Cell Lysis Buffer (10X) from Cell Signaling Technology

Product Specs
ItemCell Lysis Buffer (10X)
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number9803S
Quantity15 ml
Research AreaRelated Products
ApplicationsELISA

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) The synthetic oleanane triterpenoid CDDO-2P-Im binds GRP78/BiP to induce unfolded protein response-mediated apoptosis in myelomaMolecular oncologyDecember 1, 2023Luo G, Aldridge K, Chen T, Aslot V, Kim BG, Han EH, Singh N, Li S, Xiao TS, Sporn MB, et al.sacrificed 12 h later for analyses of tumors. Tumors were lysed for protein using Cell Lysis solution (#9803; Cell Signaling, Danvers, MA, USA) and subsequently prepared for immunoblot. Female and Male NSG (NOD

(2) Fanconi anemia associated protein 20 (FAAP20) plays an essential role in homology-directed repair of DNA double-strand breaksCommun BiolAugust 24, 2023Anna Palovcak, Fenghua Yuan, Ramiro Verdun, Liang Luo, Yanbin Zhangrotated at 4 °C for 2 h. Beads were washed 5× using a magnetic stand and 1× wash buffer (Cell Signaling #9803). Bound protein was eluted by boiling for 5 min. Immunoprecipitated protein samples and 2% Input samples

(3) CD44 acts as a coreceptor for cell-specific enhancement of signaling and regulatory T cell induction by TGM1, a parasite TGF-β mimicProceedings of the National Academy of Sciences of the United States of AmericaAugust 22, 2023van Dinther M, Cunningham KT, Singh SP, White MPJ, Campion T, Ciancia C, van Veelen PA, de Ru AH, González-Prieto R, Mukundan A, et al.(Fresenius Kabi) and the cells were harvested in 1× Cell Lysis Buffer (Cell Signaling Technologies, 9803). After spinning, the supernatant was incubated with Neutravidin beads (Pierce, 29201) for 1 h at 4

(4) Maintenance of Flap Endonucleases for Long-Patch Base Excision DNA Repair in Mouse Muscle and Neuronal Cells Differentiated In VitroInternational journal of molecular sciencesAugust 12, 2023Caston RA, Fortini P, Chen K, Bauer J, Dogliotti E, Yin YW, Demple B.lysed using a buffer with sodium dodecyl sulfate (SDS) (Cell Signaling Technology, Danvers, MA, USA, #9803; following the supplier’s protocol), followed by centrifugation at 12,000× g for 10 min, and the supernatants

(5) The Activation of the LIMK/Cofilin Signaling Pathway via Extracellular Matrix-Integrin Interactions Is Critical for the Generation of Mature and Vascularized Cardiac OrganoidsCellsAugust 9, 2023Ji-Min Noh, Seung-Cheol Choi, Myeong-Hwa Song, Kyung Seob Kim, Seongmin Jun, Jae Hyoung Park, Ju Hyeon Kim, Kyoungmi Kim, Tae Hee Ko, Jong-Il Choi, et al.Germany). 2.4. Western Blotting The hESC-COs were washed twice with PBS and lysed with a 1× cell lysis buffer (9803, Cell Signaling Technology, Danvers, MA, USA) plus 1 mM of phenylmethylsulfonyl fluoride (P7626, Sigma–Aldrich).

(6) Graphene Sensor Arrays for Rapid and Accurate Detection of Pancreatic Cancer Exosomes in Patients’ Blood Plasma SamplesACS NanoAugust 8, 2023Tianyi Yin, Lizhou Xu, Bruno Gil, Nabeel Merali, Maria S. Sokolikova, David C. A. Gaboriau, Daniel S. K. Liu, Ahmad Nizamuddin Muhammad Mustafa, Sarah Alodan, Michael Chen, et al.4 (Vivaspin Turbo 4, 10 kDa, PES, Sartorius) at 4000g for 2 h at 4 °C. Cell lysis RIPA Buffer (No. 9803, Cell Signaling Technology) and additive PMSF (200 mM, Cell Signaling Technology) were used. The EVs

(7) Myonectin protects against skeletal muscle dysfunction in male mice through activation of AMPK/PGC1α pathwayNat CommunAugust 4, 2023Yuta Ozaki, Koji Ohashi, Naoya Otaka, Hiroshi Kawanishi, Tomonobu Takikawa, Lixin Fang, Kunihiko Takahara, Minako Tatsumi, Sohta Ishihama, Mikito Takefuji, et al.Western blot analysis Skeletal muscle and C2C12 cell samples were solubilized with lysis buffer (Cell Signaling Technology, Cat 9803 S) and protease inhibitor cocktail (Roche, Cat 11697498001). The protein concentration was determined

(8) Salt-inducible kinase inhibition promotes the adipocyte thermogenic program and adipose tissue browningMolecular metabolismAugust 1, 2023Shi F, de Fatima Silva F, Liu D, Patel HU, Xu J, Zhang W, Türk C, Krüger M, Collins S.and then treated with 1 μM Iso for 6 h on the following day, and lysed in 1× Cell Lysis Buffer (CST, #9803) supplemented with 1× protease inhibitor and 1× phosphatase inhibitor cocktail. For Western blot analysis

(9) LIN28B promotes cell invasion and colorectal cancer metastasis via CLDN1 and NOTCH3JCI InsightJuly 24, 2023Kensuke Sugiura, Yasunori Masuike, Kensuke Suzuki, Alice E. Shin, Nozomu Sakai, Hisahiro Matsubara, Masayuki Ohtsuka, Peter A. Sims, Christopher J. Lengner, Anil K. Rustgi3. Western blot. Protein samples were extracted using Cell Lysis Buffer (Cell Signaling Technology, 9803) containing 1 mM phenylmethylsulfonyl fluoride and a protease inhibitor cocktail (MilliporeSigma, P8340).

(10) Development of a human iPSC-derived placental barrier-on-chip modeliScienceJuly 21, 2023Agathe Lermant, Gwenaëlle Rabussier, Henriëtte L. Lanz, Lindsay Davidson, Iain M. Porter, Colin E. MurdochPowerUp SYBR Green Master Mix,ThermoFisher,Cat#A25742 Cell Lysis Buffer (10X),Cell Signaling Technology,Cat#9803 DC™ Protein Assay Kit II,Bio-rad,Cat#5000112 ,, Deposited data,Deposited data,Deposited data ,, RNAseq

(11) Bruton’s tyrosine kinase inhibition reduces disease severity in a model of secondary progressive autoimmune demyelinationActa Neuropathologica CommunicationsJuly 12, 2023Kirsten Scarlett Evonuk, Sen Wang, Josh Mattie, C. J. Cracchiolo, Reine Mager, Željko Ferenčić, Ethan Sprague, Brandon Carrier, Kai Schofield, Evelyn Martinez, et al.analysis, lysis buffer was prepared from 10 × Cell Lysis Buffer (Cell Signaling Technology, cat. no. 9803S) and 100 × Halt Protease Inhibitor Cocktail (ThermoFisher Scientific, cat. no. 78429). Left brain hemispheres

(12) Response of the Glutathione (GSH) Antioxidant Defense System to Oxidative Injury in Necrotizing EnterocolitisAntioxidants (Basel)July 5, 2023Alena Golubkova, Tyler Leiva, Katherine Snyder, Camille Schlegel, Sarah M. Bonvicino, Martin-Paul Agbaga, Richard S. Brush, Jason M. Hansen, Peter F. Vitiello, Catherine J. Huntercultures. Protein extraction was completed using a RIPA lysis cocktail (1×, Cell Signaling Technology, #9803, Danvers, MA, USA) with phosphatase and proteinase inhibitors (Phosphatase Inhibitor Cocktail 2, Sigma Aldrich

(13) H3K27me3-modulated Hofbauer cell BMP2 signalling enhancement compensates for shallow trophoblast invasion in preeclampsiaEBioMedicineJuly 1, 2023Deng J, Zhao HJ, Zhong Y, Hu C, Meng J, Wang C, Lan X, Wang X, Chen ZJ, Yan J, et al.analysis. Immunoblotting Cells and placentas were lysed in ice-cold cell lysis buffer (Cell Signaling Technology, 9803) and RIPA lysis buffer (Beyotime, P0013B), respectively, with added protease/phosphatase inhibitor cocktail

(14) Nuclear transport maintenance of USP22-AR by Importin-7 promotes breast cancer progressionCell Death DiscoveryJuly 1, 2023Geng-Xi Cai, Wei-Yao Kong, Yuan Liu, Shu-Yi Zhong, Qing Liu, Yuan-Fei Deng, Guo-Lin Yeassays Protein samples were collected at low temperatures using the cell lysis buffer (Cell Lysis Buffer (#9803) (Cell Signaling Technology, Beverly, MA, USA), protease inhibitor mixture, phosphatase inhibitor mixture,

(15) Terfezia boudieri and Terfezia claveryi inhibit the LPS/IFN-γ-mediated inflammation in RAW 264.7 macrophages through an Nrf2-independent mechanismScientific ReportsJune 21, 2023Abdelhameed S. Dawood, Mohamed S. Sedeek, Mohamed A. Farag, Anwar Abdelnaserwere purchased from Synbio Technologies (Monmouth Junction, NJ, USA). Cell Lysis Buffer (10X; Cat. No. 9803S), Protease Inhibitor Cocktail (100X; Cat. No. 5871), and Prestained Protein Marker, Broad Range (11–190 kDa

(16) Atlantic Salmon Gill Epithelial Cell Line (ASG-10) as a Suitable Model for Xenobiotic BiotransformationMetabolitesJune 20, 2023Lada Ivanova, Christiane Kruse Fæste, Anita SolhaugMycoAlert® Mycoplasma detection kit was purchased from Lonza (Basel, Switzerland), while cell lysis buffer (#9803), nonfat dry milk and horseradish peroxidase (HRP)-conjugated anti-mouse antibody were obtained from

(17) Discovery of senolytics using machine learningNature communicationsJune 10, 2023Smer-Barreto V, Quintanilla A, Elliott RJR, Dawson JC, Sun J, Campa VM, Lorente-Macías Á, Unciti-Broceta A, Carragher NO, Acosta JC, et al.GCGTTTGGAGTGGTAGAAATCT. Western blot analysis Cell lysates were prepared using Cell Lysis Buffer (Cell Signalling 9803S). Clear lysates were quantified by Bradford colorimetric assay. Samples were resolved by polyacrylamide gel electrophoresis

(18) Iodine-Biofortified Lettuce Can Promote Mitochondrial Dependent Pathway of Apoptosis in Human Gastrointestinal Cancer CellsInternational Journal of Molecular SciencesJune 7, 2023Olga Sularz, Aneta Koronowicz, Sylwester Smoleń, Cayla Boycott, Barbara StefanskaMA, USA). 4.6. Protein Expression Analysis The cell lysates were obtained using Cell Lysis Buffer (#9803) and Cell Fractionation Kit (#9038) with the addition of Protease/Phosphatase Inhibitor Cocktail (#5872)

(19) A widely used pan-isoform-FBXW7 antibody used in cell cycle studies does not detect FBXW7Cell CycleJune 3, 2023Hannah Ennis, Denise M McDonaldT75 flasks were washed with 5 ml ice-cold PBS. Ice-cold 1× CST lysis buffer (1 ml; Cell Signaling, 9803) supplemented with 1mM PMSF (Merck, 93482), 5mM Sodium fluoride (NaF, Merck, 67414) and EDTA-free protease

(20) The COPS3-FOXO3 positive feedback loop regulates autophagy to promote cisplatin resistance in osteosarcomaAutophagyJune 1, 2023Niu J, Yan T, Guo W, Wang W, Ren T, Huang Y, Zhao Z, Yu Y, Chen C, Huang Q, et al.phosphate-buffered saline (PBS; Meilunbio, MA0015) and lysed with cell lysis buffer (Cell Signaling Technology, 9803) containing protease and phosphatase inhibitors (Thermo Fisher Scientific, 78440). Total protein concentration

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10/30/2015Cell Lysis Buffer That Works PENG SHIThis cell lysis buffer is used to lyse cells under nondenaturing conditions. The lysis effect in relatively mild and we use it together with other reagents and could get satisfactory total protein ... Read Review

Cell Lysis Buffer (10X) from Cell Signaling Technology

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Cell Lysis Buffer (10X) from Cell Signaling Technology

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