GoTaq Green Master Mix from Promega

Product Specs
ItemGoTaq Green Master Mix
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM7122
Quantity100 reactions
Concentration2X

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) DMC1 stabilizes crossovers at high and low temperatures during wheat meiosisFront Plant SciAugust 8, 2023Tracie N. Draeger, María-Dolores Rey, Sadiye Hayta, Mark Smedley, Azahara C. Martin, Graham Moore5′-TCAATCCCT CCTTCAAATTACGC-3′ were used. PCR amplification was performed using GoTaq® Master Mix (Promega, M7122), with the following conditions: 3 min 94 °C, 40 cycles of 30 s at 94 °C, 15 s at 58 °C, 1 min at 72

(2) CRISPR-Cas9 correction of a nonsense mutation in LCA5 rescues lebercilin expression and localization in human retinal organoidsMolecular Therapy - Methods & Clinical DevelopmentJune 8, 2023Tess A.V. Afanasyeva, Dimitra Athanasiou, Pedro R.L. Perdigao, Kae R. Whiting, Lonneke Duijkers, Galuh D.N. Astuti, Jean Bennett, Alejandro Garanto, Jacqueline van der Spuy, Ronald Roepman, et al.5′-TCAACCATGCAACACAGTGAAGCT-3′ (reverse). PCR reactions were carried out with 100 ng genomic DNA in GoTaq Green Master Mix (M7122, Promega) according to the manufacturer’s instructions. PCR thermocycling scheme was as follows: 5 min

(3) Combined lineage tracing and scRNA-seq reveals unexpected first heart field predominance of human iPSC differentiationeLifeJune 7, 2023Francisco Xavier Galdos, Carissa Lee, Soah Lee, Sharon Paige, William Goodyer, Sidra Xu, Tahmina Samad, Gabriela V Escobar, Adrija Darsha, Aimee Beck, et al.construct to be integrated. PCRs were conducted using the GoTaq Master Mix (Promega Corporation Cat. M7122), and products were run on a 1% agarose gel in 1 X TAE Buffer. PCR primer sequences are found in Figure

(4) An unusual tandem kinase fusion protein confers leaf rust resistance in wheatNature GeneticsJune 1, 2023Yajun Wang, Michael Abrouk, Spyridon Gourdoupis, Dal-Hoe Koo, Miroslava Karafiátová, István Molnár, Kateřina Holušová, Jaroslav Doležel, Naveenkumar Athiyannan, Emile Cavalet-Giorsa, et al.8). PCR amplifications were done in 20 μl reactions containing 1× GoTaq Green Master Mix (Promega, M7122), ∼100 ng genomic DNA and 100 nM primers. Primer pairs K1-F/K1-R, K2-F2/K2-R1 and vWA-F2/vWA-R1 were

(5) Establishment and inheritance of minichromosomes from Arabidopsis haploid inductionChromosomaJune 1, 2023Ek Han Tan, Benny Ordoñez, Tejas Thondehaalmath, Danelle K. Seymour, Julin N. Maloof, Ravi Maruthachalam, Luca ComaiμL reaction volumes and recommended primer pair concentrations using GoTaq Green Mastermix (Promega M7122) and 55 °C annealing temperatures for 40 cycles. After PCR, a 5 μL digestion mix containing 0.1 μL restriction

(6) The Occurrence of Folate Biosynthesis Genes in Lactic Acid Bacteria from Different SourcesFood Technology and BiotechnologyJune 1, 2023Fenny Amilia Mahara, Lilis Nuraida, Hanifah Nuryani Lioe, Siti Nurjanahdetection PCR amplifications were performed in 20 µL volumes with 10 µL Promega Go Taq Green Master Mix 1× (M7122; Promega Corporation), containing Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers, 1 µL of 0.5

(7) Multi-omics analysis of a Bacteroides fragilis isolate from an ulcerative colitis patient defines genetic determinants of fitness in bilebioRxivMay 16, 2023Aretha Fiebig, Matt Schnizlein, Selymar Pena-Rivera, Florian Trigodet, Abhishek Anil Dubey, Miette Hennessy, Anindita Basu, Sebastian Pott, Sushila Dalal, David Rubin, et al.clones were mapped using a two-step nested arbitrary PCR approach using 2X GoTaq master mix (Promega M7122). The first reaction (1X GoTaq master mix, 0.3 uM U1 fw, 0.3 uM M13-N7 and 0.5 ul saved culture in a

(8) Molecular Evaluation of the Impact of Polymorphic Variants in Apoptotic (Bcl-2/Bax) and Proinflammatory Cytokine (TNF-α/IL-8) Genes on the Susceptibility and Progression of Myeloproliferative Neoplasms: A Case-Control Biomarker StudyCurrent Issues in Molecular BiologyMay 2, 2023Mamdoh S. Moawadh, Rashid Mir, Faris J. Tayeb, Orooba Asim, Mohammad Fahad Ullah25 pmol each primer, and 10 μL of GoTaq® Green Master Mix from Promega, Fitchburg, WI, USA (cat no M7122). The reaction mixture also received 2 μL of DNA from either the patient or control sample, and nuclease-free

(9) Genetic profile of thymic epithelial tumors in the Japanese population: an exploratory study examining potential therapeutic targetsTranslational Lung Cancer ResearchApril 28, 2023Midori Shimada, Hirokazu Taniguchi, Hiroyuki Yamaguchi, Hiroshi Gyotoku, Daisuke Sasaki, Norihito Kaku, Chikako Senju, Hiroaki Senju, Erika Imamura, Shinnosuke Takemoto, et al.using Primer3 Input (ELIXIREstonia, Tartu, Estonia). The primers, GoTaq® Green Master Mix (Catalog # M7122, Promega, Madison, WI, USA), water, and each DNA sample were thoroughly mixed, and subsequent amplification

(10) eYGFPuv-Assisted Transgenic Selection in Populus deltoides WV94 and Multiplex Genome Editing in Protoplasts of P. trichocarpa × P. deltoides Clone ‘52-225’Plants (Basel)April 14, 2023Guoliang Yuan, Yang Liu, Tao Yao, Wellington Muchero, Jin-Gui Chen, Gerald A. Tuskan, Xiaohan YangTransgenic plants were PCR genotyped using GoTaq® Master Mixes (Promega, Madison, WI, USA, Cat. No. M7122). We used 1 μL of genomic DNA as a template with the following PCR cycling conditions: 95 °C for 2 min,

(11) Mir125b-2 imprinted in human but not mouse brain regulates hippocampal function and circuit in miceCommunications biologyMarch 14, 2023Chou MY, Cao X, Hou KC, Tsai MH, Lee CY, Kuo MF, Wu VC, Huang HY, Akbarian S, Chang SK, et al.(primer sequences are listed in Supplementary Data 5) and Taq polymerase (GoTaq® Master Mixes (Promega, M7122); or AptaTaq™ Fast DNA Polymerase (Roche, 6879110001) for some of the samples), in C1000 Touch Thermal

(12) Multiplex CRISPR-Cas9 Gene-Editing Can Deliver Potato Cultivars with Reduced Browning and AcrylamidePlants (Basel)January 13, 2023Diem Nguyen Phuoc Ly, Sadia Iqbal, John Fosu-Nyarko, Stephen Milroy, Michael G. K. Jonesprotocol. The PCRs were conducted with the GoTaq® Green Master Mix (Promega Corporation, catalogue no. M7122) and used 300 ng of cDNA or 100 ng of DNA for each potato cultivar in separate 20 µL reactions. The

(13) The cytokine receptor CRLF3 is a human neuroprotective EV-3 (Epo) receptorFrontiers in molecular neuroscienceJanuary 1, 2023Knorr DY, Rodriguez Polo I, Pies HS, Schwedhelm-Domeyer N, Pauls S, Behr R, Heinrich R.using specific primers as stated below (Table 1). PCR was run using GoTaq Green Master Mix (Promega; #M7122) and PCR program was set as shown in Table 2. PCR products were loaded on a 1% agarose gel and run for

(14) A standalone incompatible insect technique enables mosquito suppression in the urban subtropicsCommunications BiologyDecember 27, 2022Qin Zeng, Lingzhi She, Hao Yuan, Yuying Luo, Renke Wang, Wei Mao, Weifeng Wang, Yueting She, Chaojun Wang, Mengyi Shi, et al.and ND5 gene amplification on a Bio-Rad T100 thermal cycler. Using GoTaq Green Master Mix (Promega M7122), mitochondrial COI gene was amplified with specific primers LCO1490 and HCO219836 as follows: initial

(15) Metabolite Profiling of Gardenia jasminoides Ellis In Vitro Cultures with Different Levels of DifferentiationMoleculesDecember 14, 2022Gergana Krasteva, Strahil Berkov, Atanas Pavlov, Vasil Georgievwere selected for this study (Table S4). For PCR amplification, GoTaq Green Master Mix (Promega, cat.# M7122) was used according to the kit’s manual. The reaction mixture (25 µL) consisted of 12.5 µL GoTaq Green Master Mix

(16) Vegf signaling between Müller glia and vascular endothelial cells is regulated by immune cells and stimulates retina regenerationProceedings of the National Academy of SciencesDecember 13, 2022Soumitra Mitra, Sulochana Devi, Mi-Sun Lee, Jonathan Jui, Aresh Sahu, Daniel Goldmanby RT-PCR using gene-specific primers (SI Appendix, Table S1), and GoTaq Green Master Mix (Promega M7122) or Radiant 2× RED Taq mix (Alkali Scientific C225) in a 25-μL reaction volume. PCR products (7 μL)

(17) Loss of Pex1 in Inner Ear Hair Cells Contributes to Cochlear Synaptopathy and Hearing LossCellsDecember 9, 2022Mauriac SA, Peineau T, Zuberi A, Lutz C, Géléoc GSG.Genotyping Genotyping was performed by Polymerase Chain Reaction (PCR) using GoTaq® Master Mix (Promega #M7122) using primer sets included in Table 1. For the Pex1fl/fl genotyping, we followed the protocol designed

(18) Identification of transient intermediates during spliceosome activation by single molecule fluorescence microscopyProceedings of the National Academy of SciencesNovember 29, 2022Xingyang Fu, Harpreet Kaur, Margaret L. Rodgers, Eric J. Montemayor, Samuel E. Butcher, Aaron A. HoskinsThe DNA template was produced from a PCR reaction of pBS117 plasmid (49) using Taq DNA polymerase (M7122, Promega), followed by gel purification of the products with a Wizard SV Gel and PCR Clean-Up System

(19) Interleukin-38 promoter variants and risk of COVID-19 among IraqisImmunobiologyNovember 9, 2022Ibtihal A. Al-Karaawi, Wasan W. Al-Bassam, Haneen M. Ismaeel, Ali H. Ad'hiah(total volume: 20 µL) consisted of 10 µL Master Mix (GoTaq Green Master Mix, Promega, USA, Cat. No. M7122), 1 µL forward primer, 1 µL reverse primer, 2 µL DNA and 6 µL nuclease free water. The thermal cycler

(20) Association of Polymorphism of the Methyl Tetrahydrofolate Reductase (MTHFR) Gene with Anti-Seizure Medication Response in Pediatric Patients in Jeddah, Saudi ArabiaMedicina (Kaunas)November 3, 2022Reem Alyoubi, Abdullah Althomali, Rania Magadmi, Hala S. Abdel kawy, Hadiah Bassam Al Mahdi, Fatemah O. Kamel, Duaa M. Bakhshwin, Maha Jamal, Mohammed Alsienipatients and controls was performed using the Veriti™ 96-Well Thermal Cycler, GoTaq Green Master Mix (Cat# M7122, Promega Corporation, Madison, WI, USA), and the following primers: F: 5′-TCCCTGTGGTCTCTTCATCC-3′; R:

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(1)

7/17/2020Efficient Amplification of DNA Using GoTaq® Green Master Mix Xiaoguang LiI recommend this because it saves a lot of time, and works well. I didn't recycle the gel product.
(1)

7/23/2019Promega's PCR Master Mix for Colony PCR Works Well Atul SinghBest kit I have used so far for colony PCR.
(1)

1/28/2019Very Easy and Efficient Taq Mastermix Virginia FolgadoI needed a cheap and efficient PCR mix since I have to do a lot of PCR from bacteria colonies to check presence/absence of my target insert in the plasmid. I found this kit very easy to use; I didn't ... Read Review
(1)

8/15/2017Great PCR Master Mix Chris C We use this PCR master mix for genotyping PCR reaction for transgenic mice. We selected this product because of its ease of use, just add primers, water and template. It works well for our PCR ... Read Review
(1)

12/29/2016Super Easy PCR Premix Zeji DuGoTaq Green Master Mix is a premixed ready-to-use solution containing Taq DNA polymerase, dNTPs, MgCl2 and reaction buffers at optimal concentrations for efficient amplification of DNA templates by ... Read Review
(1)

10/14/2015Premixed and Ready-To-Use Solution for DNA Synthesis Sunita MeenaGoTaq® Green Master Mix was mixed with primers which are specific for the gene of interest present in cDNA and kept in PCR machine for amplification. The PCR product was directly visualized in ... Read Review