NEB 10-beta Electrocompetent E.coli from New England Biolabs

Product Specs
ItemNEB 10-beta Electrocompetent E.coli
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberC3020K
Quantity6 x 0.1 ml
GenotypearaD139 Δ(ara-leu)7697 fhuA lacX74 galK (Φ80 Δ(lacZ)M15) mcrA galU recA1 endA1 nupG rpsL Δ(mrr-hsdRMS-mcrBC)
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
Cell Type (standardized)Competent Cells
Validated Species ReactivityEscherichia
SpeciesE.coli
Temperature-80 C
Cell TypeCompetent Cells

Specifications/Features

Transformation Efficiency:
1-3 x 10¹⁰ cfu/µg pUC19

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Mapping the neutralizing specificity of human anti-HIV serum by deep mutational scanningCell Host & MicrobeJuly 12, 2023Caelan E. Radford, Philipp Schommers, Lutz Gieselmann, Katharine H.D. Crawford, Bernadeta Dadonaite, Timothy C. Yu, Adam S. Dingens, Julie Overbaugh, Florian Klein, Jesse D. Bloomμl of the purified HiFi product to transform 20 μl of 10-beta electrocompetent E. coli cells (NEB, C3020K). We performed 5 electroporation reactions for a final count of >5 million CFUs per library. We aimed

(2) Investigating pioneer factor activity and its coordination with chromatin remodelers using integrated synthetic oligo assaySTAR ProtocolsJune 7, 2023Hengye Chen, Chao Yan, Archana Dhasarathy, Michael Kladde, Lu Baistrains,Bacterial and virus strains ,, NEB® 10-beta Electrocompetent E. coli,New England Biolabs,C3020K ,, "Chemicals, peptides, and recombinant proteins","Chemicals, peptides, and recombinant proteins","Chemicals,

(3) Optimized metrics for orthogonal combinatorial CRISPR screensScientific ReportsMay 6, 2023Ronay Cetin, Martin Wegner, Leah Luwisch, Sarada Saud, Tatjana Achmedov, Sebastian Süsser, Antonella Vera-Guapi, Konstantin Müller, Yves Matthess, Eva Quandt, et al.synthesis product was transformed into 400 µl of electrocompetent E. coli (10-beta, New England Biolabs, C3020K) cells with Bio-Rad Gene Pulser (voltage 2.5 kV, capacitance 25 µF and resistance 200 Ω). The cells

(4) Transcription factor binding site orientation and order are major drivers of gene regulatory activityNature CommunicationsApril 22, 2023Ilias Georgakopoulos-Soares, Chengyu Deng, Vikram Agarwal, Candace S. Y. Chan, Jingjing Zhao, Fumitaka Inoue, Nadav AhituvI-SceI (R0694S, NEB) digestion. Recombination products were transformed to electrocompetent cells (C3020K, NEB) following manufacture’s protocol and incubated overnight at 37°C on 15 cm LB agar plates with

(5) Mapping the neutralizing specificity of human anti-HIV serum by deep mutational scanningbioRxivMarch 30, 2023Caelan E. Radford, Philipp Schommers, Lutz Gieselmann, Katharine H. D. Crawford, Bernadeta Dadonaite, Timothy C. Yu, Adam S. Dingens, Julie Overbaugh, Florian Klein, Jesse D. Bloomµl of the purified HiFi product to transform 20 µl of 10-beta electrocompetent E. coli cells (NEB, C3020K). We performed 5 electroporation reactions for a final count of >5 million CFUs per library. We aimed

(6) Regulatory and coding sequences of TRNP1 co-evolve with brain size and cortical folding in mammalseLifeMarch 22, 2023Zane Kliesmete, Lucas Esteban Wange, Beate Vieth, Miriam Esgleas, Jessica Radmer, Matthias Hülsmann, Johanna Geuder, Daniel Richter, Mari Ohnuki, Magdelena Götz, et al.1a "Strain, strain background (E. coli)",NEB 10-beta,"New England Biolabs; Rowley, MA, United States",Cat# C3020K,Electrocompetent E. coli "Strain, strain background (E. coli)",NEB 5-alpha High Efficiency,"New England Biolabs

(7) A pseudovirus system enables deep mutational scanning of the full SARS-CoV-2 spikeCellMarch 16, 2023Bernadeta Dadonaite, Katharine H.D. Crawford, Caelan E. Radford, Ariana G. Farrell, Timothy C. Yu, William W. Hannon, Panpan Zhou, Raiees Andrabi, Dennis R. Burton, Lihong Liu, et al.μl of the purified HiFi product to transform 20 μl of 10-beta electrocompetent E. coli cells (NEB, C3020K). We performed 10 electroporation reactions to get a final count of > 2 million CFUs per library and

(8) How Epstein-Barr Virus Induces the Reorganization of Cellular ChromatinmBioFebruary 28, 2023Rosemarie Q, Kirschstein E, Sugden B.Resulting Gibson mixes were transformed into NEB 10-beta electrocompetent E. coli (NEB; catalog no. C3020K) and selected by plating onto agar plates containing 100 μg/mL carbenicillin. The integrity of the resulting

(9) Selection of Extended CRISPR RNAs with Enhanced Targeting and SpecificitybioRxivJanuary 15, 2023Ashley Herring-Nicholas, Hillary Dimig, Miranda Roesing, Eric A. Josephsand E. coli strains All cloning was performed using New England Biolabs (NEB) 10-beta cells (NEB #C3020K) or TOP10 (Invitrogen #C404010) cells, and all SECRETS assays performed in Stbl2 cells (Invitrogen #10268019),

(10) Whole-genome functional characterization of RE1 silencers using a modified massively parallel reporter assayCell GenomicsJanuary 11, 2023Kousuke Mouri, Hannah B. Dewey, Rodrigo Castro, Daniel Berenzy, Susan Kales, Ryan Tewheymixed with a 1500:1 ratio of molarity. The mixture electroporated into 100 μL of 10-beta E.coli (NEB, C3020K, 2kV, 200 ohm, 25 μF) in order to achieve a transformation efficiency equal to 300-times the number

(11) Deep mutational scans for ACE2 binding, RBD expression, and antibody escape in the SARS-CoV-2 Omicron BA.1 and BA.2 receptor-binding domainsPLoS PathogNovember 18, 2022Tyler N. Starr, Allison J. Greaney, Cameron M. Stewart, Alexandra C. Walls, William W. Hannon, David Veesler, Jesse D. Bloomhttps://github.com/jbloomlab/SARS-CoV-2-RBD_DMS_Omicron/blob/main/data/3294lib_pETcon-SARS2-RBD_Omicron-BA1_lib-assembled.gb. Assembled library plasmids were electroporated into E. coli (NEB 10-beta, New England Biolabs C3020K), and plated at limiting dilutions on LB+ampicillin plates. For each library, duplicate plates corresponding

(12) Compensatory epistasis maintains ACE2 affinity in SARS-CoV-2 Omicron BA.1Nat CommunNovember 16, 2022Alief Moulana, Thomas Dupic, Angela M. Phillips, Jeffrey Chang, Serafina Nieves, Anne A. Roffler, Allison J. Greaney, Tyler N. Starr, Jesse D. Bloom, Michael M. DesaiAssembly products were transformed into NEB 10-beta electrocompetent E. coli cells (NEB, Ipswich, MA, #C3020K), following the manufacturer protocol. After overnight incubation at 37 °C, the cells were harvested,

(13) A pseudovirus system enables deep mutational scanning of the full SARS-CoV-2 spikebioRxivOctober 15, 2022Bernadeta Dadonaite, Katharine H D Crawford, Caelan E Radford, Ariana G Farrell, Timothy C Yu, William W Hannon, Panpan Zhou, Raiees Andrabi, Dennis R Burton, Lihong Liu, et al.μl of the purified HiFi product to transform 20 μl of 10-beta electrocompetent E. coli cells (NEB, C3020K). We performed 10 electroporation reactions to get a final count of > 2 million CFUs per library and

(14) Resurrecting essential amino acid biosynthesis in mammalian cellseLifeSeptember 27, 2022Trolle J, McBee RM, Kaufman A, Pinglay S, Berger H, German S, Liu L, Shen MJ, Guo X, Martin JA, et al.plasmid was maintained in and purified from NEB 10-beta electrocompetent E. coli (New England Biolabs, C3020K). Lentivirus was packaged by plating 4×106 HEK293T cells on 10 cm2 and incubating cells overnight at

(15) Functional map of SARS-CoV-2 3CL protease reveals tolerant and immutable sitesCell Host & MicrobeAugust 11, 2022Sho Iketani et al.E166V","Ye et al., 2020; this manuscript",N/A 10-beta Electrocompetent E. coli,New England Biolabs,Catalog #C3020K MDS42 E. coli,"Umenhoffer et al., 2010",N/A ,, "Chemicals, peptides, and recombinant proteins","Chemicals,

(16) The SARS-CoV-2 Delta variant induces an antibody response largely focused on class 1 and 2 antibody epitopesbioRxivAugust 10, 2022Allison J. Greaney et al.libraries were electroporated into E. coli (NEB 10-beta electrocompetent cells, New England BioLabs C3020K) and plated at a target bottleneck of 100,000 variants per duplicate library, corresponding to >25 barcodes

(17) A SARS-CoV-2 variant elicits an antibody response with a shifted immunodominance hierarchybioRxivJuly 15, 2022Allison J. Greaney et al.libraries were electroporated into E. coli (NEB 10-beta electrocompetent cells, New England BioLabs C3020K) and plated at a target bottleneck of 50,000 variants per duplicate library, corresponding to >10 barcodes

(18) The SARS-CoV-2 Delta variant induces an antibody response largely focused on class 1 and 2 antibody epitopesPLOS PathogensJune 29, 2022Allison J. Greaney et al.libraries were electroporated into E. coli (NEB 10-beta electrocompetent cells, New England BioLabs C3020K) and plated at a target bottleneck of 100,000 variants per duplicate library, corresponding to >25 barcodes

(19) A SARS-CoV-2 variant elicits an antibody response with a shifted immunodominance hierarchyPLOS PathogensFebruary 8, 2022Allison J. Greaney et al.libraries were electroporated into E. coli (NEB 10-beta electrocompetent cells, New England BioLabs C3020K) and plated at a target bottleneck of 50,000 variants per duplicate library, corresponding to >10 barcodes

(20) Selection for constrained peptides that bind to a single target proteinNature CommunicationsDecember 1, 2021Andrew M. King et al.(Zymo Research, CA). Library assemblies were initially transformed into electrocompetent E. coli NEB 10β (C3020K, NEB, MA). We observed 1.5 × 107 colony forming units (CFU)/mL for lbAMK-103. Total transformants were

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(1)

9/18/2019Transformation of Plasmid into NEB® 10-beta Electrocompetent E. Coli Atul SinghExcellent product to do a plasmid transformation of size >10kb.
(1)

9/12/2018Electrocompetent E. coli for Transforming Large ConstructsDr. Thippeswamy SannasiddappaI have constructed large plasmid having gene interest to clone into recipient bacteria by Gibson assembly method. The constructed plasmid construct was about 15 kb in size and was successfully ... Read Review
(1)

4/2/2018Transformation Of Large Plasmid Constructs Triveni MahendrappaI have designed and constructed large plasmid constructs for expression purposes. Plasmid construct was 23 Kb in size and needed high efficiency electrocompetent cells. Hence I used NEB 10-beta ... Read Review