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PCR Master Mixes
GoTaq Green Master Mix
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GoTaq Green Master Mix
GoTaq Green Master Mix
GoTaq Green Master Mix from Promega
(6)
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Citations:
(226)
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GoTaq Green Master Mix
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Description
New formulation of Taq DNA Polymerase with enhanced performance over standard Taq DNA Polymerase. Available in two all-in-one master mixes: green and colorless. The green master mix allows direct gel analysis after amplification
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
GoTaq Green Master Mix
Company
Promega
Price
Supplier Page
View Company Product Page
Catalog Number
M7122
Quantity
100 reactions
Concentration
2X
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Promega
2800 Woods Hollow Rd.
Madison
,
Wisconsin
United States
Phone
: 800-356-9526
Fax: 800-356-1970
Company Profile
Website:
http://www.promega.com
Citations (226)
(1)
DMC1 stabilizes crossovers at high and low temperatures during wheat meiosis
Front Plant Sci
August 8, 2023
Tracie N. Draeger, María-Dolores Rey, Sadiye Hayta, Mark Smedley, Azahara C. Martin, Graham Moore
5′-TCAATCCCT CCTTCAAATTACGC-3′ were used.
PCR amplification
was performed using GoTaq® Master Mix (
Promega
,
M7122
), with the following conditions: 3 min 94 °C, 40 cycles of 30 s at 94 °C, 15 s at 58 °C, 1 min at 72
(2)
CRISPR-Cas9 correction of a nonsense mutation in LCA5 rescues lebercilin expression and localization in human retinal organoids
Molecular Therapy - Methods & Clinical Development
June 8, 2023
Tess A.V. Afanasyeva, Dimitra Athanasiou, Pedro R.L. Perdigao, Kae R. Whiting, Lonneke Duijkers, Galuh D.N. Astuti, Jean Bennett, Alejandro Garanto, Jacqueline van der Spuy, Ronald Roepman,
et al.
5′-TCAACCATGCAACACAGTGAAGCT-3′ (reverse).
PCR reactions
were carried out with 100 ng genomic DNA in
GoTaq Green Master Mix
(
M7122
,
Promega
) according to the manufacturer’s instructions.
PCR
thermocycling scheme was as follows: 5 min
(3)
Combined lineage tracing and scRNA-seq reveals unexpected first heart field predominance of human iPSC differentiation
eLife
June 7, 2023
Francisco Xavier Galdos, Carissa Lee, Soah Lee, Sharon Paige, William Goodyer, Sidra Xu, Tahmina Samad, Gabriela V Escobar, Adrija Darsha, Aimee Beck,
et al.
construct to be integrated. PCRs were conducted using the GoTaq Master Mix (
Promega Corporation
Cat.
M7122
), and products were run on a 1% agarose gel in 1 X TAE Buffer.
PCR
primer sequences are found in Figure
(4)
The Occurrence of Folate Biosynthesis Genes in Lactic Acid Bacteria from Different Sources
Food Technology and Biotechnology
June 1, 2023
Fenny Amilia Mahara, Lilis Nuraida, Hanifah Nuryani Lioe, Siti Nurjanah
detection
PCR
amplifications were performed in 20 µL volumes with 10 µL Promega Go Taq Green Master Mix 1× (
M7122
;
Promega Corporation
), containing Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers, 1 µL of 0.5
(5)
An unusual tandem kinase fusion protein confers leaf rust resistance in wheat
Nature Genetics
June 1, 2023
Yajun Wang, Michael Abrouk, Spyridon Gourdoupis, Dal-Hoe Koo, Miroslava Karafiátová, István Molnár, Kateřina Holušová, Jaroslav Doležel, Naveenkumar Athiyannan, Emile Cavalet-Giorsa,
et al.
8).
PCR
amplifications were done in 20 μl reactions containing 1×
GoTaq Green Master Mix
(
Promega
,
M7122
), ∼100 ng genomic DNA and 100 nM primers. Primer pairs K1-F/K1-R, K2-F2/K2-R1 and
vWA
-F2/
vWA
-R1 were
(6)
Establishment and inheritance of minichromosomes from Arabidopsis haploid induction
Chromosoma
June 1, 2023
Ek Han Tan, Benny Ordoñez, Tejas Thondehaalmath, Danelle K. Seymour, Julin N. Maloof, Ravi Maruthachalam, Luca Comai
μL reaction volumes and recommended primer pair concentrations using GoTaq Green Mastermix (
Promega
M7122
) and 55 °C annealing temperatures for 40 cycles. After
PCR
, a 5 μL digestion mix containing 0.1 μL restriction
(7)
Multi-omics analysis of a Bacteroides fragilis isolate from an ulcerative colitis patient defines genetic determinants of fitness in bile
bioRxiv
May 16, 2023
Aretha Fiebig, Matt Schnizlein, Selymar Pena-Rivera, Florian Trigodet, Abhishek Anil Dubey, Miette Hennessy, Anindita Basu, Sebastian Pott, Sushila Dalal, David Rubin,
et al.
clones
were mapped using a two-step nested arbitrary
PCR
approach using 2X GoTaq master mix (
Promega
M7122
). The first reaction (1X GoTaq master mix, 0.3 uM U1 fw, 0.3 uM M13-N7 and 0.5 ul saved culture in a
(8)
Molecular Evaluation of the Impact of Polymorphic Variants in Apoptotic (Bcl-2/Bax) and Proinflammatory Cytokine (TNF-α/IL-8) Genes on the Susceptibility and Progression of Myeloproliferative Neoplasms: A Case-Control Biomarker Study
Current Issues in Molecular Biology
May 2, 2023
Mamdoh S. Moawadh, Rashid Mir, Faris J. Tayeb, Orooba Asim, Mohammad Fahad Ullah
25 pmol each primer, and 10 μL of
GoTaq® Green Master Mix
from
Promega
, Fitchburg, WI, USA (cat no
M7122
). The reaction mixture also received 2 μL of DNA from either the patient or control sample, and nuclease-free
(9)
Genetic profile of thymic epithelial tumors in the Japanese population: an exploratory study examining potential therapeutic targets
Translational Lung Cancer Research
April 28, 2023
Midori Shimada, Hirokazu Taniguchi, Hiroyuki Yamaguchi, Hiroshi Gyotoku, Daisuke Sasaki, Norihito Kaku, Chikako Senju, Hiroaki Senju, Erika Imamura, Shinnosuke Takemoto,
et al.
using Primer3 Input (ELIXIREstonia, Tartu, Estonia). The primers,
GoTaq® Green Master Mix
(Catalog #
M7122
,
Promega
, Madison, WI, USA), water, and each DNA sample were thoroughly mixed, and subsequent
amplification
(10)
eYGFPuv-Assisted Transgenic Selection in Populus deltoides WV94 and Multiplex Genome Editing in Protoplasts of P. trichocarpa × P. deltoides Clone ‘52-225’
Plants (Basel)
April 14, 2023
Guoliang Yuan, Yang Liu, Tao Yao, Wellington Muchero, Jin-Gui Chen, Gerald A. Tuskan, Xiaohan Yang
Transgenic plants
were
PCR
genotyped using GoTaq® Master Mixes (
Promega
, Madison, WI, USA, Cat. No.
M7122
). We used 1 μL of genomic DNA as a template with the following
PCR
cycling conditions: 95 °C for 2 min,
(11)
Mir125b-2 imprinted in human but not mouse brain regulates hippocampal function and circuit in mice
Communications Biology
March 14, 2023
Ming-Yi Chou, Xuhui Cao, Kuan-Chu Hou, Meng-Han Tsai, Chih-Yu Lee, Meng-Fai Kuo, Vin-Cent Wu, Hsin-Yi Huang, Schahram Akbarian, Sheng-Kai Chang,
et al.
(primer sequences are listed in Supplementary Data 5) and
Taq polymerase
(GoTaq® Master Mixes (
Promega
,
M7122
); or AptaTaq™ Fast DNA Polymerase (Roche, 6879110001) for some of the samples), in
C1000 Touch
Thermal
(12)
Multiplex CRISPR-Cas9 Gene-Editing Can Deliver Potato Cultivars with Reduced Browning and Acrylamide
Plants (Basel)
January 13, 2023
Diem Nguyen Phuoc Ly, Sadia Iqbal, John Fosu-Nyarko, Stephen Milroy, Michael G. K. Jones
protocol. The PCRs were conducted with the
GoTaq® Green Master Mix
(
Promega Corporation
, catalogue no.
M7122
) and used 300 ng of cDNA or 100 ng of DNA for each
potato
cultivar in separate 20 µL reactions. The
(13)
The cytokine receptor CRLF3 is a human neuroprotective EV-3 (Epo) receptor
Frontiers in molecular neuroscience
January 1, 2023
Knorr DY, Rodriguez Polo I, Pies HS, Schwedhelm-Domeyer N, Pauls S, Behr R, Heinrich R.
using specific primers as stated below (Table 1).
PCR
was run using
GoTaq Green Master Mix
(
Promega
; #
M7122
) and
PCR
program was set as shown in Table 2.
PCR
products were loaded on a 1% agarose gel and run for
(14)
A standalone incompatible insect technique enables mosquito suppression in the urban subtropics
Communications Biology
December 27, 2022
Qin Zeng, Lingzhi She, Hao Yuan, Yuying Luo, Renke Wang, Wei Mao, Weifeng Wang, Yueting She, Chaojun Wang, Mengyi Shi,
et al.
and ND5 gene
amplification
on a
Bio-Rad
T100 thermal cycler
. Using
GoTaq Green Master Mix
(
Promega
M7122
), mitochondrial COI gene was amplified with specific primers LCO1490 and HCO219836 as follows: initial
(15)
Metabolite Profiling of Gardenia jasminoides Ellis In Vitro Cultures with Different Levels of Differentiation
Molecules
December 14, 2022
Gergana Krasteva, Strahil Berkov, Atanas Pavlov, Vasil Georgiev
were selected for this study (Table S4). For
PCR amplification
,
GoTaq Green Master Mix
(
Promega
, cat.#
M7122
) was used according to the kit’s manual. The reaction mixture (25 µL) consisted of 12.5 µL
GoTaq Green Master Mix
(16)
Vegf signaling between Müller glia and vascular endothelial cells is regulated by immune cells and stimulates retina regeneration
Proceedings of the National Academy of Sciences
December 13, 2022
Soumitra Mitra, Sulochana Devi, Mi-Sun Lee, Jonathan Jui, Aresh Sahu, Daniel Goldman
by
RT-PCR
using gene-specific primers (SI Appendix, Table S1), and
GoTaq Green Master Mix
(
Promega
M7122
) or Radiant 2× RED Taq mix (Alkali Scientific C225) in a 25-μL reaction volume.
PCR
products (7 μL)
(17)
Loss of Pex1 in Inner Ear Hair Cells Contributes to Cochlear Synaptopathy and Hearing Loss
Cells
December 9, 2022
Mauriac SA, Peineau T, Zuberi A, Lutz C, Géléoc GSG.
Genotyping
Genotyping
was performed by
Polymerase Chain Reaction (PCR
) using GoTaq® Master Mix (
Promega
#
M7122
) using primer sets included in Table 1. For the Pex1fl/fl
genotyping
, we followed the protocol designed
(18)
Identification of transient intermediates during spliceosome activation by single molecule fluorescence microscopy
Proceedings of the National Academy of Sciences
November 29, 2022
Xingyang Fu, Harpreet Kaur, Margaret L. Rodgers, Eric J. Montemayor, Samuel E. Butcher, Aaron A. Hoskins
The DNA template was produced from a
PCR
reaction of pBS117 plasmid (49) using Taq DNA polymerase (
M7122
,
Promega
), followed by gel
purification
of the products with a
Wizard SV Gel and PCR Clean-Up System
(19)
Interleukin-38 promoter variants and risk of COVID-19 among Iraqis
Immunobiology
November 9, 2022
Ibtihal A. Al-Karaawi, Wasan W. Al-Bassam, Haneen M. Ismaeel, Ali H. Ad'hiah
(total volume: 20 µL) consisted of 10 µL Master Mix (
GoTaq Green Master Mix
,
Promega
, USA, Cat. No.
M7122
), 1 µL forward primer, 1 µL reverse primer, 2 µL DNA and 6 µL nuclease free water. The thermal cycler
(20)
Association of Polymorphism of the Methyl Tetrahydrofolate Reductase (MTHFR) Gene with Anti-Seizure Medication Response in Pediatric Patients in Jeddah, Saudi Arabia
Medicina (Kaunas)
November 3, 2022
Reem Alyoubi, Abdullah Althomali, Rania Magadmi, Hala S. Abdel kawy, Hadiah Bassam Al Mahdi, Fatemah O. Kamel, Duaa M. Bakhshwin, Maha Jamal, Mohammed Alsieni
patients and controls was performed using the Veriti™ 96-Well Thermal Cycler,
GoTaq Green Master Mix
(Cat#
M7122
,
Promega Corporation
, Madison, WI, USA), and the following primers: F: 5′-TCCCTGTGGTCTCTTCATCC-3′; R:
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Reviews
Write a Review
(1)
7/17/2020
Efficient Amplification of DNA Using GoTaq® Green Master Mix
Xiaoguang Li
I recommend this because it saves a lot of time, and works well. I didn't recycle the gel product.
(1)
7/23/2019
Promega's PCR Master Mix for Colony PCR Works Well
Atul Singh
Best kit I have used so far for colony PCR.
(1)
1/28/2019
Very Easy and Efficient Taq Mastermix
Virginia Folgado
I needed a cheap and efficient PCR mix since I have to do a lot of PCR from bacteria colonies to check presence/absence of my target insert in the plasmid. I found this kit very easy to use; I didn't ...
Read Review
(1)
8/15/2017
Great PCR Master Mix
Chris C
We use this PCR master mix for genotyping PCR reaction for transgenic mice. We selected this product because of its ease of use, just add primers, water and template. It works well for our PCR ...
Read Review
(1)
12/29/2016
Super Easy PCR Premix
Zeji Du
GoTaq Green Master Mix is a premixed ready-to-use solution containing Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by ...
Read Review
(1)
10/14/2015
Premixed and Ready-To-Use Solution for DNA Synthesis
Sunita Meena
GoTaq® Green Master Mix was mixed with primers which are specific for the gene of interest present in cDNA and kept in PCR machine for amplification. The PCR product was directly visualized in ...
Read Review
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GoTaq Green Master Mix from Promega
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