GoTaq Hot Start Polymerase from Promega

Product Specs
ItemGoTaq Hot Start Polymerase
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM5001
Concentration5u/μl
Quantity100u

Specifications/Features

Buffer:
5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Effect of bisphenol A on alterations of ICAM-1 and HLA-G genes expression and DNA methylation profiles in cumulus cells of infertile women with poor response to ovarian stimulationScientific ReportsDecember 1, 2021Somayeh Aftabsavad, Zahra Noormohammadi, Ashraf Moini, Morteza Karimipoorwas added into a 24 μL reaction mixture containing 0.5 U of hot start Gold Taq Polymerase (Cat NO: M5001, Promega, USA), 5 μL of the 10× PCR buffer, 2.0 μL of MgCl2 (50 mmol/L), 0.5 μL of dNTP (10 mmol/L;

(2) Detection of VAR2CSA-Captured Colorectal Cancer Cells from Blood Samples by Real-Time Reverse Transcription PCRCancers (Basel)November 23, 2021Sara R. Bang-Christensen, Viatcheslav Katerov, Amalie M. Jørgensen, Tobias Gustavsson, Swati Choudhary, Thor G. Theander, Ali Salanti, Hatim T. Allawi, Mette Ø. Agerbækthe M-MLV Reverse Transcriptase (Cat. no. M1701, Promega) and GoTaq® Hot Start Polymerase (Cat. no. M5001, Promega) combined with dNTPs and target specific primers as described above in a total volume of 25

(3) ColPortal, an integrative multiomic platform for analysing epigenetic interactions in colorectal cancerScientific DataDecember 1, 2019Angel Esteban-Gil et al.total volume of 20 μl containing 0.2 mM dNTPs, 2 mM MgCl2 0.025 U/μl GoTaq Hot Start Polymerase (ref: M5001, Promega, Madison, WI) and 0.5 μM of each primer. In the first PCR, 2 μl of DNA template were used with

(4) Molecular Characterisation of Equine Herpesvirus 1 Isolates from Cases of Abortion, Respiratory and Neurological Disease in Ireland between 1990 and 2017PathogensJanuary 15, 2019Marie Garvey, Rachel Lyons, Ralph D Hector, Cathal Walsh, Sean Arkins, Ann Cullinaneamplification of the target sequences consisted of 2.5 U GoTaq Hot Start Polymerase (5 U/µL) (catalogue no. M5001, Promega), 1X GoTaq Flexi Buffer, 2 mM MgCl2 solution, 0.2 mM dNTP Mixture (Applied Biosystems), 0.4

(5) An initial phase of JNK activation inhibits cell death early in the endoplasmic reticulum stress responseJournal of Cell ScienceApril 27, 2016Max Brown, Natalie Strudwick, Monika Suwara, Louise K. Sutcliffe, Adina D. Mihai, Ahmed A. Ali, Jamie N. Watson, Martin Schröderno. M7911), 1.5 mM MgCl2, 200 μM dNTPs, and 0.05 U/ml GoTaq hot start polymerase (Promega, cat. no. M5001). The reaction was incubated for 2 min at 94°C, and then cycled for 35 cycles consisting of subsequent

(6) Detection of Cryptosporidium parvum Oocysts on Fresh Produce Using DNA AptamersPLOS ONESeptember 3, 2015Asma Iqbal, Mahmoud Labib, Darija Muharemagic, Syed Sattar, Brent R. Dixon, Maxim V. BerezovskiμM reverse primer (5′-GGC TTC TGG ACT ACC TAT GC-3′) and 2.5 U of GoTaq Hot Start Polymerase (Cat. # M5001, Promega, WI, USA). The resulting amplification product was used as a template in the asymmetric PCR

(7) Identification of TRIML2, a novel p53 target, that enhances p53 SUMOylation and regulates the transactivation of proapoptotic genesMolecular cancer research : MCRFebruary 1, 2015Kung CP, Khaku S, Jennis M, Zhou Y, Murphy ME.analysis. Conventional PCR of ChIP products was performed with GoTaq® Hot Start Polymerase (Promega, M5001). Quantitative PCR (qPCR) was performed as described above. p53 responsive elements (REs) on TRIML2

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7/21/2016PCR Kit Benjamin GerovacThis kit is used for PCR of DNA fragments and is used for assessing if clones have inserts via colony PCR. The kit allows customization of magnesium concentration if a protocol calls for different ... Read Review