QuantiLum Recombinant Luciferase from Promega

Product Specs
ItemQuantiLum Recombinant Luciferase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1701
Quantity1mg
Concentration10–15mg/ml

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Structural basis of impaired disaggregase function in the oxidation-sensitive SKD3 mutant causing 3-methylglutaconic aciduriaNature CommunicationsApril 11, 2023Sukyeong Lee, Sang Bum Lee, Nuri Sung, Wendy W. Xu, Changsoo Chang, Hyun-Eui Kim, Andre Catic, Francis T. F. Tsaiabsence of protein. Protein disaggregation assay Recombinant firefly luciferase (FFL; 50 µM) (Cat. #E1701, Promega) was denatured using 8 M urea in refolding buffer (25 mM HEPES-KOH pH 8.0, 150 mM potassium

(2) Differential contributions of the proteasome, autophagy, and chaperones to the clearance of arsenite-induced protein aggregates in yeastJournal of Biological ChemistryDecember 1, 2022Sansan Hua, Agnieszka Kłosowska, Joana I. Rodrigues, Gabriel Petelski, Lidia A. Esquembre, Emma Lorentzon, Lars F. Olsen, Krzysztof Liberek, Markus J. Tamáskinase was purchased from Sigma–Aldrich (10127566001). Untagged Luciferase was purchased from Promega (E1701). cfSGFP gene (45) was synthesized and cloned by Genescript to pET3a plasmid and expressed in the Escherichia

(3) Complementary protocols to evaluate inhibitors against the DnaK chaperone networkSTAR ProtocolsJune 17, 2022Aweon Richards, Gideon K. Yawson, Brock Nelson, Tania J. Lupoli10735078001 Telaprevir,Cayman Chemical,CAS: 402957-28-2 Firefly Luciferase: QuantiLum Recombinant Luciferase,Promega,Cat # E1701 ATP (≥99% HPLC),Sigma-Aldrich,CAS: 24369-07-8 ADP (Ultrapure),VMR Life Science,CAS: 16178-48-6 Hemoglobin

(4) Class-specific interactions between Sis1 J-domain protein and Hsp70 chaperone potentiate disaggregation of misfolded proteinsProceedings of the National Academy of SciencesDecember 7, 2021Hubert Wyszkowski, Anna Janta, Wiktoria Sztangierska, Igor Obuchowski, Tomasz Chamera, Agnieszka Kłosowska, Krzysztof Liberekkinase was purchased from Sigma-Aldrich (10127566001). Untagged luciferase was purchased from Promega (E1701). Luciferase Refolding Assay. Luciferase (1.875 mg/mL) was chemically denatured in the buffer A (25

(5) Compensatory ion transport buffers daily protein rhythms to regulate osmotic balance and cellular physiologyNature CommunicationsDecember 1, 2021Alessandra Stangherlin et al.a Cary Eclipse Fluorescence Spectrophotometer (Agilent) with 10 nM Quantilum (Promega, Madison, WI; E1701) in 30 mM MOPS, 10 mM β-mercaptoethanol, 1 mM ATP pH 7.4, 1 mM potassium luciferin, 10 mM MgSO4, and

(6) Design of SARS-CoV-2 hFc-Conjugated Receptor-Binding Domain mRNA Vaccine Delivered via Lipid NanoparticlesACS NanoJune 22, 2021Uri Elia et al.sera. MaxiSORP ELISA plates (Nunc) were precoated with recombinant luciferase (0.4 μg/mL, Promega, #E1701) or spike protein (2 μg/mL) overnight at 4 °C in carbonate buffer. Plated were washed three times with

(7) GmDNJ1, a type‐I heat shock protein 40 (HSP40), is responsible for both Growth and heat tolerance in soybeanPlant DirectJanuary 25, 2021Kwan‐Pok Li, Cheuk‐Hon Wong, Chun‐Chiu Cheng, Sau‐Shan Cheng, Man‐Wah Li, Sandra Mansveld, Alex Bergsma, Tengfang Huang, Michiel J. T. van Eijk, Hon‐Ming LamBio‐Rad). Luciferase refolding assay was performed as described (Zmijewski et al., 2004). Luciferase (E1701, Promega) was denatured at 42°C for 10 min before the assay. Then the denatured luciferase was preincubated

(8) Real-time monitoring of extracellular ATP in bacterial cultures using thermostable luciferasePLOS ONEJanuary 22, 2021Julian Ihssen, Nina Jovanovic, Teja Sirec, Urs Spitzorganisms. Materials and methods Chemicals and enzymes Wild-type Photinus pyralis luciferase (QuantiLum, E1701) was obtained from Promega. Engineered, thermostable luciferase (X-Shining™ luciferase, BX174908) and

(9) Development of near‐infrared firefly luciferin analogue reacted with wild‐type and mutant luciferasesChiralityJuly 1, 2020Nobuo Kitada, Ryohei Saito, Rika Obata, Satoshi Iwano, Kazuma Karube, Atsushi Miyawaki, Takashi Hirano, Shojiro A. Makiprovided by Kurogane Kasei Co., Ltd. and recombinant Ppy luciferase (QuantiLum® recombinant luciferase, E1701, Promega) was used. Wako Silica gel 70 F254 thin‐layer chromatography plates were used for analytical

(10) Synthesis of SMT022357 enantiomers and in vivo evaluation in a Duchenne muscular dystrophy mouse modelTetrahedronJanuary 10, 2020Babbs A, Berg A, Chatzopoulou M, Davies KE, Davies SG, Edwards B, Elsey DJ, Emer E, Figuccia ALA, Fletcher AM, et al.Firefly luciferase biochemical inhibition assay Recombinant firefly luciferase (QuantiLum Promega, E1701) was assayed at a final concentration of 0.6 nM in a buffer containing 25 mM HEPES, 5 mM MgCl2, 1 mM

(11) Characterisation of utrophin modulator SMT C1100 as a non-competitive inhibitor of firefly luciferaseBioorganic ChemistryJanuary 1, 2020Isabel V.L. Wilkinson, Jessica K. Reynolds, Sébastien R.G. Galan, Aini Vuorinen, Adam J. Sills, Elisabete Pires, Graham M. Wynne, Francis X. Wilson, Angela J. RussellBioscience Ltd), D-luciferin (Promega), ATP (Sigma), recombinant firefly luciferase (QuantiLum Promega, E1701). Ezutromid was provided by Summit Therapeutics plc. Analytical procedures and chemical synthesis of

(12) Adeno-Associated Virus Neutralizing Antibodies in Large Animals and Their Impact on Brain Intraparenchymal Gene TransferMolecular Therapy - Methods & Clinical DevelopmentDecember 14, 2018Dan Wang, Li Zhong, Mengxin Li, Jia Li, Karen Tran, Lingzhi Ren, Ran He, Jun Xie, Richard P. Moser, Cara Fraser, et al.#E1501) and compared against a standard curve generated using purified recombinant Fluc (Promega, cat. #E1701). The amount of Fluc in tissue lysate was calculated as femtograms (fg) of Fluc per μg of total protein. Statistical

(13) A high throughput screen for next-generation leads targeting malaria parasite transmissionNature CommunicationsDecember 1, 2018Michael J. Delves et al.1536-well opaque-bottom plates (789173-F, Greiner Bio-One). A 20 pM QuantiLum® Recombinant Luciferase (E1701, Promega) containing solution was made in screening media (DMEM, 11965–092, Invitrogen; 5% FBS, 35–011-CV,

(14) Bioengineered Viral Platform for Intramuscular Passive Vaccine Delivery to Human Skeletal MuscleMolecular Therapy - Methods & Clinical DevelopmentSeptember 21, 2018Nicole K. Paulk et al.normalized to molecules of FLuc using a dilution series of recombinant QuantiLum Firefly Luciferase (Promega E1701). Ex Vivo Transduction Analysis in Human and Rhesus Skeletal Muscle Fiber Explants Human or rhesus

(15) Alpha-defensin-dependent enhancement of enteric viral infectionPLOS PathogensJune 16, 2017Sarah S. Wilson, Beth A. Bromme, Mayumi K. Holly, Mayim E. Wiens, Anshu P. Gounder, Youngmee Sul, Jason G. SmithHAdV-5. eGFP (lanes 3 and 8, H5 eGFP) and recombinant firefly luciferase (lanes 4 and 9, rLuc, Promega E1701) were probed by immunoblot for GFP and luciferase. The IX-GFP fusion (41 kDa) is clearly distinguished

(16) In-depth Characterization of Firefly Luciferase as a Reporter of Circadian Gene Expression in Mammalian CellsJournal of Biological RhythmsJanuary 24, 2017Kevin A. Feeney, Marrit Putker, Marco Brancaccio, John S. O’Neillthroughout. Luciferase Assays in Solution QuantiLum is purified recombinant firefly luciferase (Promega, Madison, WI; E1701). QuantiLum was used at a concentration of 10 nM throughout, although we confirmed that substrate is

(17) A Simple Luminescent Adenylate-Cyclase Functional Assay for Evaluation of Bacillus anthracis Edema Factor ActivityToxins (Basel)August 18, 2016Ma’ayan Israeli, Shahar Rotem, Uri Elia, Erez Bar-Haim, Ofer Cohen, Theodor Chitlaruvarious experiments) at 30 ◦ C. Ten microliters of 1.437 µg/mL QuantiLum® Recombinant Luciferase (Promega E1701, Madison, WI, USA) were added to the mixture and luminescence was measured immediately with a luminometer

(18) A plant 35S CaMV promoter induces long-term expression of luciferase in Atlantic salmonScientific ReportsJuly 1, 2016Tore Seternes, Tom C. Tonheim, Anne I. Myhr, Roy A. DalmoAtlantic salmon. Material and Methods Luciferase Assay System (Cat # E1501) and QuantiLum® Recombinant luciferase (Cat # E1701) were obtained from Promega (Madison, WI, USA). Bio-Rad RC DC Protein Assay kit II (Cat # 500-0122)

(19) A reporter mouse for optical imaging of inflammation in mdx musclesSkeletal MuscleDecember 1, 2015Leonel Martinez, Natalia V Ermolova, Tomo-O Ishikawa, David B Stout, Harvey R Herschman, Melissa J Spencermembrane (100 V, 1 h, 4°C). As a positive control, 7.5 ng of QuantiLum recombinant luciferase (Promega, E1701) was used. Membranes were stained with Ponceau red and imaged and then blocked with 3% BSA and probed

(20) Two-Stage Prediction of the Effects of Imidazolium and Pyridinium Ionic Liquid Mixtures on LuciferaseMoleculesMay 23, 2014Hui-Lin Ge, Shu-Shen Liu, Bing-Xia Su, Xiang-Wei Zhuthe QuantiLum recombinant luciferase (cloned from North American firefly Photinus pyralis, Catalog #E1701, > 95% purity) and endotoxin-free D-luciferin (Catalog #E6551, ≥ 98.5% purity) from Promega (Madison,

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9/2/2015In Vivo Use of Recombinant Luciferase Alex BadamchiTransgene expression in vivo is often determined by luciferase expression in combination with luciferin and in vivo imaging software. Luciferase expression is usually presented as Relative ... Read Review