QuadroMACS Separator from Miltenyi Biotec

(1) Differential in vivo labeling with barcoded antibodies allows for simultaneous transcriptomic profiling of airway, lung tissue and intravascular immune cellsFrontiers in ImmunologyNovember 29, 2023Barbara C. Mindt, John Kim, Troy Warren, Yang Song, Antonio DiGiandomenico(Beckman Coulter)Hemacytometer (Hausser Scientific, cat# 3100)QuadroMACS™ Separator (Miltenyi Biotec, cat# 130-090-976)MACS MultiStand (Miltenyi Biotec, cat# 130-042-303)LS columns (Miltenyi Biotec, cat# 130-042-401)Microcentrifuge

(2) Development and implementation of natural killer cell simultaneous ADCC and direct killing assayHeliyonNovember 28, 2023Maia M.C. Bennett-Boehm, Anna R. Mahr, Sean T. Hartwell, Alexander K. Regan, Isabelle S. Weber, Arriana Blackmon, Cami R. Bisson, Angela N. Truong, Bella A. Circo, Jaden Nienhueser, et al.mixed (via pipetting), then incubated (4 °C, 10 min). The QuadraMACS magnet (Miltenyi Biotec, order #130-090-976) was assembled with LD columns (Miltenyi Biotec, order #130-042-901) and pre-separation filters (Miltenyi Biotec

(3) M‐CSF directs myeloid and NK cell differentiation to protect from CMV after hematopoietic cell transplantationEMBO Molecular MedicineNovember 8, 2023Prashanth K Kandalla, Julien Subburayalu, Clément Cocita, Bérengère de Laval, Elena Tomasello, Johanna Iacono, Jessica Nitsche, Maria M Canali, Wilfried Cathou, Gilles Bessou, et al.depleted with anti‐CD3 and anti‐CD15 microbeads using a QuadroMACS separator (Miltenyi Biotec, Cat. 130‐090‐976) and LS columns (Miltenyi Biotec, Cat. 130‐042‐401). Cell viability and purity of selection were confirmed

(4) Expanding the MAPPs Assay to Accommodate MHC-II Pan Receptors for Improved Predictability of Potential T Cell EpitopesBiologySeptember 21, 2023Hartman K, Steiner G, Siegel M, Looney CM, Hickling TP, Bray-French K, Springer S, Marban-Doran C, Ducret A.Cat: #130-050-201) using the QuadroMACS system (Miltenyi Biotec, Bergisch, Gladbach, Germany; Cat: #130-090-976) according to the manufacturer’s recommendations. Cells were cultured at a density of 0.3 × 106 cells/mL

(5) Mouse intraductal modeling of primary ductal carcinoma in situSTAR ProtocolsSeptember 1, 2023Stefan J. Hutten, Fariba Behbod, Colinda L.G.J. Scheele, Jos Jonkersdish,Greiner,Cat# 628160 EVO CAM II,Vision Engineering,N/A QuadroMACS separator,Miltenyi Biotec,Cat# 130-090-976 MACS MultiStand,Miltenyi Biotec,Cat# 130-042-303 Hot plate A4,Labotect,Cat# 16481

(6) Rescue of Alzheimer's disease phenotype in a mouse model by transplantation of wild-type hematopoietic stem and progenitor cellsCell reportsAugust 29, 2023Mishra P, Silva A, Sharma J, Nguyen J, Pizzo DP, Hinz D, Sahoo D, Cherqui S.Kit,Biolegend,423101 Anti-Sca1 Microbead Kit,Miltenyi Biotec,130-123-124 Miltenyi QuadroMACS Separator,Miltenyi Biotec,130-090-976 Miltenyi LS columns,Miltenyi Biotec,130-042-401 Miltenyi gentleMACS Octo Dissociator with Heaters,Miltenyi Biotec

(7) Two different isoforms of osteopontin modulate myelination and axonal integrityFASEB bioAdvancesAugust 1, 2023Nilsson G, Mottahedin A, Zelco A, Lauschke VM, Ek CJ, Song J, Ardalan M, Hua S, Zhang X, Mallard C, et al.Column (130‐042‐401, Miltenyi Biotec), which was placed in the magnetic field of a QuadroMACS Separator (130‐090‐976, Miltenyi Biotec). To increase purity, the O4+ cells were applied to a second LS column and the magnetic

(8) Targeting the TCA cycle can ameliorate widespread axonal energy deficiency in neuroinflammatory lesionsNature metabolismAugust 1, 2023Tai YH, Engels D, Locatelli G, Emmanouilidis I, Fecher C, Theodorou D, Müller SA, Licht-Mayer S, Kreutzfeldt M, Wagner I, et al.LS columns (Miltenyi Biotec, 130-042-401) were placed in a magnetic QuadroMACS separator (Miltenyi Biotec, 130-090-976) followed by the equilibration step with 3 ml IPB. The samples were applied to the column in repeating

(9) microRNA-132 regulates gene expression programs involved in microglial homeostasisiScienceJune 16, 2023Hannah Walgrave, Amber Penning, Giorgia Tosoni, Sarah Snoeck, Kristofer Davie, Emma Davis, Leen Wolfs, Annerieke Sierksma, Mayte Mars, Taofeng Bu, et al.into a pre-rinsed LS column (Cat#130-042-401, Miltenyi Biotec) placed in a QuadroMACS Separator (Cat#130-090-976, Miltenyi Biotec). Flowthrough was collected and pooled with flowthough after washing the column with

(10) Lactate promotes neuronal differentiation of SH-SY5Y cells by lactate-responsive gene sets through NDRG3-dependent and -independent mannersThe Journal of biological chemistryJune 1, 2023Xu Y, Kusuyama J, Osana S, Matsuhashi S, Li L, Takada H, Inada H, Nagatomi R.Neuron Isolation Kit, mouse (130-115-389, Miltenyi Biotec), LS Column (130-042-401, Miltenyi Biotec), and MACS Separator (130-090-976, Miltenyi Biotec) according to the manufacturer's protocol. Approximately 53% of cells were isolated

(11) Protocol to generate endothelial cells, pericytes, and fibroblasts in one differentiation round from human-induced pluripotent stem cellsSTAR ProtocolsJune 1, 2023Tea Soon Park, Rishabh Hirday, Amir Ali, Roba Megersa, Rafael Villasmil, Eric Nguyen, Kapil BhartimL,Corning,431118 LS columns,Miltenyi,130-042-401 MidiMACS or QuadroMACS separator,Miltenyi,130-042-302/130-090-976

(12) Angiotensin receptor blockers retard the progression and fibrosis via inhibiting the viability of (AGTR1+) CAFs in intrahepatic cholangiocarcinomaClinical and translational medicineMarch 1, 2023Li JH, Wu X, Ni X, Li YX, Xu L, Hao XY, Zhao W, Zhu XX, Yin XY.FAST buffer at 4°C for 15 min and then applied to LS columns (130‐042‐401) in a QuadroMACS Separator (130‐090‐976). A total of 5–10 passages of primary fibroblasts were used in the experiments. 2.3 Cell culture and

(13) Depletion and activation of microglia impact metabolic connectivity of the mouse brainJournal of neuroinflammationFebruary 24, 2023Gnörich J, Reifschneider A, Wind K, Zatcepin A, Kunte ST, Beumers P, Bartos LM, Wiedemann T, Grosch M, Xiang X, et al.pre-wet LS columns (Miltenyi Biotec, 130-042-401) were placed at QuadroMACS™ Separator (Miltenyi Biotec, 130-090-976). The cell suspensions were applied onto the columns. The columns were washed with 2 × 1 ml PBS-0.5%

(14) Utilization of ethanolamine phosphate phospholyase as a unique astrocytic markerFrontiers in Cellular NeuroscienceJanuary 30, 2023Hiroshi Tsujioka, Toshihide Yamashitatrapped in LS columns (Miltenyi, 130-042-401) under a magnetic field of QuadroMACS Separator (Miltenyi, 130-090-976). Two LS columns were used for each sample for purification. Flow-through (FT) of LS columns (FT fraction)

(15) SOX2 is essential for astrocyte maturation and its deletion leads to hyperactive behavior in miceCell reportsDecember 20, 2022Wang Y, Zhang S, Lan Z, Doan V, Kim B, Liu S, Zhu M, Hull VL, Rihani S, Zhang CL, et al.cells 2x107, max number of total cells 4x107) was placed in the magnetic field of a MACS Separator (Cat#130-090-976, Miltenyi Biotec) and rinsed with 3 mL of 0.5% BSA/PBS buffer. Cell suspension was applied onto the

(16) An optimized protocol for the isolation of rare stromal cell populations from the mouse spleenSTAR protocolsDecember 16, 2022Alexandre YO, Mueller SN.130-042-401 MACS Multistand,Miltenyi Biotec,Cat# 130-042-303 QuadroMACSTM Separator,Miltenyi Biotec,Cat# 130-090-976 Petri dish,Falcon,Cat# 353002 1 mL Syringes Slip Tip,Terumo,Cat# SMSLIP1T Terumo Agani Hypodermic

(17) Isolation, culture, and immunophenotypic analysis of bone marrow HSPCs from patients with myelodysplastic syndromesSTAR ProtocolsDecember 1, 2022Irene Ganan-Gomez, Karen Clise-Dwyer, Simona Collaradius),Eppendorf,Cat#5424R LS Columns,Miltenyi Biotec,Cat#130-042-401 QuadroMACS™ Separator,Miltenyi Biotec,Cat#130-090-976 MACS® MultiStand,Miltenyi Biotec,Cat#130-042-303

(18) Transwell migration assay to interrogate human CAR-T cell chemotaxisSTAR ProtocolsDecember 1, 2022Arman Oner, Sebastian Kobold130-090-544 MACS MultiStand,Miltenyi Biotec,Cat# 130-042-303 QuadroMACS Separator,Miltenyi Biotec,Cat# 130-090-976 xCELLigence RTCA SP System,Agilent,Cat# 00380601030

(19) Lysosomal damage drives mitochondrial proteome remodelling and reprograms macrophage immunometabolismNature communicationsNovember 28, 2022Bussi C, Heunis T, Pellegrino E, Bernard EM, Bah N, Dos Santos MS, Santucci P, Aylan B, Rodgers A, Fearns A, et al.through an LS column (Miltenyi 130-042-401) in the field of a QuadroMACS separator magnet (Miltenyi 130-090-976). The column was washed three times with MACS/BSA, then positively selected cells were eluted, centrifuged,

(20) In Vitro Photodynamic Treatment Modality for A375 Melanoma Cell Line Using a Sulphonated Aluminum Phthalocyanine Chloride-Photosensitizer-Gold Nanoparticle ConjugatePharmaceuticsNovember 16, 2022Mkhobongo B, Chandran R, Abrahamse H.by using a magnetic-activated cell sorting system (MACS), the QuadroMACS Separator (Miltenyi Biotec, 130-090-976, Biocom Africa, Johannesburg, South Africa), and the CD133 (MicroBead Kit, Miltenyi Biotec, 130-100-830,

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(1)

7/27/2022Holds LD and LS Columns Well for Depletion Anita QuallsI use this separator on a MACS multi-stand for column depletion of mouse lymph nodes and splenocytes weekly. These magnets work well, and it is really nice to have four in a row which nicely fit on ... Read Review
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1/9/2019Magnet for Manual Bead-Based Cell SeparationDr. Min Hee OhAs an immunology lab, we perform many antibody bead-based cell separations using the Miltenyi Bio isolation kits. Using these kits requires a magnet since it requires magnetic columns (LS) to enrich ... Read Review
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11/26/2018Separator for CD8 and CD4 Isolation Meng SunOur lab wants a clean CD8+ or CD4+ population that we can use for in-vitro assays. Therefore, we use this magnet for our isolations. It is sturdy and efficient.
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10/19/2018Strong Magnet for Manual Cell Separation Wei Xu.Our lab studies the mTOR signaling pathway in T cell differentiation with an emphasis on both CD4 and CD8 T cells. We routinely conduct T cell experiments to analyze the differentiation process into ... Read Review
(1)

3/14/2018Magnet for Manual Cell Separation Nina FlerinOur lab routinely works with different cell populations isolated from human PBMCs. Since our autoMACS broke we have been using this magnet for all of our cell separations. We have only used it with ... Read Review
(1)

7/6/2015Use of Miltenyi Biotec QuadroMacs Seperation Unit in the negative selection of CD4+ T cells Rebecca WilshusenThe Quadromacs Seperation unit is used to seperate CD4+ T cells from lymph nodes and spleens of immunized mice.

QuadroMACS Separator

QuadroMACS Separator from Miltenyi Biotec

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Miltenyi Biotec

Miltenyi Biotec

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QuadroMACS Separator from Miltenyi Biotec

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