GoTaq G2 Hot Start Green Master Mix from Promega

Product Specs
ItemGoTaq G2 Hot Start Green Master Mix
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberM7422
Quantity100 reactions

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Discovery and multimerization of cross-reactive single-domain antibodies against SARS-like viruses to enhance potency and address emerging SARS-CoV-2 variantsSci RepAugust 22, 2023Scott A. Hollingsworth, Cameron L. Noland, Karin Vroom, Anasuya Saha, Miranda Sam, Qinshan Gao, Haihong Zhou, David U. Grandy, Sujata Singh, Zhiyun Wen, et al.(TSO) for 5’RACE. A first round PCR reaction was then performed using GoTaq polymerase (Promega, Cat # M7422), a llama heavy chain only gene specific constant region reverse primer, and a TSO-compatible forward

(2) PMS1 as a target for splice modulation to prevent somatic CAG repeat expansion in Huntington's diseasebioRxivJuly 30, 2023Zachariah L. McLean, Dadi Gao, Kevin Correia, Jennie C. L. Roy, Shota Shibata, Iris N. Farnum, Zoe Valdepenas-Mellor, Manasa Rapuru, Elisabetta Morini, Jayla Ruliera, et al.exons flanking the pseudoexon (Supplementary table 1). We used GoTaq G2 Hot Start PCR kit (Promega, M7423) with the following conditions: initial denaturation 94 °C (2 min), 40 cycles of 94 °C (30 s), 60 °C

(3) A Rab33b missense mouse model for Smith-McCort dysplasia shows bone resorption defects and altered protein glycosylationFrontiers in GeneticsJune 8, 2023Milena Dimori, Irina D. Pokrovskaya, Shijie Liu, John T. Sherrill, Horacio Gomez-Acevedo, Qiang Fu, Brian Storrie, Vladimir V. Lupashin, Roy Morellomice for the study. PCR genotyping was performed with the GoTaq G2 Hot Start Polymerase reagent (cat# M7423 Promega) using a Master Cycler thermocycler (Eppendorf). An example gel image of the genotyping results

(4) Suspension culture improves iPSC expansion and pluripotency phenotypeStem Cell Research & TherapyJune 6, 2023Nerea Cuesta-Gomez, Kevin Verhoeff, Nidheesh Dadheech, Tiffany Dang, Ila Tewari Jasra, Mario Bermudez de Leon, Rena Pawlick, Braulio Marfil-Garza, Perveen Anwar, Haide Razavy, et al.as described in Additional file 1: Table S2. GoTaq G2 Hot Start Colorless Master Mix (Promega, cat. M7422) was used alongside forward and reverse primers as described in Additional file 1: Table S3. Samples

(5) DNA Methyltransferase Genes Are Associated with Oral Mucositis and Creatinine Levels in Oncopediatric PatientsGenes (Basel)May 24, 2023Beatriz Fernandes de Souza, José Maria Chagas Viana Filho, José Nunes de Queiroz Neto, Marina de Castro de Castro Coêlho, Ana Maria Gondim Valença, Darlene Camati Persuhn, Naila Francis Paulo de Oliveirareactions containing 10 μL of GoTaq® G2 Hot Start Green Master Mix (Promega Corporation, Madison, WI, USA; M7423), forward and reverse primers, 1 µL of DNA, and nuclease-free water. After amplification, enzymatic

(6) An Infectious Bursal Disease Virus (IBDV) Reverse Genetics Rescue System and Neutralization Assay in Chicken B CellsCurrent ProtocolsJanuary 9, 2023Vishwanatha R. A. P. Reddy, Salik Nazki, Amin Asfor, Andrew J. Broadbent20210105)10 mM 4dNTP mix (dATP, dCTP, dGTP, dTTP)GoTaq G2® Hot Start Master Mix (Promega, cat. no. M7422)4% (v/v) paraformaldehyde (Santa Cruz Biotechnology, cat. no. sc‐281692)Triton™ X‐100 (Scientific Laboratory

(7) Selective ablation of P53 in pancreatic beta cells fails to ameliorate glucose metabolism in genetic, dietary and pharmacological models of diabetes mellitusMolecular metabolismJanuary 1, 2023Uhlemeyer C, Müller N, Rieck M, Kuboth J, Schlegel C, Grieß K, Dorweiler TF, Heiduschka S, Eckel J, Roden M, et al.using standard protocols using appropriate primers (Table S2) and GoTaq G2 Hot Start Green Master Mix (#M7423, Promega). 2.3 Body weight, blood glucose levels, glucose and insulin tolerance tests Body weight

(8) A genetically engineered, stem-cell-derived cellular vaccineCell reports. MedicineDecember 20, 2022Cooper A, Sidaway A, Chandrashekar A, Latta E, Chakraborty K, Yu J, McMahan K, Giffin V, Manickam C, Kroll K, et al.07920_C CryoStor-CS10,StemCell Technologies,Cat# 07930_C Proteinase K,Promega,Cat# MC5005 GoTaq G2 PCR master mix,Promega,Cat# M7422 Human Heat Inactivated AB Serum,Sigma,Cat# H3667 RIPA buffer,ThermoFisher,Cat# 89900 LDS Sample Buffer,ThermoFisher,Cat#

(9) Potent Inactivation of Human Respiratory Viruses Including SARS-CoV-2 by a Photoactivated Self-Cleaning Regenerative Antiviral CoatingACS Applied Materials & InterfacesSeptember 14, 2022Udit Kumar, Candace R. Fox, Elayaraja Kolanthai, Craig J. Neal, Kritika Kedarinath, Yifei Fu, Erik Marcelo, Balaashwin Babu, Griffith D. Parks, Sudipta Seal5′-GCAGTGGAGGCAACACTTTG-3′. RT-PCR was performed using GoTaq G2 Hot Start Green Mater Mix (catalog number M7422, Promega, Madison, WI, USA) according to the manufacturer’s instructions. PCR products were run on a

(10) Revision of the family Milacidae from Switzerland (Mollusca, Eupulmonata, Parmacelloidea)ZooKeysAugust 9, 2022Vivianne M. Schallenberg, René Heim, Ulrich E. Schneppat, Peter Müller, Jörg Rüetschi, Eike NeubertFor the COIPCR mixture 3 µL of DNA template in 12.5 µL GoTaq G2 HotStart Green Master Mix (Promega M7423), 7.5 µL ddH2O, 1 µL of the established Folmer primers LCO1490 and HCO2198 (Folmer et al. 1994) were

(11) Skeletal Stem/Progenitor Cells in Periosteum and Skeletal Muscle Share a Common Molecular Response to Bone InjuryJournal of Bone and Mineral ResearchAugust 1, 2022Anais Julien, Simon Perrin, Ester MartÃnezâ€SarrÃ , Anuya Kanagalingam, Caroline Carvalho, Marine Luka, MickaÃ«l MÃ©nager, CÃ©line Colnotpolymerase chain reaction (PCR) was performed with GoTaq G2 Hot Start Green Master Mix (Promega, San Luis Obispo, CA, USA; ref M7423) using the following primers: Fx2: 5′‐GCA GCT GCT GCT GCA GCC TCC‐3′, Fx4: 5′‐TGG CTA CAA TTT GTC TCA

(12) On the verge of extinction – revision of a highly endangered Swiss alpine snail with description of a new genus, Raeticella gen. nov. (Gastropoda, Eupulmonata, Hygromiidae)ZooKeysJune 8, 2022Jeannette Kneubühler, Markus Baggenstos, Eike NeubertrRNA+ITS2) were investigated. PCR mixtures consisted of 12.5 µl GoTaq G2 HotStart Green Master Mix (Promega M7423), 4.5 µl ddH2O, 2 µl forward and reverse primer each, and 4 µl DNA template. The primer pairs implemented

(13) The mammalian trafficking chaperone protein UNC93B1 maintains the ER calcium sensor STIM1 in a dimeric state primed for translocation to the ER cortexJournal of Biological ChemistryMarch 1, 2022Wen-An Wang, Nicolas Demaurexno.: G1N70-1KT; Sigma), and PCR was carried out with GoTaq G2 Hot Start Green Master Mix (catalog no.: M7422; Promega). PCR product bands were detected following separation on a 1% agarose gel and isolated by

(14) Recursive splicing is a rare event in the mouse brainPLOS ONEJanuary 28, 2022Sohyun Moon, Ying-Tao Zhaosynthesize the cDNA. The RT-PCR experiments were conducted using the GoTaq G2 Hot Start Master mix (Promega #M7422). The PCR primers were listed in the S5 Fig. The RT-PCR were conducted using the following setting:

(15) High throughput screening identifies SOX2 as a super pioneer factor that inhibits DNA methylation maintenance at its binding sitesNature CommunicationsDecember 1, 2021Ludovica Vanzan, Hadrien Soldati, Victor Ythier, Santosh Anand, Simon M. G. Braun, Nicole Francis, Rabih MurrSupplementary Table 5. The PCR steps were done using the Promega Go-TaqG2 Hot Start Green Mastermix (Promega, M7423), set up according to manufacturer’s protocol, and all the purification steps using the Qiagen GenRead

(16) Mds1CreERT2, an inducible Cre allele specific to adult-repopulating hematopoietic stem cellsCell ReportsAugust 17, 2021Yi Zhang, Kathleen E. McGrath, Edward Ayoub, Paul D. Kingsley, Hongbo Yu, Kate Fegan, Kelly A. McGlynn, Sarah Rudzinskas, James Palis, Archibald S. PerkinsCommercial Assays SuperScript IV One-Step RT-PCR System,Thermofisher,Cat# 12594025 GoTaq,Promega,Cat# M7422 Experimental Models: Organisms/Strains,Experimental Models: Organisms/Strains,Experimental Models:

(17) The bacterial toxin ExoU requires a host trafficking chaperone for transportation and to induce necrosisNature communicationsJune 29, 2021Deruelle V, Bouillot S, Job V, Taillebourg E, Fauvarque MO, Attrée I, Huber P.of amplified gRNAs. The PCR conditions for 50 µL were 25 µl of GoTaq G2 Hot Start Green Master Mix (#M7423, Promega), 0.5 µM of each primer (Supplementary Table 3) and 2.5 µg of gDNA. Three PCR reactions were

(18) R-locus for roaned coat is associated with a tandem duplication in an intronic region of USH2A in dogs and also contributes to Dalmatian spottingPLOS ONEMarch 23, 2021Takeshi Kawakami, Meghan K. Jensen, Andrea Slavney, Petra E. Deane, Ausra Milano, Vandana Raghavan, Brett Ford, Erin T. Chu, Aaron J. Sams, Adam R. Boykoprimer pair). All PCR reactions were performed using Go Taq G2 Hot Start Green Master Mix (Promega M7422) in a total volume of 25 uL following the manufacturer’s protocol. The following cycling parameters

(19) Genomic Instability Profiles at the Single Cell Level in Mouse Colorectal Cancers of Defined GenotypesCancers (Basel)March 12, 2021Vasilis S. Dionellis, Maxim Norkin, Angeliki Karamichali, Giacomo G. Rossetti, Joerg Huelsken, Paloma Ordonez-Moran, Thanos D. Halazonetiswere diluted 10 times with water and subjected to PCR amplification using GoTaq Hot Start Polymerase (M7423, Promega). The following primers were used for genotyping: for the Cre allele: Cre_Fw (50 CACCAGCCAGCTATCAACTCG-30

(20) New findings in oligogenic inheritance of congenital hypogonadotropic hypogonadismArchives of Medical ScienceSeptember 18, 2020Agnieszka Gach et al.Primer3 Input (version 0.4.0) program. After PCR amplification with PCR Master Mix (Promega, Cat. No. M7423), products were cleaned and sequenced using BigDye Terminator v3.1 Cycle Sequencing Kit (ThermoFisher Scientific

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3/15/2018Great PCR Enzyme Brian ORourkePre-mixed master mix. All you need to do is add DNA and primers. Gives a quick readout with high fidelity.