Immobilon® NOW Transfer Membrane rolls for Western blotting from MilliporeSigma

Product Specs
ItemImmobilon® NOW Transfer Membrane rolls for Western blotting
CompanyMilliporeSigma
Price Supplier Page View Company Product Page
Catalog NumberIEVH85R / IPVH85R / IPFL85R / ISEQ85R / IMDISP
QuantityEA
Pore Size0.2 to 45 µm

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MilliporeSigma

400 Summit Drive

Burlington, Massachusetts 01803

United States

Phone: (800)-645-5476
(800)-645-5439

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Website: www.SigmaAldrich.com/

(1) Apoptotic signaling clears engineered Salmonella in an organ-specific mannerElifeDecember 6, 2023Taylor J. Abele, Zachary P. Billman, Lupeng Li, Carissa K. Harvest, Alexia K. Bryan, Gabrielle R Magalski, Joseph P Lopez, Heather N. Larson, Xiao-Ming Yin, Edward A. Miaoprotein. Protein was then transferred onto a 0.45 µm PVDF membrane (Millipore, Burlington, MA, Cat. IPFL85R), blocked with 5% non-fat dried milk in TBS plus 0.01% Tween (TBST) for 1 hr at room temperature, and

(2) N6-methyladenosine of TRIM27 enhances the stem cell-type phenotype of cisplatin-resistant colorectal cancer cellsBiochemistry and Biophysics ReportsDecember 1, 2023Jun-qiong Zheng, Ying Zhan, Wen-jing Huang, Zhi-yong Chen, Wei-hao Wuwas performed to isolate all the proteins and transfer them onto 0.45 μm PVDF membranes (Millipore, IPFL85R). After blocking the PVDF membranes with TBS-T (5 % skim milk), followed by primary and secondary antibodies

(3) 5′-Methylthioadenosine strongly suppresses RANKL-induced osteoclast differentiation and function via inhibition of RANK-NFATc1 signalling pathwaysHeliyonNovember 18, 2023Purithat Rattajak, Aratee Aroonkesorn, Carl Smythe, Rapepun Wititsuwannakul, Thanawat Pitakpornpreechaseparated using SDS-PAGE with 12 % acrylamide gel and electro-transferred to the PVDF membrane (No. IPVH85R, Millipore, Jaffrey, NH, USA). Consequently, membranes were blocked with 5 % non-fat dried milk for

(4) Human tauopathy strains defined by phosphorylation in R1-R2 repeat domains of tauActa Neuropathologica CommunicationsOctober 27, 2023Ethan D. Smith, Quan Vo, Benoit I. Giasson, David R. Borchelt, Stefan Prokop, Paramita Chakrabarty#XP04205) and run for 1.5 h at 100 V. Blots were then transferred to 0.45 μm PVDF membranes (Millipore #IPFL85R) for 2 h at 300mAmps before being blocked in 0.5% Casein for 1 h. Blots were incubated in appropriate

(5) 15-keto-PGE2 alleviates nonalcoholic steatohepatitis through its covalent modification of NF-κB factorsiScienceOctober 20, 2023Siow-Wey Hee, Yi-Cheng Chang, Lynn Su, Ing-Jung Chen, Yung-Ming Jeng, Meng-Lun Hsieh, Yu-Chia Chang, Fu-An Li, Daniel Liao, Shiau-Mei Chen, et al."RIPA Lysis Buffer, 10X",Millipore,20-188 polyvinylidene fluoride (PVDF) microporous membrane,Millipore,IPVH85R Luminata Crescendo Western HRP substrate,Millipore,WBLUR0500 TurboFect Transfection Reagents,Thermo Scientific

(6) KLF12 overcomes anti-PD-1 resistance by reducing galectin-1 in cancer cellsJournal for ImmunoTherapy of CancerAugust 16, 2023Yujia Zheng, Hao Zhang, Chu Xiao, Ziqin Deng, Tao Fan, Bo Zheng, Chunxiang Li, Jie Heseparated using SDS-PAGE and transferred onto polyvinylidene fluoride (PVDF) membranes (Millipore, ISEQ85R). After blocking, primary antibodies against Gal-1 (CST, 12936, 1:1000), KLF12 (Abcam, ab129459, 1:1000),

(7) Marine diterpenoid targets STING palmitoylation in mammalian cellsCommunications chemistryJuly 18, 2023Hsiao WC, Niu GH, Lo CF, Wang JY, Chi YH, Huang WC, Tung CW, Sung PJ, Tsou LK, Zhang MM.protein assay. Proteins were then separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, IPVH85R) and probed for TBK1, phospho-TBK1, IRF3, phospho-IRF3 and α-tubulin. Blots were visualized using SuperSignal™

(8) Crucial role of iron in epigenetic rewriting during adipocyte differentiation mediated by JMJD1A and TET2 activityNucleic Acids ResearchJuly 7, 2023Tomohiro Suzuki, Tetsuro Komatsu, Hiroshi Shibata, Akiko Tanioka, Diana Vargas, Reika Kawabata-Iwakawa, Fumihito Miura, Shinnosuke Masuda, Mayuko Hayashi, Kyoko Tanimura-Inagaki, et al.SDS-polyacrylamide gel (Bio-Rad, 4561096) and transferred onto a polyvinylidene difluoride membrane (Millipore, ISEQ85R) or a nitrocellulose membrane (Bio-Rad, 1620097). Membranes were blocked with 5% skimmed milk or 5%

(9) Cangfu Daotan Wan alleviates polycystic ovary syndrome with phlegm-dampness syndrome via disruption of the PKP3/ERCC1/MAPK axisJournal of ovarian researchJuly 7, 2023Li Y, Wu H, Guo Y, Wei C, Guan L, Ju W, Lian F.separated by polyacrylamide gel electrophoresis and transferred onto polyvinylidene fluoride membranes (IPVH85R, Millipore, Germany). The membrane was blocked with 5% bovine serum albumin and probed overnight at

(10) Galectin-1 promotes angiogenesis and chondrogenesis during antler regenerationCellular & Molecular Biology LettersMay 15, 2023Xunsheng Li, Wanwan Shi, Guanning Wei, Jinpeng Lv, Datao Wang, Baorui Xing, Jue Zhou, Jianwei Zhao, Hongmei Sun(Beyotime, P0013, China), separated using 12% SDS-PAGE gel and transferred to PVDF membranes (Millipore, ISEQ85R, USA). The membranes were incubated with 5% nonfat dry milk for 2 h at RT and then overnight at 4 °C

(11) Elicitation of potent neutralizing antibodies in obese mice by ISA 51-adjuvanted SARS-CoV-2 spike RBD-Fc vaccineApplied Microbiology and BiotechnologyMay 1, 2023Chia-Jung Li, Chung-Lin Jiang, Tai-Ling Chao, Shiau-Yu Lin, Ya-Min Tsai, Chong-Syun Chao, Yu-Ting Su, Chun-Jen Chen, Sui-Yuan Chang, Fu-Jung Lin, et al.40 μg of sample in each lane was transferred to the ImmobilonTM-P PVDF membrane (0.45-μm pore size, IPVH85R, Merck Millipore), which was subsequently blocked with the blocking buffer (5% non-fat milk in PBST).

(12) Dickkopf-1 Acts as a Profibrotic Mediator in Progressive Chronic Kidney DiseaseInternational journal of molecular sciencesApril 21, 2023Hsu YC, Chang CC, Hsieh CC, Huang YT, Shih YH, Chang HC, Chang PJ, Lin CL.gel, and then the separated proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (IEVH85R; Millipore, Burlington, MA, USA). The primary antibodies against human or rat DKK1 (sc-25516; Santa Cruz

(13) Interleukin 26 Induces Macrophage IL-9 Expression in Rheumatoid ArthritisInternational Journal of Molecular SciencesApril 19, 2023Yi-Hsun Wang, Yi-Jen Peng, Feng-Cheng Liu, Gu-Jiun Lin, Shing-Hwa Huang, Huey-Kang Sytwu, Chia-Pi ChengMini-PROTEAN Tetra cell system (BioRad, Hercules, CA, USA) then transferred onto an Immobilon-P Membrane (IPVH85R, Merck Millipore, Burlington, MA, USA) in the same system. Membranes were blocked with TBS-T (T9142,

(14) N-acetylcysteine overcomes NF1 loss-driven resistance to PI3Kα inhibition in breast cancerCell Reports MedicineApril 18, 2023Priska Auf der Maur, Marcel P. Trefny, Zora Baumann, Milica Vulin, Ana Luisa Correia, Maren Diepenbruck, Nicolas Kramer, Katrin Volkmann, Bogdan-Tiberius Preca, Pedro Ramos, et al.#1610374) were separated by SDS-PAGE and later transferred to a PVDF membrane (Immobilon-P, Sigma #IPVH85R) at 90 V for 90 min in tris/glycine buffer (BioRad #1610771) supplemented with 20% methanol. The membrane

(15) SCAP contributes to embryonic angiogenesis by negatively regulating KISS-1 expression in miceCell death & diseaseApril 6, 2023Zheng G, Su Y, Wei L, Yao Y, Wang Y, Luo X, Wang X, Ruan XZ, Li D, Chen Y.(PG111, PG113, YAMEI, Shanghai, China) and electrotransferred onto polyvinylidene difluoride membranes (IEVH85R, Millipore, Burlington, MA, USA). After blocking with 3% Bovine Serum Albumin(9048-46-8, Genview, Florida,

(16) Bioinformatics-integrated screening of systemic sclerosis-specific expressed markers to identify therapeutic targetsFrontiers in ImmunologyMarch 30, 2023Jiahui Jin, Yifan Liu, Qinyu Tang, Xin Yan, Miao Jiang, Xu Zhao, Jie Chen, Caixia Jin, Qingjian Ou, Jingjun ZhaoSangon Biotech, China) was applied to separate the protein, which was then blotted to PVDF membranes (IPFL85R, Merck, Germany). After incubation with the primary antibodies ( Supplementary Table 2 ), the PVDF membranes

(17) Qingre Xingyu recipe exerts inhibiting effects on ulcerative colitis development by inhibiting TNFα/NLRP3/Caspase-1/IL-1β pathway and macrophage M1 polarizationCell Death DiscoveryMarch 8, 2023Liqin Ning, Ningyuan Ye, Bai Ye, Zhiwei Miao, Tingting Cao, Weimin Lu, Danhua Xu, Chang Tan, Yi Xu, Jing Yanpolyacrylamide gel electrophoresis, the protein was electro-blotted to a polyvinylidene fluoride membrane (IPVH85R, Millipore) by the wet transfer method, which subsequently underwent 1-h 5% bovine serum albumin sealing

(18) Tubulin isotype regulation maintains asymmetric requirement for α-tubulin over β-tubulinJournal of Cell BiologyMarch 6, 2023Linnea C. Wethekam, Jeffrey K. MooremAmp per gel for 1 h, or 1.5 h to separate β-tubulins in Fig. 5, H–J. Gels were transferred to PVDF (IPFL85R; Millipore) in NuPAGE transfer buffer (25 mM Bicine, 25 mM Bis-Tris, 1 mM EDTA, pH 7.2) at 0.33 mAmp

(19) Human septins organize as octamer-based filaments and mediate actin-membrane anchoring in cellsThe Journal of cell biologyMarch 6, 2023Martins CS, Taveneau C, Castro-Linares G, Baibakov M, Buzhinsky N, Eroles M, Milanović V, Omi S, Pedelacq JD, Iv F, et al.ECL Rainbow Marker (RPN800E; Cytiva). For the Western blot, the gel, the PVDF Immobilon-PSQ membrane (ISEQ85R; MERCK), filter pads, and filter papers were all incubated in transfer buffer (25 mM Tris, 192 mM glycine

(20) Cardiomyocyte Apoptosis Is Associated with Contractile Dysfunction in Stem Cell Model of MYH7 E848G Hypertrophic CardiomyopathyInternational journal of molecular sciencesMarch 3, 2023Loiben AM, Chien WM, Friedman CE, Chao LS, Weber G, Goldstein A, Sniadecki NJ, Murry CE, Yang KC.(Fisher, BP243-1, Hampton, NH, USA)). Proteins were transferred to Immobilon-P membranes (Millipore, IPVH85R, Burlington, MA, USA) at 120 V for 65 min at 4C in 1× tris-glycine-methanol transfer buffer (25 mM tris

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