Wizard Plus SV Minipreps DNA Purification System from Promega

Product Specs
ItemWizard Plus SV Minipreps DNA Purification System
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberA1330
Timeless than 45 minutes.
Quantity50 preps
Yield1–20µg
SourceBacterial
Nucleic Acid TypePlasmid DNA
Applicationspurification

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Protocol for casein kinase 1γ3 CSNK1G3 gene knockout and recombinant gene expression in cultured HeLa cellsSTAR ProtocolsJune 1, 2023Asako Goto, Kentaro HanadaMini Kit,Favorgen Biotech,Cat#FAPDE 001 Wizard Plus SV Minipreps DNA Purification System,Promega,Cat#A1330 QIAquick PCR Purification Kit,QIAGEN,Cat#28104 PureLink RNA Mini Kit,Ambion,Cat#12183018A Immobilon Western Chemiluminescent HRP Substrate

(2) Kisspeptin-10 Mitigates α-Synuclein-Mediated Mitochondrial Apoptosis in SH-SY5Y-Derived Neurons via a Kisspeptin Receptor-Independent MannerInternational Journal of Molecular SciencesMarch 23, 2023Christopher Simon, Tomoko Soga, Ishwar Parharwith the Wizard® Plus SV Minipreps DNA Purification System Kit (Promega, Madison, WI, USA; Cat. #: A1330) in due time. 4.3. Cell Culture The SH-SY5Y neuroblastoma cell line procured from ATCC (Manassas,

(3) Integrin αvβ3 Is a Master Regulator of Resistance to TKI-Induced Ferroptosis in HER2-Positive Breast CancerCancersFebruary 14, 2023Nagpal A, Needham K, Lane DJR, Ayton S, Redvers RP, John M, Selistre-de-Araujo HS, Denoyer D, Pouliot N.extracted from bacterial pellets using Wizard®Plus SV Minipreps DNA Purification System kit (Promega, #A1330) as per the manufacturer’s instruction. For transfection, total plasmid DNA (2.5 μg) containing empty

(4) Assembly of plant virus agroinfectious clones using biological material or DNA synthesisSTAR ProtocolsDecember 1, 2022Fabio PasinT4 DNA ligase (400 U/μL),New England Biolabs,M0202S Wizard® Plus SV minipreps DNA purification system,Promega,A1330 2-(N-Morpholino)ethanesulfonic acid hydrate (MES),Sigma-Aldrich,M8250 "3′,5′-Dimethoxy-4-hydroxyacetophenon

(5) Structure and function of the N-terminal extension of the formin INF2Cellular and Molecular Life SciencesNovember 1, 2022Leticia Labat-de-Hoz, Laura Comas, Armando Rubio-Ramos, Javier Casares-Arias, Laura FernÃ¡ndez-MartÃn, David Pantoja-Uceda, M. Teresa MartÃn, Leonor Kremer, M. Angeles JimÃ©nez, Isabel Correas, et al.Mutagenesis Kit,Stratagene,Cat#200523 Wizard® Plus SV Minipreps DNA Purification Systems,Promega,Cat#A1330 Deposited data,Deposited data,Deposited data Chemical shifts of INF2 2-19 in 30% trifluoroethanol,This

(6) Pesticide residue exposure provides different responses of the microbiomes of distinct cultures of the stored product pest mite Acarus siroBMC MicrobiologyOctober 19, 2022Jan Hubert, Blanka Navratilova, Bruno Sopko, Marta Nesvorna, Thomas W. Phillips°C. The plasmid was then purified with a Wizard® Plus SV Minipreps DNA purification system (Cat. No. A1330, Promega) according to the manufacturer’s protocol. Plasmids were linearized by SacI restriction (Cat.

(7) In silico analyses of leptin and leptin receptor of spotted snakehead Channa punctataPLOS ONEJuly 7, 2022Amrita Bakshi, Umesh Raibacterial culture using commercially available kit, Wizard® Plus SV Minipreps DNA Purification System (#A1330, Promega Corporation, Madison, USA), was subjected to DNA-insert verification by conducting PCR using

(8) Kisspeptin-10 Rescues Cholinergic Differentiated SHSY-5Y Cells from α-Synuclein-Induced Toxicity In VitroInternational Journal of Molecular SciencesMay 6, 2022Christopher Simon, Tomoko Soga, Nafees Ahemad, Saatheeyavaane Bhuvanendran, Ishwar Parharpurified with a Wizard Plus SV Minipreps DNA Purification System (Promega, Madison, WI, USA; Cat. #: A1330). 4.3. Cell Culture The human neuroblastoma SH-SY5Y cell line purchased from ATCC (Manassas, VA, USA;

(9) Semi-Quantitative Assay to Measure Urease Activity by Urinary Catheter-Associated UropathogensFrontiers in Cellular and Infection MicrobiologyMarch 22, 2022Jesus M. Duran Ramirez, Jana Gomez, Chloe L. P. Obernuefemann, Nathaniel C. Gualberto, Jennifer N. WalkerHUC strains encode urease, genomic DNA was extracted using the Promega Miniprep Kit (Promega; cat # A1330) according to the manufacturer’s guidelines, with the exception that 2 ug/ml of Lysostaphin (Sigma;

(10) Effective chimeric antigen receptor T cells against SARS-CoV-2iScienceNovember 19, 2021Guo X, Kazanova A, Thurmond S, Saragovi HU, Rudd CE.Wizard® SV Gel and PCR Clean-Up System,Promega,Cat. #: A9281 Wizard® Plus SV Minipreps DNA Purification Systems,Promega,Cat. #: A1330 Q5 Site-Directed Mutagenesis Kit,New England Biolabs,Cat. #: E0554S NucleoBond® Xtra Maxi EF Kit,TAKARA,Cat.

(11) A phenotypically supervised single-cell analysis protocol to study within-cell-type heterogeneity of cultured mammalian cellsSTAR ProtocolsJune 18, 2021Kevin Chen, Kivilcim Ozturk, Ted Liefeld, Michael Reich, Jill P. Mesirov, Hannah Carter, Stephanie I. Fraley(https://www.promega.com/products/nucleic-acid-extraction/plasmid-purification/wizard-plus-sv-minipreps-dna-purification-systems/?catNum=A1330#protocols)8.Verify the sequence through sequencing with the custom primers. Production of lentiviruses Timing:

(12) Effect of locally isolated phosphate-solubilizing Pseudomonas aeruginosa IBP26 on the growth parameters of chickpea seedlingJournal of the Institute of Science and TechnologyJune 1, 2021Muhammed Emin COGENDER, Nazlı Pınar ARSLAN, Mehmet Nuri AYDOĞANthe manufacturer, the plasmid was purified using Wizard® Plus SV Minipreps DNA Purification System (Promega, A1330) kit and sequenced in Macrogen Company (Netherlands). The 16S rRNA obtained was compared with the other

(13) Generation of TCRÎ±-transduced T cells for adoptive transfer therapy of salmonellosis in miceSTAR ProtocolsMarch 22, 2021Anastasiia Kalinina, Alexandra Bruter, Ludmila Nesterenko, Ludmila Khromykh, Dmitry KazanskyKit,QIAGEN,Cat#28704 QIAGEN Plasmid Midi Kit,QIAGEN,Cat#12143 Wizard Plus SV Minipreps DNA Purification System,Promega,Cat#A1330 1× RBC lysis buffer,Invitrogen,Cat#00-4333 ,, Experimental models: cell lines,Experimental models:

(14) Manipulation of the human tRNA pool reveals distinct tRNA sets that act in cellular proliferation or cell cycle arresteLifeDecember 24, 2020Noa Aharon-Hefetz, Idan Frumkin, Yoav Mayshar, Orna Dahan, Yitzhak Pilpel, Roni Rakfind recombinant plasmid, plasmids were purified Wizard Plus SV Minipreps DNA Purification (Promega; A1330) from ampicillin resistant colonies and subjected to Sanger sequencing. Primer used for the validation

(15) Production, Purification and Characterization of Recombinant Biotinylated Phytochrome B for Extracellular OptogeneticsBIO-PROTOCOLMarch 5, 2020Maximilian Hörner, O. Sascha Yousefi, Wolfgang W. A. Schamel, Wilfried Weberplasmid from E. coli with a miniprep kit, e.g., Wizard Plus SV Miniprep Kit, Promega, catalog number: A1330), store purified plasmid DNA at -20 °C. For plasmid sequence, see Table S1.IceCuvettes for photometer

(16) Genome-wide de novo L1 Retrotransposition Connects Endonuclease Activity with ReplicationCellMay 1, 2019Diane A. Flasch, Ángela Macia, Laura Sánchez, Mats Ljungman, Sara R. Heras, José L. García-Pérez, Thomas E. Wilson, John V. MoranKit for Conventional PCR,Sigma,MP 0025-1KT Wizard Plus SV Minipreps DNA Purification Systems,Promega,A1330 Deposited Data,Deposited Data,Deposited Data PacBio CCS-fastq files (except HeLa) and PA-1 Bru-Seq

(17) Induction of the acid inducible lipF promoter is reversibly inhibited in pH ranges of pH 4.2-4.0BMC Research NotesDecember 1, 2018Rachelle Torres, Donna Dorriz, Beatrice Saviolautilized for selecting resistant plasmids. Plasmids were isolated using Wizard minipreps (Promega, PR-A1330). 50 ng of purified plasmids were transformed via electroporation into M. smegmatis and selection occurred

(18) TOR-autophagy branch signaling via Imp1 dictates plant-microbe biotrophic interface longevityPLOS GeneticsNovember 21, 2018Guangchao Sun, Christian Elowsky, Gang Li, Richard A. Wilsonculture of the transformed JM-109 strain by a Wizard Plus SV Minipreps DNA Purification System (Promega, A1330) and sequenced by Eurofins Genomics. Targeted gene replacement WT protoplasts were generated and transformed

(19) DivergentHemogengenes of teleosts and mammals share conserved roles in erythropoiesis: Analysis using transgenic and mutant zebrafishBiology OpenAugust 28, 2018Michael J. Peters, Sandra K. Parker, Jeffrey Grim, Corey A. H. Allard, Jonah Levin, H. William Detrichwere id entified by blue/white screening and purified with the Wizard Plus SV Miniprep Kit (Promega; A1330) and inserts were sequenced by GENEWIZ, Inc. (Cambridge, USA). Bioinformatic comparison of vertebrate

(20) Non-dispersive phloem-protein bodies (NPBs) of Populus trichocarpa consist of a SEOR protein and do not respond to cell wounding and Ca²⁺PeerJApril 17, 2018Daniel L. Mullendore, Timothy Ross-Elliott, Yan Liu, Hanjo H. Hellmann, Eric H. Roalson, Winfried S. Peters, Michael Knoblauchincubated overnight with the Promega SV Wizard Miniprep kit according to manufacturer’s instructions (#A1330 Promega, Madison, WI, USA), and the gene was sequenced using the flanking M13F: 5′-GTAAAACGACGGCCAGT-3′

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(1)

1/22/2021DNA Purification System for Fast Plasmid Extraction Neha MittalThe Minipreps DNA purification system provides high quality Plasmid DNA even with small amount of bacterial culture. For sequencing good quality of Plasmid DNA is highly required but sometimes ... Read Review
(1)

1/18/2019Good and Useful Kit for Plasmid Extraction for Transfection Wei Yang SitThe transformed bacteria is grown and subsequently collected for plasmid extraction using this kit. The eluted plasmid is then used for transient transfection of Lentiviral production. The kit is easy... Read Review

Wizard® Plus SV Minipreps DNA Purification System From Promega
Promega’s Wizard® Plus SV Minipreps DNA Purification System is a simple and reliable method for ... Read More