was transferred to a 2 mm electroporation cuvette (Bio-Rad #1652082) and electroporated at 2500 kV, 700 ohms, and 25 μF. Each transformation was recovered in 5 mL 7H9 media

(10) Population scale nucleic acid delivery to Caenorhabditis elegans via electroporationG3 Genes|Genomes|GeneticsJuly 14, 2021Anastasia S Khodakova, Daniela Vidal Vilchis, Dana Blackburn, Ferdinand Amanor, Buck S Samuelmixed by pipetting, and transferred to 0.2 cm pre-chilled electroporation cuvettes (Biorad, Cat. No. 1652082). Animals were electroporated at 300 V for 10 ms (unless otherwise specified) by square-wave single

(11) RIPiT-Seq: A tandem immunoprecipitation approach to reveal global binding landscape of multisubunit ribonucleoproteinsMethods in EnzymologyJune 29, 2021Zhongxia Yi, Guramrit SinghMagnetic Rack Separator (Sergi Lab Supplies) Gene Pulser Electroporation Cuvettes, 0.2cm gap (Bio-Rad #1652082) or similar products 3.1.1 Cell line HCT 116, Human Colorectal Carcinoma (ATCC CCL-247) 3.1.2 Reagents 5′ DNA Adenylation Kit

(12) Construction and Quantitation of a Selectable Protein Splicing Sensor Using Gibson Assembly and Spot TitersCurrent ProtocolsMarch 19, 2021Daniel Woods, Danielle S. LeSassier, Ikechukwu Egbunam, Christopher W. Lennonequivalent) Culture tubes (VWR 60819–820 or equivalent) Bio-Rad Electroporation Cuvettes, 0.2 cm gap (Bio-Rad # 1652082) Bio-Rad Gene Pulser (Bio-Rad #1652660) Sterile 500 mL baffled Erlenmeyer flask (Millipore Sigma #CLS4450500

(13) Generation of Recombinant SARS‐CoV‐2 Using a Bacterial Artificial ChromosomeCurrent Protocols in MicrobiologyDecember 1, 2020Kevin Chiem, Chengjin Ye, Luis Martinez‐Sobridocat. no. 15400‐054): dilute to 10× in 1× PBS prior to use Gene Pulser Electroporation System (Bio‐Rad)Gene Pulser/MicroPulser Electroporation Cuvettes, 0.2 cm gap (Bio‐Rad, cat. no. 1652086)10‐cm culture tubesIncubator (PHCbi, model cat. no. MCO‐170AICUVDL)MaxQ Large

(14) Virion Display: A High‐Throughput Method to Express Functional Membrane ProteinsCurrent Protocols in Molecular BiologyNovember 5, 2020Guan‐Da Syu, Eric Johansen, Heng ZhuClonase II Enzyme (Thermo Fisher Scientific Catalog #: 11789020) 0.2 cm cuvettes (Bio-Rad, Catalog #: 1652082) DH5α cells (Thermo Fischer Scientific, Catalog #: 18265017) TOP10 cells (Thermo Fischer Scientific,

(15) CRISPR-Cas9-Based Genome Editing and Cytidine Base Editing in Acinetobacter baumanniiSTAR ProtocolsOctober 28, 2020Yu Wang, Zhipeng Wang, Quanjiang JiGene Pulser Xcell™ Electroporation System,Bio-rad,CAT# 165-2660; CAT# 165-2662 2 mm electroporation cuvette,Bio-Rad,CAT# 1652082

(16) Preparation of Yeast tRNA Sample for NMR SpectroscopyBIO-PROTOCOLJune 20, 2020Marjorie Catala, Alexandre Gato, Carine Tisné, Pierre Barraudextraction 1. Gene Pulser®/MicroPulserTM Electroporation Cuvettes 0.2 cm gap (Bio-Rad, catalog number: 1652082) 2. 50 ml conical tube (e.g., FalconTM, product number: 352070) 3. Electrocompetent bacteria Escherichia coli

(17) Group A Streptococcal S Protein Utilizes Red Blood Cells as Immune Camouflage and Is a Critical Determinant for Immune EvasionCell ReportsDecember 3, 2019Igor H. Wierzbicki et al.cells were transformed with 10 μg of pHY304-ess::cat by electroporation using BioRad Gene Pulser II and Gene Pulser/MicroPulser Electroporation Cuvettes, 0.2 cm gap (BioRad) (cooled on ice before application) at the following settings: voltage 1.75 kV, resistance 400

(18) Efficient Construction and Effective Screening of Synthetic Domain Antibody LibrariesMethods and ProtocolsFebruary 14, 2019Arghavan Solemani Zadeh, Alissa Grässer, Heiko Dinter, Maximilian Hermes, Katharina SchindowskiGene Pulser® /MicroPulser™ Electroporation Cuvettes, 0.2 cm gap (Micropulser™, Bio-Rad, Munich, Germany; Cat. no.: 1652082) PEG-8000 (Sigma-Aldrich Chemie GmbH, Munich, Germany; Cat. no.: 729108-1G) Monarch™ DNA Gel Extraction Kit

(19) A Two-Step Approach for the Design and Generation of NanobodiesInternational Journal of Molecular SciencesNovember 2, 2018Hanna J. Wagner, Sarah Wehrle, Etienne Weiss, Marco Cavallari, Wilfried Weberelectroporator (Multiporator, Eppendorf, Hamburg, Germany) with Gene Pulser® electroporation cuvettes (0.2 cm gap; Bio-Rad, #1652082) at 2.5 kV for 5 ms. Transformed bacteria were grown on LB agar supplemented with 1% (w/v) glucose and

(20) Candidate Essential Genes in Burkholderia cenocepacia J2315 Identified by Genome-Wide TraDISFrontiers in MicrobiologyAugust 22, 2016Yee-Chin Wong, Moataz Abd El Ghany, Raeece Naeem, Kok-Wei Lee, Yung-Chie Tan, Arnab Pain, Sheila Nathanice for 30 min. The cell suspension was transferred to an ice-cold electroporation cuvette (Cat. No. 1652082, Bio-Rad; 2-mm-wide gap) and pulsed using the Gene Pulser (Cat. No. 1652660, Bio-Rad) at 2.5 kV, 25

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8/27/2020Electroporation Cuvettes: No Sparking At All! Atul SinghIn comparison to other cuvettes, BIO-RAD cuvettes is better and no sparking till 5ul of ligated reaction or hifi DNA assembly mixture.