MojoSort(TM) Streptavidin Nanobeads from BioLegend

Product Specs
ItemMojoSort(TM) Streptavidin Nanobeads
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number480016
Quantity1 mL
ApplicationsCell Sep - Pos, Cell Sep - Neg
FormatAqueous solution containing BSA and 0.05% sodium azide
TypeResins
ModeAffinity Purification
Conjugate/Tag/LabelMOJO

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Lymph node medulla regulates the spatiotemporal unfolding of resident dendritic cell networksImmunityAugust 8, 2023Ugur M, Labios RJ, Fenton C, Knöpper K, Jobin K, Imdahl F, Golda G, Hoh K, Grafen A, Kaisho T, et al.4260145182276 MojoSort™ Buffer (5X),BioLegend,Cat# 480017 MojoSort™ Streptavidin Nanobeads,BioLegend,Cat# 480016 Normal Mouse Serum,Invitrogen,Cat# 10410 OCT freezing media,Sakura Finetek,Cat# 12351753 Optiprep™,Sigma

(2) A Th1-like CD4+ T-cell Cluster That Predicts Disease-free Survival in Early-stage Lung CancerCancer Research CommunicationsJuly 19, 2023Akitoshi Yanagihara, Satoshi Yamasaki, Kosuke Hashimoto, Ryo Taguchi, Tetsuya Umesaki, Hisao Imai, Kyoichi Kaira, Hiroyuki Nitanda, Hirozo Sakaguchi, Hironori Ishida, et al.to the manufacturer's instructions. Biotin anti-human CD235ab antibody and streptavidin nanobeads (480016, BioLegend) were used to remove erythrocytes, according to the manufacturer's instructions. Mass Cytometry The

(3) A microcarrier-based protocol for scalable generation and purification of human induced pluripotent stem cell-derived neurons and astrocytesSTAR ProtocolsSeptember 16, 2022Jacob Knittel, Gayathri Srinivasan, Carlye Frisch, Nicholas Brookhouser, Sreedevi Raman, Albert Essuman, David A. BrafmanP6866 Sartorius Quintix213-1S Scale,VWR,Cat#: 611-2646 Streptavidin Magnetic Nanobeads,BioLegend,Cat#: 480016 μ-Slide 8 Well Glass Bottom: # 1.5H,ibidi,Cat# 80827 VWR Dura Shaker,VWR,Cat#: 10159-960 Water bath,VWR,N/A Weigh

(4) Detection of exogenous DNA uptake by murine dendritic cellsSTAR protocolsSeptember 16, 2022Celias DP, de Mingo Pulido Á, Ruffell B.LLC,https://www.flowjo.com/ Prism,GraphPad,https://www.graphpad.com/scientific-software/prism/ ,, Other,Other,Other ,, MojoSort Streptavidin Nanobeads,BioLegend,480016 EasySep™ Magnet,STEMCELL,18000 "Corning™ Costar™ 96-Well, Cell Culture-Treated, U-Shaped-Bottom Microplate",Fisher

(5) Liver group 2 innate lymphoid cells regulate blood glucose levels through IL-13 signaling and suppression of gluconeogenesisNature communicationsSeptember 15, 2022Fujimoto M, Yokoyama M, Kiuchi M, Hosokawa H, Nakayama A, Hashimoto N, Sakuma I, Nagano H, Yamagata K, Kudo F, et al.(Miltenyi) with an anti-mouse lineage panel (BioLegend, 133307) and streptavidin nanobeads (BioLegend, 480016). Then, single-cell suspensions were stained using blocking and staining antibodies in the dark at 4

(6) HIC2 controls developmental hemoglobin switching by repressing BCL11A transcriptionNature geneticsSeptember 1, 2022Huang P, Peslak SA, Ren R, Khandros E, Qin K, Keller CA, Giardine B, Bell HW, Lan X, Sharma M, et al.R17217; 1:200). CD71− cells were isolated using streptavidin magnetic beads (BioLegend, catalog no. 480016). Cells were then resuspended in 100 μl of HEMOX buffer supplemented with 20 mM glucose and 0.32% bovine

(7) Combined analysis of T cell activation and T cell-mediated cytotoxicity by imaging cytometryJournal of Immunological MethodsJuly 1, 2022Monica K. Chanda, Claire E. Shudde, Taylor L. Piper, Yating Zheng, Adam H. Courtney(PE) (53–6.7) were obtained from Biolegend. Streptavidin nanospheres were obtained from Biolegend (#480016). Biotinylated antibodies used for negative selection cocktail were obtained from Biolegend: Biotin

(8) Indoleamine 2,3-dioxygenase 1 activation in mature cDC1 promotes tolerogenic education of inflammatory cDC2 via metabolic communicationImmunityJune 14, 2022Gargaro M, Scalisi G, Manni G, Briseño CG, Bagadia P, Durai V, Theisen DJ, Kim S, Castelli M, Xu CA, et al.negative selection beads,Invitrogen™,Cat#: MSNB-6002 MojoSort™ Streptavidin Nanobeads,Biolegend,Cat#: 480016 Qdot™605 Streptavidin conjugate,Invitrogen™,Cat#: Q10101MP APC or PeCy7 Streptavidin,BioLegend,"Cat#:

(9) Ena/VASP Protein-Mediated Actin Polymerization Contributes to Naïve CD8+ T Cell Activation and Expansion by Promoting T Cell–APC Interactions In VivoFrontiers in ImmunologyJune 9, 2022Monique M. Waldman, Jeremy T. Rahkola, Ashton L. Sigler, Jeffrey W. Chung, Benjamin A. S. Willett, Ross M. Kedl, Rachel S. Friedman, Jordan Jacobelliselection using the following antibodies (see table below) and streptavidin beads (Biolegend, Cat. 480016). Finally, the remaining splenocytes were stained for flow cytometry analysis as above. For isolation

(10) Zinc finger protein Zfp335 controls early T-cell development and survival through β-selection-dependent and -independent mechanismseLifeFebruary 3, 2022Wang X, Jiao A, Sun L, Li W, Yang B, Su Y, Ding R, Zhang C, Liu H, Yang X, et al.

(11) Intra‐heterogeneity in transcription and chemoresistant property of leukemia‐initiating cells in murine Setd2 ^−/− acute myeloid leukemiaCancer CommunicationsSeptember 1, 2021Jiachun Song et al.MojoSort™ Buffer (480017, BioLegend) separately. Cells were then labeled with Streptavidin Nanobeads (480016, BioLegend) on ice and washed again 15 min later. Cell pellets were resuspended in MojoSort™ Buffer,

(12) The inhibitory receptor TIM-3 limits activation of the cGAS-STING pathway in intra-tumoral dendritic cells by suppressing extracellular DNA uptakeImmunityJune 8, 2021de Mingo Pulido Á, Hänggi K, Celias DP, Gardner A, Li J, Batista-Bittencourt B, Mohamed E, Trillo-Tinoco J, Osunmakinde O, Peña R, et al.Particles (10.0–14.0 μm),Spherotech,Cat# HUAMP-100–4 MojoSort Streptavidin Nanobeads,BioLegend,Cat# 480016

(13) UTX promotes CD8⁺ T cell-mediated antiviral defenses but reduces T cell durabilityCell reportsApril 13, 2021Mitchell JE, Lund MM, Starmer J, Ge K, Magnuson T, Shpargel KB, Whitmire JK.Mouse CD8 T Cell Isolation Kit,Biolegend,Cat#480008 MojoSort Streptavidin Nanobeads,Biolegend,Cat#480016 UltraComp eBeads Compensation beads,ThermoFisher Scientific,Cat#01-2222-41 Deposited data,Deposited

(14) Frontline Science: CD40 signaling restricts RNA virus replication in Mϕs, leading to rapid innate immune control of acute virus infectionJournal of leukocyte biologyFebruary 1, 2021Rogers KJ, Shtanko O, Stunz LL, Mallinger LN, Arkee T, Schmidt ME, Bohan D, Brunton B, White JM, Varga SM, et al.and CD19 (1D3). Separation was performed using MojoSort Streptavadin Nanobeads (Biolegend, catalog #480016) in accordance to manufacturer’s protocol. Validation of depletion was performed using antibodies described

(15) High-resolution targeted 3C interrogation of cis-regulatory element organization at genome-wide scaleNature communicationsJanuary 22, 2021Downes DJ, Beagrie RA, Gosden ME, Telenius J, Carpenter SJ, Nussbaum L, De Ornellas S, Sergeant M, Eijsbouts CQ, Schwessinger R, et al.(BD 553672) for 30 min, before magnetic depletion using streptavidin nanobeads (BioLegend Mojosort 480016) following the manufacturer’s instructions. Cells were then incubated for 45 min with 0.5 μg/mL APC-conjugated

(16) In Situ Maturation and Tissue Adaptation of Type 2 Innate Lymphoid Cell ProgenitorsImmunityOctober 13, 2020Zeis P, Lian M, Fan X, Herman JS, Hernandez DC, Gentek R, Elias S, Symowski C, Knöpper K, Peltokangas N, et al.depletion of Lin+ cells with anti-biotin Microbeads (Miltenyi) or (MojoSort Streptavidin Nanobeads, 480016, Biolegend) according to the manufacturer’s recommendations before sorting. Staining and enrichment

(17) Regulatory B Lymphocytes Colonize the Respiratory Tract of Neonatal Mice and Modulate Immune Responses of Alveolar Macrophages to RSV Infection in IL-10-Dependant MannerVirusesJuly 29, 2020Laubreton D, Drajac C, Eléouët JF, Rameix-Welti MA, Lo-Man R, Riffault S, Descamps D.biotinylated antibody (Biolegend, San Diego, CA, USA, #115504) and streptavidin-magnetic beads (Biolegend #480016) on magnetic MS MACS column (Miltenyi Biotec, Paris, France). B-cell subsets were then FACS-sorted on

(18) Umbilical cord blood-derived ILC1-like cells constitute a novel precursor for mature KIR⁺NKG2A^- NK cellseLifeJuly 13, 2020Bennstein SB, Weinhold S, Manser AR, Scherenschlich N, Noll A, Raba K, Kögler G, Walter L, Uhrberg M.740722,Human T Helper Cytokine Panel Commercial assay or kit,MojoSortStreptavidin Nanobeads,Biolegend,Cat: 480016,50µl/10x107 Commercial assay or kit,Illumina Tagment DNA Enzyme and Buffer Small Kit,Illumina,Cat:

(19) Dynamics of the 4D genome during in vivo lineage specification and differentiationNature communicationsJune 1, 2020Oudelaar AM, Beagrie RA, Gosden M, de Ornellas S, Georgiades E, Kerry J, Hidalgo D, Carrelha J, Shivalingam A, El-Sagheer AH, et al.(BD 553672) for 30 min, before magnetic depletion using streptavidin nanobeads (BioLegend Mojosort 480016) following the manufacturer’s instructions. Cells were then incubated with 0.5 μg/ml APC-conjugated

(20) Tim-4 Inhibits NLRP3 Inflammasome via the LKB1/AMPKα Pathway in MacrophagesJournal of immunology (Baltimore, Md. : 1950)August 15, 2019Liu W, Bai F, Wang H, Liang Y, Du X, Liu C, Cai D, Peng J, Zhong G, Liang X, et al.suspension of hepatic mononuclear cells by Streptavidin Nanobeads combined with biotin-conjugated F4/80 Abs (480016; BioLegend). Detailed experimental procedures were carried out according to the manufacturer’s protocols.

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9/18/2018Good Way to Purify CD4 or CD8 T CellsDr. Minghua ZhuUsing Streptavidin Nanobead, CD4 or CD8 cells can be selectively purified from splenocytes in one hour, with good purity and yield.

MojoSort(TM) Streptavidin Nanobeads from BioLegend

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MojoSort(TM) Streptavidin Nanobeads from BioLegend

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