EcoRI-HF from New England Biolabs

Product Specs
ItemEcoRI-HF
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberR3101S
Quantity10000 units
Concentration20,000 units/ml
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeRestriction Enzyme
Temperature-20 C

Sequence

G⇓AATTC
C⇑CATGG

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Comprehensive identification of onco-exaptation events in bladder cancer cell lines revealed L1PA2-SYT1 as a prognosis-relevant eventiScienceDecember 15, 2023Ziwei Wang, Yidie Ying, Maoyu Wang, Qing Chen, Yi Wang, Xufeng Yu, Wei He, Jing Li, Shuxiong Zeng, Chuanliang XuDouble digestion of pLVX-IRES-Puro plasmids was performed using restriction enzymes EcoRI and BamHI (R3101S & R3136S, New England BioLabs, Massachusetts, US) according to the manufacturer’s instructions. Predicted

(2) Genome sequence and characterization of a novel Pseudomonas putida phage, MiCathSci RepDecember 9, 2023James Jaryenneh, Joseph S. Schoeniger, Catherine M. MageeneydiH20. MiCath and Lambda (NEB, catalog no. N3011S) DNAs were digested with EcoRI-HF (NEB, catalog no. R3101S), BamHI-HF (NEB, catalog no. R3136S), BstXI (NEB, catalog no. R0113S), EcoRV-HF (NEB, catalog no. R3195S),

(3) Protocol for rapidly inducing genome-wide RNA Pol II hyperphosphorylation by selectively disrupting INTAC phosphatase activitySTAR ProtocolsDecember 1, 2023Yu-Xin Ji, Shibin Hu, Fei Xavier Chen,New England Biolabs,Cat.#B6004S BbsI-HF,New England Biolabs,Cat.#R3539S EcoRI-HF,New England Biolabs,Cat.#R3101S Ligation Mix,TaKaRa,Cat.#6023 PEI,Yeasen,Cat.#40816ES Polybrene,Yeasen,Cat.#40804ES86 2× Phanta Max Master Mix

(4) Metastases and treatment-resistant lineages in patient-derived cancer cells of colorectal cancerCommunications BiologyNovember 24, 2023Shiki Fujino, Norikatsu Miyoshi, Aya Ito, Rie Hayashi, Masayoshi Yasui, Chu Matsuda, Masayuki Ohue, Masafumi Horie, Shinichi Yachida, Jun Koseki, et al.digested with restriction enzymes, XhoI (R0146S; New England Biolabs, Beverly, MA, USA) and EcoRI-HF (R3101S; New England Biolabs). The DNA fragment of caspase 9 was extracted from E-Gel CloneWel 0.8% (G6500ST;

(5) Force-Induced Visualization of Nucleic Acid Functions with Single-Nucleotide ResolutionSensors (Basel)September 8, 2023Qiongzheng Hu, Haina Jia, Yuhong Wang, Shoujun XuNEB buffer for DpnII (R0543S, New England BioLabs, Ipswich, MA, USA) and CutSmart Buffer for EcoRI (R3101S, New England BioLabs) and HpaII (R0171S, New England BioLabs), and 41 μL autoclaved ultrapure water

(6) A metabolic map of the DNA damage response identifies PRDX1 in the control of nuclear ROS scavenging and aspartate availabilityMolecular Systems BiologyJuly 11, 2023Amandine Moretton, Savvas Kourtis, Antoni Gañez Zapater, Chiara Calabrò, Maria Lorena Espinar Calvo, Frédéric Fontaine, Evangelia Darai, Etna Abad Cortel, Samuel Block, Laura Pascual‐Reguant, et al.acid,Sigma‐Aldrich/ Merck,A1330000/ A7219 Carboplatin,MedChem Express,HY‐17393 AgeI‐HF,NEB,R3552S EcoRI‐HF,NEB,R3101S BsmBI,NEB,R0580L Lipofectamine 2000 transfection reagent,Thermo Fisher Scientific,11668019 Puromycin dihydrochloride

(7) Tailoring Magnetite-Nanoparticle-Based Nanocarriers for Gene Delivery: Exploiting CRISPRa Potential in Reducing ConditionsNanomaterials (Basel)May 31, 2023David Arango, Javier Cifuentes, Paola Ruiz Puentes, Tatiana Beltran, Amaury Bittar, Camila Ocasión, Carolina Muñoz-Camargo, Natasha I. Bloch, Luis H. Reyes, Juan C. Cruzthe nanobioconjugate to the linearized plasmid), AgeI-HF Enzyme (NEB-R3552S), EcoRI-HF Enzyme (NEB-R3101S), PvuI-HF Enzyme (NEB-R3150S), Q5 High-Fidelity 2X Mastermix (NEB-M0515), Monarch PCR & DNA Cleanup

(8) Creatine kinase brain-type regulates BCAR1 phosphorylation to facilitate DNA damage repairiScienceMay 19, 2023Yang B, Zhang W, Sun L, Lu B, Yin C, Zhang Y, Jiang H.Trans,Life iLab Bio,Cat#C4058L1092 Nhe I,New England Biolabs,Cat#R3131S EcoR I,New England Biolabs,Cat#R3101S BamHI-HF,New England Biolabs,Cat#R3136L TGF-β,Absin,Cat#abs04204 etopside (Cas No.33419-42-0),tansoole,Cat#01158693 TRIzol reagent

(9) DNA rehybridization drives product release from Cas9 ribonucleoprotein to enable multiple-turnover cleavageNucleic Acids ResearchMay 8, 2023Juan Pan, Megumu Mabuchi, Gregory Brett RobbMonarch® PCR & DNA Cleanup Kit (#T1030L), Q5® Hot Start High-Fidelity 2× Master Mix (#M0494L), EcoRI-HF (#R3101S), rSAP (#M0371S), T4 DNA ligase (#M0202S), NEB® 10-beta Competent Escherichia coli (High Efficiency)

(10) The DNA damage response in advanced ovarian cancer: functional analysis combined with machine learning identifies signatures that correlate with chemotherapy sensitivity and patient outcomeBritish journal of cancerMay 1, 2023Walker TDJ, Faraahi ZF, Price MJ, Hawarden A, Waddell CA, Russell B, Jones DM, McCormick A, Gavrielides N, Tyagi S, et al.(200236; Agilent) were transformed with reconstituted pCMV6-AC-GFP plasmid (ps100010; Origene). EcoRI-HF (R3101S), SacI-HF (R3156S), KpnI-HF (R3142S), PmeI (R0560S), and Quick CIP (M0525) (New England Biolabs) were

(11) Probing Oxidant Effects on Superoxide Dismutase 1 Oligomeric States in Live Cells Using Single-Molecule Fluorescence AnisotropyChemical & Biomedical ImagingApril 24, 2023Huanhuan Chen, Tai-Yen Chenamplified mEos4b was then inserted into the monomeric mEos4b plasmid between cutting sites EcoRI (NEB R3101S) and BamHI to generate the dimeric mEos4b construct. The linker sequence between two mEos4b: TTGGATCCACCGGTCGCCACC.

(12) An RNA-based system to study hepatitis B virus replication and evaluate antiviralsScience advancesApril 14, 2023Yu Y, Schneider WM, Kass MA, Michailidis E, Acevedo A, Pamplona Mosimann AL, Bordignon J, Koenig A, Livingston CM, van Gijzel H, et al.Zymo Clean and Concentrator-5 (Zymo, catalog no. D4004), digested with Eco RI–HF (NEB, catalog no. R3101S) and Sac II (NEB, catalog no. R0157S), gel-extracted with agarose dissolving buffer (ADB) (Zymo, catalog

(13) Epitranscriptomic regulation of cortical neurogenesis via Mettl8-dependent mitochondrial tRNA m(3)C modificationCell stem cellMarch 2, 2023Zhang F, Yoon K, Zhang DY, Kim NS, Ming GL, Song H.Phosphate-Buffered Saline (DPBS),Corning,Cat#14190250 EcoRI-HF restriction enzyme,New England Biolabs Inc,Cat#R3101S EdU,Thermo Fisher Scientific,Cat#E10187 EZ-Tn5 Transposase,Lucigen,Cat#TNP92110 Fast Green,VWR Chemicals,Cat#E772-25G Fast

(14) LncGMDS-AS1 promotes the tumorigenesis of colorectal cancer through HuR-STAT3/Wnt axisCell death & diseaseFebruary 27, 2023Ye D, Liu H, Zhao G, Chen A, Jiang Y, Hu Y, Liu D, Xie N, Liang W, Chen X, et al.following reagents were used: NEBuffer 2 (B7002S, New England Biolabs), Age I (R3552S, NEB), EcoR I (R3101S, NEB), BamH I (FD-0054, Thermo Fisher), DNA ligation kit reagents (6022, TaKaRa), and TransScript First-Strand cDNA Synthesis SuperMix

(15) Comparison of the structure-function properties of wild-type human apoA-V and a C-terminal truncation associated with elevated plasma triglyceridesmedRxivFebruary 26, 2023Sylvia Stankov, Cecilia Vitali, Joseph Park, David Nguyen, Leland Mayne, S. Walter Englander, Michael G. Regeneron Genetics Center, Michael G. Vujkovic, Nicholas J. Vujkovic, Nicholas J. Phillips, et al.R: 5’-ATTATAGAATTCTCAGGGGTCCCCCAGATGGCTGTG-3’, digested with Xhol (NEB, R0146S) and EcoRI-HF (NEB, R3101S) then sub-cloned into dephosphorylated Xhol and EcoRI-HF digested pcDNA3.1/myc-His(−)A expression plasmid

(16) Do Amino Acid Antiporters Have Asymmetric Substrate Specificity?BiomoleculesFebruary 6, 2023Gregory Gauthier-Coles, Stephen J. Fairweather, Angelika Bröer, Stefan Bröerof the EcoRI restriction site in the MCS using the EcoRI-HF restriction enzyme (New England BioLabs; R3101S) according to the vendor’s instructions. Ligation of the insert into pGHJ was conducted using the Gibson assembly cloning kit

(17) Pathogen evasion of social immunityNature Ecology & EvolutionFebruary 2, 2023Miriam Stock, Barbara Milutinović, Michaela Hoenigsberger, Anna V. Grasse, Florian Wiesenhofer, Niklas Kampleitner, Madhumitha Narasimhan, Thomas Schmitt, Sylvia Cremerbuffer AE. The genomic DNA was digested using EcoRI-HF and HindIII-HF enzymes (both New England Biolabs, R3101S and R3104S) within the 20 μl 1x ddPCR reaction, comprising: 10 μl 2× ddPCR Supermix for probes (Bio-Rad,

(18) Precursor-derived in-water peracetic acid impacts on broiler performance, gut microbiota, and antimicrobial resistance genesPoultry ScienceFebruary 1, 2023Salvatore Galgano, Leah Conway, Francesco Di Maggio, Kathryn Farthing, Nikki Dalby, Adrian Fellows, Jos G.M. Houdijkkit as per manufacturer instructions (27104, QIAGEN,). Insert presence was verified both by EcoRI (R3101S, New England BioLabs, Ipswich, MA) restriction enzyme digestion and by Sanger sequencing (DNA Sequencing

(19) Improved ANAP incorporation and VCF analysis reveals details of P2X7 current facilitation and a limited conformational interplay between ATP binding and the intracellular ballast domaineLifeJanuary 4, 2023Anna Durner, Ellis Durner, Annette NicketgaCCCAAAACAAAAACGGAATATGCAAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAATTCTAGAGCGGCCGCagagtcgacctgcagg","pNKS2, Gloor et al., 1995",, "Peptide, recombinant protein",EcoRI-HF,New England Biolabs GmbH,CAT#R3101S, "Peptide, recombinant protein",NotI-HF,New England Biolabs GmbH,CAT#R3189S, Commercial assay or kit,Gibson Assembly Master Mix

(20) An ultra-sensitive method to detect mutations in human RAS templatesSmall GTPasesDecember 31, 2022Siqi Li, Christopher M. Counteramplicon was cloned into pcDNA3.1 (ThermoFisher Scientific V79020) using restriction enzymes EcoRI (NEB R3101S) and XhoI (NEB R0146S). MDS assay and library purification The series of plasmids containing KRAS

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10/1/2015High Fidelity Restriction Enzyme for Cloning Prabhakaran KumarI used this EcoRI-HF restriction enzyme for one way (Single enzyme digestion) cloning of my gene of interest in to plasmid vector. Subsequently I used this enzyme for colony screening also. This ... Read Review

EcoRI-HF

EcoRI-HF from New England Biolabs

Description

Sequence

New England Biolabs

Citations (143)

Reviews

EcoRI-HF from New England Biolabs

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