OneTaq2X Master Mix with Standard Buffer from New England Biolabs

Product Specs
ItemOneTaq2X Master Mix with Standard Buffer
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0482S
Concentration2 X
Quantity100 reactions
ApplicationsDNA Analysis
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeMaster Mix
Temperature-20 C

Specifications/Features

Information:
Source: An E. coli strain that carries the Taq DNA Polymerase gene from Thermus aquaticus YT-1 and an E. coli strain that carries the Deep Vent_R DNA Polymerase gene from Pyrococcus species GB-D.

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Dataset on estimate of intra-specific genetic variability of African yam bean (Sphenostylis stenocarpa (Hochst. ex A. Rich.) Harms.) based on rbcL gene markerData in BriefApril 1, 2023Jacob Olagbenro Popoola, Davelyne Ifechukude Eruemulor, Omena Bernard Ojuederie, Abiodun Sunday Oyelakinthe composition as follows: 12.5 µL of NEB OneTaq 2X Master Mix with Standard Buffer (Catalogue No. M0482S), 2 µL of genomic DNA (10–30 ng/µl), 0.5 µL RbcL 1F (5′-ATGTCACCACAAACAGAAAC-3′) and 724R (5-GTAAAATCAAGTCCACCGCG-3)

(2) Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschiiNature CommunicationsDecember 1, 2022Guifang Lin et al.lr42_1F/R (Supplementary Data 8) for 28 cycles. The OneTaq 2x Master Mix (New England Biolabs, USA, Cat.# M0482S) was used in the RT-PCR. The thermocycling conditions were initial denature at 94 °C for 2 min, 25 or

(3) Constitutive Musashi1 expression impairs mouse postnatal development and intestinal homeostasisMolecular Biology of the CellJanuary 1, 2021Thelma T. Chiremba, Kristi L. Neufeld5′-CCCAAGGCACACAAAAAACC-3′. PCR conditions, using OneTaq DNA polymerase (New England BioLabs [NEB], #M0482S), were 95°C for 5 min, 35 cycles (95°C for 30 s, 60°C for 30 s, and 72°C for 1 min) and 72°C for 10

(4) Accuracy of a Smartphone-Based Object Detection Model, PlantVillage Nuru, in Identifying the Foliar Symptoms of the Viral Diseases of Cassava–CMD and CBSDFrontiers in Plant ScienceDecember 18, 2020Latifa M. Mrisho, Neema A. Mbilinyi, Mathias Ndalahwa, Amanda M. Ramcharan, Annalyse K. Kehs, Peter C. McCloskey, Harun Murithi, David P. Hughes, James P. Leggnucleic acid was added to the PCR master mix containing One Taq 2x Master mix with standard buffer (M0482S, New England Biolabs) and 200 nM each of the forward and reverse primers. A negative control (no-template)

(5) Co‐evolving wing spots and mating displays are genetically separable traits in DrosophilaEvolutionJune 1, 2020Jonathan H. Massey, Gavin R. Rice, Anggun S. Firdaus, Chi‐Yang Chen, Shu‐Dan Yeh, David L. Stern, Patricia J. Wittkoppligated to each sample of tagmented gDNA with 14 cycles of PCR using OneTaq 2x Master Mix (NEB, cat# M0482S), and all samples were pooled into a single multiplexed sequencing library. Agencourt AMPure XP beads

(6) Sequential Interferon β-Cisplatin Treatment Enhances the Surface Exposure of Calreticulin in Cancer Cells via an Interferon Regulatory Factor 1-Dependent MannerBiomoleculesApril 21, 2020Yang PM, Hsieh YY, Du JL, Yen SC, Hung CF.purchased from Geneaid Biotech (New Taipei City, Taiwan). The OneTaq 2X Master Mix with Standard Buffer (M0482S) was purchased from New England Biolabs (Beverly, MA, USA). 2.2. Cell Culture The human cervical adenocarcinoma

(7) Programmable CRISPR interference for gene silencing using Cas13a in mosquitoesJournal of GenomicsMarch 1, 2020Aditi Kulkarni, Wanqin Yu, Alex S. Moon, Ashmita Pandey, Kathryn A. Hanley, Jiannong XucDNA as template, 0.2 μM primers (primer sequences are presented in Table S1) and 2 × PCR Master mix (M0482S, NEB), with the following cycling parameters: 35 cycles of denaturing at 95°C for 15 seconds, annealing

(8) Probiotic Bacillus subtilis Protects against α-Synuclein Aggregation in C. elegansCell ReportsJanuary 14, 2020María Eugenia Goya, Feng Xue, Cristina Sampedro-Torres-Quevedo, Sofia Arnaouteli, Lourdes Riquelme-Dominguez, Andrés Romanowski, Jack Brydon, Kathryn L. Ball, Nicola R. Stanley-Wall, Maria DoitsidouAssays,Critical Commercial Assays ,, OneTaq® 2X Master Mix with Standard Buffer,New England Biolabs (NEB),Cat# M0482S GoTaq® G2 Green Master Mix,Promega,Cat#M782A QIAquick PCR Purification Kit,QIAGEN,Cat#28104 Qubit

(9) The Arabidopsis Transcriptome Responds Specifically and Dynamically to High Light StressCell ReportsDecember 17, 2019Jianyan Huang, Xiaobo Zhao, Joanne Chory"Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase",Thermo,K1671 iTaq Universal SYBR® Green Supermix,Bio-rad,1725121 OneTaq® 2 × Master Mix with Standard Buffer,NEB,M0482S Deposited Data,Deposited Data,Deposited Data Raw RNA-Seq data from Arabidopsis,This paper,GEO: GSE111062 Experimental Models

(10) A Novel Dual Lentiviral CRISPR-based Transcriptional Activation System for Gene Expression Regulation in NeuronsBIO-PROTOCOLSeptember 5, 2019Katherine E Savell, Faraz A Sultan, Jeremy J Day(Qiagen, catalog number: 27106) 21. OneTaq 2x master mix with standard buffer (NEB, catalog number: M0482S) 22. PureLink HiPure Plasmid Filter Midiprep Kit (Fisher, catalog number: K210015) 23. PureLink HiPure Plasmid Filter Maxiprep Kit

(11) Daun02 inactivation of behaviourally-activated Fos-expressing neuronal ensemblesCurrent Protocols in NeuroscienceJuly 2, 2016Eisuke Koya, Gabriella Margetts‐Smith, Bruce T. Hopehydrochloride solution 1M (Tris-HCl) pH 8 (Cat no., T2694–1L, Sigma) OneTaq DNA polymerase mastermix (Cat no. M0482S, New England Biolabs) Oligos (IDT DNA Technologies, standard synthesis, and desalt prepration) PCR primers

(12) Screening of Selected Soil and Endophytic Fungi for Lovostatin Biosynthetic Genes lovE and lovFJournal of Microbial & Biochemical TechnologyJanuary 1, 2015Bhargavi SD, Praveen VK, Savitha Jtemplate for PCR amplification using the designed primers. One Taq2X Master Mix with standard buffer (M0482S-NEB, England) was used for PCR amplification. This master mix was used at 1X concentration with DNA template

Write a Review

(1)

3/20/2018PCR Mastermix for Conventional PCR Alex RayWe have several mouse colonies which require ongoing genotyping. When we first started doing in-house genotyping, we had issues with different PCR master mixes. The OneTaq PCR master mix works very ... Read Review