NovoCyte Quanteon Flow Cytometer from Agilent Technologies

Product Specs
ItemNovoCyte Quanteon Flow Cytometer
CompanyAgilent Technologies
Price Get Pricing Get Quote for this Product
Catalog NumberNovoCyte Quanteon
QuantityEA
Abs / Exc405 nm, 488 nm, 561 nm, 637 nm
Detection25 independent photomultipliers
ApplicationsCell Cycle Analysis / Immunophenotyping / Lymphocyte Count
Sample Type12 × 75 mm tubes, 1.5 and 2 mL tubes, 24-well, 48-well, 96-well and 384-well microtiter plates
Dimensions33.5 × 18.1 × 18.8 in. (85 × 46 × 48 cm) with Agilent NovoSampler Q
Voltage100 / 115 / 230 VAC, 50 to 60 Hz
Flow Rate5 to 120 µl/min

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Agilent Technologies

5301 Stevens Creek Blvd

Santa Clara, CA 95051

United States

Phone: +1 877-424-4536

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Website: Agilent Technologies

(1) Targeting CD70 in combination with chemotherapy to enhance the anti-tumor immune effects in non-small cell lung cancerOncoImmunologyDecember 31, 2023Tal Flieswasser, Astrid Van den Eynde, Laurie Freire Boullosa, Jöran Melis, Christophe Hermans, Céline Merlin, Ho Wa Lau, Jonas Van Audenaerde, Filip Lardon, Evelien Smits, et al.and Live/Dead Fixable Aqua (1:50, Invitrogen) for 30 minutes at 4°C. Acquisition was performed on the Novocyte Quanteon (Agilent technologies). Cytokine secretion Supernatant was collected from the co-cultures of NSCLC

(2) Gastric protective effect of Alpinia officinarum flavonoids: mediating TLR4/NF-κB and TRPV1 signalling pathways and gastric mucosal healingPharmaceutical BiologyDecember 31, 2023Kaiwen Lin, Tang Deng, Huijuan Qu, Hongya Ou, Qifeng Huang, Bingmiao Gao, Xiaoliang Li, Na WeiShanghai, China) was added as instructed, and apoptosis was detected using Agilent flow cytometry (NovoCyte Quanteon, San Diego, CA). RNA extraction and RNA-seq The normal group, acetic acid model group and F.AOH middle

(3) LTX-315 and adoptive cell therapy using tumor-infiltrating lymphocytes generate tumor specific T cells in patients with metastatic soft tissue sarcomaOncoimmunologyDecember 21, 2023Morten Nielsen, Tine Monberg, Vibeke Sundvold, Benedetta Albieri, Dorrit Hovgaard, Michael Mørk Petersen, Anders Krarup-Hansen, Özcan Met, Ketil Camilio, Trevor Clancy, et al.was performed to assess the phenotype and tumor reactivity of in vitro expanded TILs and PBMCs on a NovoCyte Quanteon Flow Cytometer (Agilent Technologies). Cells were washed in PBS (Lonza) and stained with fluorochrome-conjugated antibodies

(4) Tumor Immune Cell Targeting Chimeras (TICTACs) For Targeted Depletion of Macrophage-Associated Checkpoint ReceptorsbioRxivDecember 6, 2023Mariko Morimoto, Nicholas A. Till, Carolyn R. Bertozzifor 10 min at 4 °C. Flow cytometry was performed on either a MACSQuant Analyzer 10 (Miltenyi Biotec), Novocyte Quanteon (Agilent), or Novocyte Penteon (Agilent), and FlowJo software was used to gate on single cells, live

(5) Recruitment of naive CD4+ T cells by the recombinant zoster vaccine correlates with persistent immunityJournal of Clinical InvestigationDecember 1, 2023Kerry J. Laing, Emily S. Ford, Michael J. Johnson, Myron J. Levin, David M. Koelle, Adriana WeinbergBD Biosciences), and anti-CD8–PE-CF594 (clone RPA-T8, BD Biosciences). Cells were acquired using the NovoCyte Quanteon flow cytometer (Agilent Technologies), and the Tet+ cells were identified in the live, single-cell, CD3+CD8–CD4+CTVlo

(6) Boosting cytotoxicity of adoptive allogeneic NK cell therapy with an oncolytic adenovirus encoding a human vIL-2 cytokine for the treatment of human ovarian cancerCancer Gene TherapyNovember 10, 2023D. C. A. Quixabeira, S. Pakola, E. Jirovec, R. Havunen, S. Basnet, J. M. Santos, T. V. Kudling, J. H. A. Clubb, L. Haybout, V. Arias, et al.Transcription Factor Buffer Set (424401, BD, NJ, USA) protocol. Cell fluorescence was acquired with NovoCyte Quanteon Flow Cytometer Systems (Agilent, CA, USA), upon acquisition of 90k to 100k events per well. Cell gating and data processing

(7) Fanconi anemia pathway regulation by FANCI in prostate cancerFrontiers in OncologyOctober 30, 2023Heidi Kaljunen, Sinja Taavitsainen, Roosa Kaarijärvi, Eerika Takala, Ville Paakinaho, Matti Nykter, G. Steven Bova, Kirsi KetolaThe cells were analyzed in 1× PBS with 2% FBS included. The fluorescence of cells was measured using NovoCyte Quanteon Flow Cytometer (Agilent, Santa Clara, CA, USA) with NovoExpress® software (Agilent). The analysis of the results was

(8) Spindle architecture constrains karyotype in budding yeastbioRxivOctober 29, 2023Jana Helsen, Md Hashim Reza, Gavin Sherlock, Gautam Deystrain were used as controls, and fluorescence of 30,000 cells was analyzed by flow cytometry on an Acea Novocyte Quanteon (Agilent). Ultrastructure expansion microscopy Ultrastructure expansion microscopy (U-ExM) was performed

(9) Angiopoietin-2 blockade suppresses growth of liver metastases from pancreatic neuroendocrine tumors by promoting T cell recruitmentJournal of Clinical InvestigationOctober 16, 2023Eunhyeong Lee, Sophie O’Keefe, Alessandra Leong, Ha-Ram Park, Janani Varadarajan, Subrata Chowdhury, Shannon Hiner, Sungsoo Kim, Anahita Shiva, Richard A. Friedman, et al.the cell staining. Cells were subsequently washed, resuspended in FACS buffer, and analyzed using the Novocyte Quanteon flow cytometry system (Agilent). Flow cytometry analyses of cell populations were performed using FlowJo

(10) Prior SARS-CoV-2 Infection Enhances Initial mRNA Vaccine Response with a Lower Impact on Long-Term ImmunityImmunoHorizonsOctober 11, 2023Vanessa Silva-Moraes, Aisha Souquette, Giuseppe A. Sautto, Ida Paciello, Giada Antonelli, Emanuele Andreano, Rino Rappuoli, Andréa Teixeira-Carvalho, Ted M. Ross(OX40+CD40L+) cells. Full gating strategy can be found in Supplemental Fig. 1A. All samples were acquired on a NovoCyte Quanteon flow cytometer system with four lasers (Agilent Technologies, Santa Clara, CA). A list of Abs used in this panel can

(11) Transcriptional network governing extraembryonic endoderm cell fate choiceDevelopmental BiologyOctober 1, 2023Paula Duyen Pham, Hanbin Lu, Han Han, Jeff Jiajing Zhou, Aarushi Madan, Wenqi Wang, Cornelis Murre, Ken W.Y. Choanalyzing the expression of NeonG fluorescence protein in FKBP12F36V knock-in lines using the ACEA Novocyte Quanteon (Agilent). For cell surface staining, cells were dissociated, washed in 1X PBS, stained with anti-CD140A (PDGFRA

(12) CCL21-DC in situ vaccination in murine NSCLC overcomes resistance to immunotherapy and generates systemic tumor-specific immunityJournal for ImmunoTherapy of CancerSeptember 19, 2023Ramin Salehi-Rad, Raymond J Lim, Yushen Du, Linh M Tran, Rui Li, Stephanie L Ong, Zi Ling Huang, Camelia Dumitras, Tianhao Zhang, Stacy J Park, et al.permeabilization buffer set (eBioscience). Data acquisition was performed on Attune NxT (ThermoFisher) or NovoCyte Quanteon (Agilent) cytometer, and data analyzed by FlowJo software (TreeStar). Antibodies are detailed in online

(13) Integrative dissection of 5-hydroxytryptamine receptors-related signature in the prognosis and immune microenvironment of breast cancerFrontiers in OncologySeptember 19, 2023Dandan Zhan, Xuan Wang, Yifeng Zheng, Shengqi Wang, Bowen Yang, Bo Pan, Neng Wang, Zhiyu Wangassay and CFSE staining assay The FACSAria III flow cytometer (BD Biosciences, California, US) or the NovoCyte Quanteon Flow cytometer (Agilent Technologies, California, USA) was used to analyze and sort the population of CD8+ T cells.

(14) A new osteogenic protein isolated from Dioscorea opposita Thunb accelerates bone defect healing through the mTOR signaling axisBioactive materialsSeptember 1, 2023Kubi JA, Brah AS, Cheung KMC, Lee YL, Lee KF, Sze SCW, Qiao W, Yeung KW.fluorescent labeled CD206, and MGL-1 antibodies (Cell Signaling) for flow cytometry analysis using an Agilent NovoCyte Quanteon analyzer. 2.16 Effects of HKUOT-S2 treatment on osteoblast biomineralization The hMSCs seeded were

(15) Precision targeting of autoantigen-specific B cells in muscle-specific tyrosine kinase myasthenia gravis with chimeric autoantibody receptor T cellsNature biotechnologySeptember 1, 2023Oh S, Mao X, Manfredo-Vieira S, Lee J, Patel D, Choi EJ, Alvarado A, Cottman-Thomas E, Maseda D, Tsao PY, et al.0.5 μg ml−1 propidium iodide in the presence of precision count beads before flow cytometry (Agilent NovoCyte Quanteon, FlowJo software v.10). Absolute count of live target cells was normalized to absolute bead count. Measurement

(16) Development of a Robust Consensus Modeling Approach for Identifying Cellular and Media Metabolites Predictive of Mesenchymal Stromal Cell PotencyStem CellsAugust 16, 2023Alexandria Van Grouw, Maxwell B Colonna, Ty S Maughon, Xunan Shen, Andrew M Larey, Samuel G Moore, Carolyn Yeago, Facundo M Fernández, Arthur S Edison, Steven L Stice, et al.overnight at 4 °C in the dark until flow analysis. All flow cytometry experiments were performed using a Novocyte Quanteon flow cytometer (Agilent) with 20 000 events collected per sample. All flow cytometry data were analyzed using FlowJo

(17) Influenza vaccine format mediates distinct cellular and antibody responses in human immune organoidsImmunityAugust 8, 2023Kastenschmidt JM, Sureshchandra S, Jain A, Hernandez-Davies JE, de Assis R, Wagoner ZW, Sorn AM, Mitul MT, Benchorin AI, Levendosky E, et al.buffer for 30 minutes on ice while protected from light. Data were collected using a Quanteon ACEA NovoCyte Quanteon (Agilent) flow cytometer. Cell sorting was performed using a BD FACSAria Fusion instrument. Flow cytometry analysis

(18) Pulmonary interleukin 1 beta/serum amyloid A3 axis promotes lung metastasis of hepatocellular carcinoma by facilitating the pre-metastatic niche formationJournal of Experimental & Clinical Cancer ResearchJuly 13, 2023Chong Zhang, Qing Li, Qi Xu, Wei Dong, Chunmei Li, Bin Deng, Jiao Gong, Li-Zhen Zhang, Junfei Jinwith PE anti-mouse IL-1β after fixation and permeabilization. FACS analysis was performed on Agilent NovoCyte Quanteon Flow Cytometer. Lentivirus production and lentiviral infection in the lungs of mice HEK293T cells were used to produce

(19) Deep Characterization and Comparison of Different Retrovirus-like Particles Preloaded with CRISPR/Cas9 RNPsInternational Journal of Molecular SciencesJuly 13, 2023Max Wichmann, Cecile L. Maire, Niklas Nuppenau, Moataz Habiballa, Almut Uhde, Katharina Kolbe, Tanja Schröder, Katrin Lamszus, Boris Fehse, Dawid Główcentrifugation at 310× g for 5 min and resuspension of the pellet in PBS. FC analysis was carried out using a NovoCyte Quanteon Flow Cytometer (Agilent Technologies, Santa Clara, CA, USA). Data were analyzed using BD FACSDiva and FlowJo (both

(20) Allosteric Inhibitors of Macrophage Migration Inhibitory Factor (MIF) Interfere with Apoptosis-Inducing Factor (AIF) Co-Localization to Prevent ParthanatosJournal of medicinal chemistryJuly 13, 2023Chen D, Osipyan A, Adriana J, Kader M, Gureev M, Knol CWJ, Sigmund MC, Xiao Z, van der Wouden PE, Cool RH, et al.cells were stained with 7 μM Hoechst 33342 and 2 μM propidium iodide (PI), and then measured using NovoCyte Quanteon (Agilent, Santa Clara). The cells stained only with Hoechst 33342 were counted as live cells, while

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