HaeIII from New England Biolabs

Product Specs
ItemHaeIII
CompanyNew England Biolabs
Price $70.00 Inquire
Catalog NumberR0108S
Concentration10-50 u/µL
Quantity3000 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

Sequence

GG/CC

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Public Spaces as Hotspots of Zoonotic Gastrointestinal Parasite Transmission: Evidence from Small Animal and Soil Surveillance in MalaysiaJournal of Epidemiology and Global HealthJanuary 16, 2026Suey Yee Low, Sze Kee Gun, Sadiq Mohammed Babatunde, Sharifah Salmah Syed Hussain, Wan Nur Ismah Wan Ahmad Kamil, Abdul Rahman Omar, Azalea Hani Othman, Tengku Rinalfi Putra Tengku Azizan, Maizatul Akmal Moktar, Noraain binti Azman, et al.min. Amplified PCR products of RTGHF1-RTGHR1 were subjected to digestion of HinF1 (catalog number: R0108S, New England Biolabs) and RSaI (catalog number: R0167S, New England Biolabs) to differentiate its species

(2) Systematic enhancer mapping and functional analysis in zebrafish with optimized CRISPR interferenceNucleic Acids ResDecember 11, 2025Jiulin Chan, Zhichao Wu, Mingli Liu, Tianming Wang, Hanyu Liu, Ruimeng Cao, Xiaolong Li, Xinwen Li, Siyao Zhan, Jiangbo Cheng, et al.Triton X-100 containing rCutSmart™ Buffer (NEB, Cat#B6004V) and 400 units of HaeIII and AluI (NEB, Cat#R0108S and Cat#R0137) and incubating overnight at 37°C. A-tailing, proximity ligation, and linker removal Digestion

(3) Genome editing with the HDR-enhancing DNA-PKcs inhibitor AZD7648 causes large-scale genomic alterationsNature BiotechnologyNovember 1, 2025Grégoire Cullot, Eric J. Aird, Moritz F. Schlapansky, Charles D. Yeh, Lilly van de Venn, Iryna Vykhlyantseva, Susanne Kreutzer, Dominic Mailänder, Bohdan Lewków, Julia Klermund, et al.and reverse primer, 250 nM probe; Bio-Rad) and 10 U µg−1 DNA of the HaeIII restriction enzyme (NEB, R0108S) cutting outside of the amplicons. Generated droplets were then subjected to thermal cycling (10 min

(4) Selfish mutations promote age-associated erosion of mtDNA integrity in mammalsNat CommunJuly 1, 2025Ekaterina Korotkevich, Daniel N. Conrad, Zev J. Gartner, Patrick H. O'Farrellreverse primer, 250 nM of WT probe, 250 nM of mutant probe, 0.5 μL of restriction enzyme (HaeIII; NEB, R0108L) and template DNA. Template DNA concentration was adjusted to be below 3500 mtDNA copies per microliter

(5) Ultra-rapid droplet digital PCR enables intraoperative tumor quantificationMedJune 13, 2025Zachary R. Murphy, Emilia C. Bianchini, Andrew Smith, Lisa I. Körner, Teresa Russell, David Reinecke, Nader Maarouf, Yuxiu Wang, John G. Golfinos, Alexandra M. Miller, et al.Taq DNA polymerase, 100 U/μL, high concentration",NEB,Cat# M0495B-HC3 (custom order) HaeIII,NEB,Cat# R0108S ddPCR supermix for probes (no dUTP),Bio-Rad,Cat# 1863025 ddPCR buffer control kit for probes,Bio-Rad,Cat#

(6) A specific pluripotency-associated eRNA controls Nanog locus by shaping the epigenetic landscape and stabilizing enhancer-promoter interactionNucleic Acids ResApril 12, 2025Mariella Cuomo, Davide Costabile, Rosa Della Monica, Michela Buonaiuto, Federica Trio, Giulia De Riso, Roberta Visconti, Lorenzo Chiariottidescribed in [26]. Lysed nuclei were digested with 400 U HaeIII restriction enzyme (New England Biolabs, R0108S) and ligated with 100 U of T4 DNA ligase (New England Biolabs, M0202S) for 4 h at 16°C followed by 30

(7) Chromosomal domain formation by archaeal SMC, a roadblock protein, and DNA structureNat CommunFebruary 19, 2025Kodai Yamaura, Naomichi Takemata, Masashi Kariya, Ayami Osaka, Sonoko Ishino, Masataka Yamauchi, Tomonori Tamura, Itaru Hamachi, Shoji Takada, Yoshizumi Ishino, et al.μL of MilliQ water. This was further mixed with 15 μL each of 10 U/μL HaeIII (New England Biolabs, R0108L) and 10 U/μL AluI (New England Biolabs, R0137L) and incubated for 3 h at 37 °C with agitation at 600

(8) The Impact of Human Salivary Amylase Gene Copy Number and Starch on Oral BiofilmsMicroorganismsFebruary 19, 2025Dorothy K. Superdock, Lynn M. Johnson, Jennifer Ren, Alizeh Khan, Megan Eno, Shuai Man, Angela C. Pooledroplet PCR (ddPCR), genomic DNA was digested with HaeIII (New England Biolabs, Ipswich, MA, USA, cat # R0108S). The DNA was diluted to 15 ng/µL before ddPCR was performed in duplicate reactions using Life Technologies

(9) Gut microbial features and dietary fiber intake predict gut microbiota response to resistant starch supplementationGut MicrobesDecember 31, 2024Sri Lakshmi Sravani Devarakonda, Dorothy K. Superdock, Jennifer Ren, Lynn M. Johnson, Aura (Alex) P. Loinard-González, Angela C. Pooledigital PCR, genomic DNA was digested with the restriction enzyme HaeIII (New England Biolabs, cat # R0108S) and diluted to a final concentration of approximately 15 ng/µl. Digital PCR was performed using Life

(10) Type I IFN drives unconventional IL-1β secretion in lupus monocytesImmunityNovember 12, 2024Caielli S, Balasubramanian P, Rodriguez-Alcazar J, Balaji U, Robinson L, Wan Z, Baisch J, Smitherman C, Walters L, Sparagana P, et al.A1,Invivogen,Cat#tlrl-baf1 Disulfiram,Santa Cruz Biotechnology,Cat#sc-205654 Haelll,New England Biolabs,Cat#R0108S BS(PEG)5 (PEGylated bis(sulfosuccinimidyl)suberate),Thermo-Fisher,Cat#21581 holo-Transferrin,Santa Cruz Biotechnology

(11) Selection promotes age-dependent degeneration of the mitochondrial genomebioRxivOctober 3, 2024Ekaterina Korotkevich, Daniel N. Conrad, Zev J. Gartner, Patrick H. O’Farrellreverse primer, 250 nM of WT probe, 250 nM of mutant probe, 0.5 μL of restriction enzyme (HaeIII; NEB, R0108L) and template DNA. Template DNA concentration was adjusted to be below 3,500 mtDNA copies per microliter

(12) Rbpms2 promotes female fate upstream of the nutrient sensing Gator2 complex component MiosNature communicationsJune 19, 2024Wilson ML, Romano SN, Khatri N, Aharon D, Liu Y, Kaufman OH, Draper BW, Marlow FL.temperature of 60 °C and the wildtype allele was digested with HaeIII for 1 h (New England Biolabs, R0108S)2. The rbpms2bsa9329 surrounding genomic region was amplified for 35 cycles with an annealing temperature

(13) A conserved Pol II elongator SPT6L mediates Pol V transcription to regulate RNA-directed DNA methylation in ArabidopsisNature CommunicationsMay 25, 2024Yujuan Liu, Jie Shu, Zhi Zhang, Ning Ding, Jinyuan Liu, Jun Liu, Yuhai Cui, Changhu Wang, Chen Chengenomic DNA was digested overnight with methylation-sensitive restriction endonucleases (Hae III, NEB, R0108S). The digested DNA was used to amplify the indicated regions by PCR using primers flanking the endonuclease

(14) Validation of Single‐Nucleotide Mosaic Variants Through Droplet Digital PCRCurrent ProtocolsMay 22, 2024Christopher M. Grochowski, Richard A. Gibbs, Harsha Doddapanenifragmentation of the template >66 ng per reaction: HaeIII (New England Biolabs, NEB, Catalogue Number: R0108S), MseI (NEB, Catalogue Number: R0525S), HindIII (NEB, Catalogue Number: R3104S), are recommended by

(15) Digital droplet PCR analysis of organoids generated from mouse mammary tumors demonstrates proof-of-concept capture of tumor heterogeneityFrontiers in Cell and Developmental BiologyMay 15, 2024Katherine E. Lake, Megan M. Colonnetta, Clayton A. Smith, Kaitlyn Saunders, Kenneth Martinez-Algarin, Sakshi Mohta, Jacob Pena, Heather L. McArthur, Sangeetha M. Reddy, Evanthia T. Roussos Torres, et al.supermix (no dUTP) (Bio-Rad; cat: 1863024), HaeIII restriction enzyme and rCutsmart buffer (NEB; cat: R0108S), and nuclease-free water were mixed with primer/probe for target and reference gene according to manufacturer

(16) BL-Hi-C reveals the 3D genome structure of Brassica crops with high sensitivityHorticulture ResearchJanuary 16, 2024Lupeng Zhang, Ranze Zhao, Jianli Liang, Xu Cai, Lei Zhang, Huiling Guo, Zhicheng Zhang, Jian Wu, Xiaowu Wanggently shaken for 15 minutes at 37°C. Then, 25 μl of 10 × CutSmart Buffer and 10 μl of Hae III (NEB, #R0108L) were added to each tube, and the tubes were incubated at 37°C for 12 h with rotation at 900 r.p.m.

(17) A Germinal Center Checkpoint of AIRE in B Cells Limits Antibody DiversificationbioRxivJanuary 15, 2024Jordan Z. Zhou, Bihui Huang, Bo Pei, Guang Wen Sun, Michael D. Pawlitz, Wei Zhang, Xinyang Li, Kati C. Hokynar, Fayi Yao, Madusha L. W. Perera, et al.,Cayman Chemical,Cat#10009350 RNase A,Sigma,Cat# R6513 Proteinase K,Qiagen,Cat# 9133 HaeIII,New England Biolabs,Cat# R0108S O-Allylhydroxylamine hydrochloride (AA7),Sigma,Cat# 05983 Methoxyamine hydrochloride,Sigma,Cat# 226904 Alkoxyamine-6

(18) Nuclear growth and import can be uncoupledbioRxivApril 25, 2023Pan Chen, Sampada Mishra, Daniel L. Levy(NEB, M0247S), 0.2 U/μL EcoRI (NEB, R0101S), 0.2 U/μL XhoI (NEB, R0101S), or 0.5 U/μL HaeIII (NEB, R0108S) was added to X. laevis egg extract after nuclear assembly. WGA (Sigma, L9640) and 150-kDa FITC-dextran

(19) The conserved histone chaperone Spt6 is strongly required for DNA replication and genome stabilityCell ReportsMarch 28, 2023Catherine L.W. Miller, Fred Winston(IAA),Sigma,12886 RnaseH,NEB,M0297L AccI,NEB,R0161S EcoRV,NEB,R3195S NcoI,NEB,R3193S HaeIII,NEB,R0108S BsrG1,NEB,R3575S SigmaFAST protease inhibitor cocktail tablets,Sigma,S8820-20TAB RNaseA/T1 Mix,Thermo,EN0551 Benzonase,Sigma,E1014-5KU Dynabeads

(20) Characterization of a Novel Thermostable DNA Lyase Used To Prepare DNA for Next-Generation SequencingChemical research in toxicologyFebruary 20, 2023Baljinnyam T, Conrad JW, Sowers ML, Chang-Gu B, Herring JL, Hackfeld LC, Zhang K, Sowers LC.Mismatch-specific endonuclease I (MSE, #M0678S) and restriction enzymes HpaII (#R0171S) and HaeIII (#R0108S) were also purchased from NEB. Human 8-oxoguanine DNA glycosylase (hOGG1, #NBP1-45318-0.1mg) and DNA

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HaeIII

HaeIII from New England Biolabs

Sequence

New England Biolabs

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HaeIII from New England Biolabs

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