Phusion High-Fidelity DNA Polymerase from New England Biolabs

Product Specs
ItemPhusion High-Fidelity DNA Polymerase
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0530S
Quantity100 units
Concentration2,000 units/ml
FormatRecombinant
ApplicationsDNA Analysis
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeDNA Polymerase
Temperature-20 C

Specifications/Features

Buffer:

Phusion GC Buffer (5 X)
Phusion HF Reaction Buffer (5 X)

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) ATXN2 is a target of N-terminal proteolysisPLoS OneDecember 21, 2023Monika Chitre, Patrick Emerycases, we used standard PCR protocols with Taq, Phusion, or Q5 polymerases (New England Biolabs #M0273S,#M0530S, or #M0491S) to generate various vector and insert fragments. However, for CAG-repeat containing fragments,

(2) Reshaping the yeast galactose regulon via GPCR signaling cascadeCell Reports MethodsDecember 18, 2023Cong Fan, Jifeng YuanBiotech,Cat#B548101 Taq DNA Polymerase,NEB,Cat#M0267S Phusion High-Fidelity DNA Polymerase,NEB,Cat#M0530S Deoxynucleotide (dNTP) Solution Mix,NEB,Cat#N0447S BsaI-HFv2,NEB,Cat#R3733S T4 DNA Ligase,NEB,Cat#M0202S BamHI,NEB,Cat#R3136S SacI,NEB,Cat#R3156S Shrimp Alkaline Phosphatase (rSAP

(3) Orthologs of the Caenorhabditis elegans heterochronic genes have divergent functions in Caenorhabditis briggsaeGENETICSDecember 6, 2023Maria Ivanova, Eric G Mosstechniques. Q5 High-Fidelity DNA Polymerase (NEB #M0491S) and Phusion High-Fidelity DNA Polymerase (NEB #M0530S) were used for amplification. Cbr-lin-28 with promoter region and 3′UTR were amplified from the C. briggsae

(4) Noncoding mutations cause super-enhancer retargeting resulting in protein synthesis dysregulation during B cell lymphoma progressionNat GenetDecember 4, 2023Rebecca J. Leeman-Neill, Dong Song, Jonathan Bizarro, Ludivine Wacheul, Gerson Rothschild, Sameer Singh, Yang Yang, Aditya Y. Sarode, Kishore Gollapalli, Lijing Wu, et al.resuspension in ~120 µl H2O. 4C PCR was carried out using Phusion High-Fidelity DNA Polymerase (NEB, M0530S) and two sets of viewpoint primers containing the Illumina sequencing adaptor sequences (see sequences

(5) Structural basis of peptide secretion for Quorum sensing by ComANature CommunicationsNovember 7, 2023Lin Yu, Xin Xu, Wan-Zhen Chua, Hao Feng, Zheng Ser, Kai Shao, Jian Shi, Yumei Wang, Zongli Li, Radoslaw M. Sobota, et al.Scientific) at 37oC in 5% CO2. PCR products were synthesized using high fidelity Phusion DNA polymerase (NEB M0530S) and purified with the QIAquick PCR purification kit (Qiagen 28106) following the manufacturer’s protocol.

(6) MAST4 promotes primary ciliary resorption through phosphorylation of Tctex-1Life Science AllianceNovember 1, 2023Kensuke Sakaji, Sara Ebrahimiazar, Yasuhiro Harigae, Kenichi Ishibashi, Takeya Sato, Takeo Yoshikawa, Gen-ichi Atsumi, Ching-Hwa Sung, Masaki SaitoGreen Premix Ex Taq II (#RR820A) were purchased from Takara Bio. Phusion High-Fidelity DNA Polymerase (#M0530S) was purchased from New England Biolabs. The QuikChange Site-Directed Mutagenesis Kit (#200519) was

(7) The transcription activity of OTX2 on p16 expression is significantly blocked by methylation of CpG shore in non-promoter of lung cancer cell linesTranslational Cancer ResearchOctober 31, 2023Honghao Peng, Wenfan Fu, Chong Chang, Huan Gao, Qianmei He, Ziqi Liu, Mengxing Cui, Han Wang, Yongjiang Yu, Yonghui Wu, et al.fragment covering the sgRNA targeting sequence was amplified using Phusion high-fidelity DNA polymerase (M0530S; NEB, USA). The purified PCR product was used as a template to prepare a hybrid reaction system. After

(8) Human APOBEC3B promotes tumor development in vivo including signature mutations and metastasesCell Reports MedicineOctober 4, 2023Cameron Durfee, Nuri Alpay Temiz, Rena Levin-Klein, Prokopios P. Argyris, Lene Alsøe, Sergio Carracedo, Alicia Alonso de la Vega, Joshua Proehl, Anna M. Holzhauer, Zachary J. Seeman, et al.S1277 MluI-HF,New England Biolabs,Cat# R3198S Phusion high fidelity DNA polymerase,New England Biolabs,Cat# M0530S APOBEC3A-MycHis,Stenglein et al.62,N/A ,, Critical commercial assays,Critical commercial assays,Critical

(9) RNA folding studies inside peptide-rich droplets reveal roles of modified nucleosides at the origin of lifeScience AdvancesSeptember 22, 2023McCauley O. Meyer, Ryota Yamagami, Saehyun Choi, Christine D. Keating, Philip C. Bevilacquatriphosphates (dNTPs), 10 nM template ssDNA oligos, and 2 U Phusion HF DNA polymerase (New England Biolabs, M0530S). The PCR conditions were as follows: denature at 98°C for 5 s, anneal at 50°C for 10 s, elongate at

(10) Solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectorsNature GeneticsSeptember 1, 2023Xiao Lin, Yuxin Jia, Robert Heal, Maxim Prokchorchik, Maria Sindalovskaya, Andrea Olave-Achury, Moffat Makechemu, Sebastian Fairhead, Azka Noureen, Jung Heo, et al.genes, the ORF of each candidate genes was amplified by Phusion high-fidelity DNA polymerase (NEB, M0530S) or KAPA HiFi Uracil+ DNA polymerase (Roche, 07959052001) and then cloned into the pICLS86922 overexpression

(11) APOBEC3 degradation is the primary function of HIV-1 Vif determining virion infectivity in the myeloid cell line THP-1mBioAugust 31, 2023Ikeda T, Shimizu R, Nasser H, Carpenter MA, Cheng AZ, Brown WL, Sauter D, Harris RS.and 5´-NNN NAC GCG TCT AGC CTT CCC AGA CGT ACC C) using high-fidelity Phusion polymerase (NEB, Cat# M0530S). The PCR fragment was digested with BmtI and MluI, and ligated into the cut pLentiCRISPR1000, producing

(12) Fluorescent PCR-based Screening Methods for Precise Knock-in of Small DNA Fragments and Point Mutations in ZebrafishBio ProtocAugust 5, 2023Blake Carrington, Raman Soodsynthesis reagents Phusion High Fidelity DNA Polymerase and associated 5× buffer (NEB, catalog number: M0530S)Advantage UltraPure PCR dNTP mix (10 mM each) (Takara, catalog number: 639125)HiScribe T7 Quick High

(13) Nuclease-dead S. aureus Cas9 downregulates alpha-synuclein and reduces mtDNA damage and oxidative stress levels in patient-derived stem cell model of Parkinson's diseasebioRxivJuly 31, 2023Danuta Sastre, Faria Zafar, C. Alejandra Morato Torres, Desiree Piper, Deniz Kirik, Laurie H. Sanders, Stanley Qi, Birgitt SchüleSadCas9-2xKRAB-p2a segment was amplified by PCR from backbone using high-fidelity polymerase (Phusion, NEB, Cat. No. M0530S); and tdTomato was PCR amplified from a proprietary expression vector using high-fidelity polymerase.

(14) Aub, Vasa and Armi localization to phase separated nuage is dispensable for piRNA biogenesis and transposon silencing in DrosophilabioRxivJuly 29, 2023Samantha Ho, Nicholas P Rice, Tianxiong Yu, Zhiping Weng, William E TheurkaufPNK,NEB,Cat# M0201L Klenow 3ʹ to 5ʹ exo,NEB,Cat# M0212L UDG,NEB,Cat# M0280S Phusion Polymerase,NEB,Cat# M0530S "T4 RNA Ligase 2, truncated",NEB,Cat# M0242L 50% PEG8000,NEB,Cat# B1004S T4 DNA ligase,Enzymatics

(15) Proximity-dependent recruitment of Polycomb repressive complexes by the lncRNA AirnCell reportsJuly 25, 2023Braceros AK, Schertzer MD, Omer A, Trotman JB, Davis ES, Dowen JM, Phanstiel DH, Aiden EL, Calabrese JM.Acrylamide,Invitrogen,Cat#AM9520 Choice Taq,Denville,Cat#CB4050–2 Phusion High-Fidelity DNA Polymerase,New England Biolabs,Cat#M0530S Biotin-16-dUTP,Roche,Cat#11093070910 Terminal Deoxynucleotidyl Transferase,Thermo Scientific,Cat#EP0161 Klenow

(16) Involvement of skin TRPV3 in temperature detection regulated by TMEM79 in miceNature communicationsJuly 20, 2023Lei J, Yoshimoto RU, Matsui T, Amagai M, Kido MA, Tominaga M.adding restriction sites KpnI and NotI at two ends. The phusion high-fidelity DNA polymerase (NEB, M0530S) was used with a PCR thermal profile starting at 98 °C for 30 s, followed by 10 cycles at 98 °C for

(17) Large-scale Purification of Type III Toxin-antitoxin Ribonucleoprotein Complex and its Components from Escherichia coli for Biophysical StudiesBIO-PROTOCOLJuly 5, 2023Parthasarathy Manikandan, Kavyashree Nadig, Mahavir Singhkit, catalog number: 28104)Phusion High-Fidelity DNA polymerase (New England Biolabs, catalog number: M0530S), store at -20 °CPlasmid extraction kit (QIAprep® spin Miniprep kit, Qiagen, catalog number: 27106)Protease

(18) Biochemical and structural characterization of the first-discovered metazoan DNA cytosine-N4 methyltransferase from the Bdelloid rotifer Adineta vagaJournal of Biological ChemistryJuly 5, 2023Jujun Zhou, John R. Horton, Gundeep Kaur, Qin Chen, Xuwen Li, Fabian Mendoza, Tao Wu, Robert M. Blumenthal, Xing Zhang, Xiaodong Chengprimer (5′-CAG ACG TGT GCT CTT CCG ATC T-3′), 1 μl 2 U/μl Phusion High-Fidelity DNA Polymerase (NEB, M0530S), and 33.75 μl water). The solution was incubated at 94 °C for 5 min, 55 °C for 10 min, and 72 °C for

(19) A standardized quantitative analysis strategy for stable isotope probing metagenomicsmSystemsJune 28, 2023Dariia Vyshenska, Pranav Sampara, Kanwar Singh, Andy Tomatsu, W. Berkeley Kauffman, Erin E. Nuccio, Steven J. Blazewicz, Jennifer Pett-Ridge, Katherine B. Louie, Neha Varghese, et al.Briefly, DNA was amplified for 30 cycles by adding 0.5-µL Phusion high-fidelity (HF) DNA polymerase (NEB; M0530S), 10 µL of 5× Phusion HF Buffer, 1 µL of 10 mM dNTPs (final conc. labeled/unlabeled mixture), 2.5 µL

(20) Metatranscriptomics analysis reveals a novel transcriptional and translational landscape during Middle East respiratory syndrome coronavirus infectioniScienceJune 16, 2023Ethan J. Fritch, Wes Sanders, Amy C. Sims, Laura E. Herring, Natalie K. Barker, Athena A. Schepmoes, Karl K. Weitz, Jordan R. Texier, Dirk P. Dittmer, Lee M. Graves, et al.amplicons using a genome-spanning PCR primer set (Table S6) using the Phusion PCR enzyme kit (NEB Cat. M0530S). Amplicons were pooled using a Zymo DNA Clean and Concentrator kit (Genesee Scientific Cat. 11-303C).

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(1)

6/27/2016PCR Amplification of DNA Templates for Cloning Triveni MahendrappaI have used phusion high fidelity polymerase for amplification of DNA templates/genes needed for cloning. I got good quality amplification products which are error free. Quantity obtained was ... Read Review
(1)

3/30/2016PCR Using Phusion High Fidelity DNA PolymeraseDr. Thippeswamy SannasiddappaI have used Phusion high fidelity DNA polymerase for amplification of DNA inserts for cloning experiments. As a rule of thumb, I wanted to go with thermo-stable polymerase having 50 fold less error ... Read Review