TGX™ FastCast™ Acrylamide Kit, 10% from Bio-Rad

Product Specs
ItemTGX™ FastCast™ Acrylamide Kit, 10%
CompanyBio-Rad
Price Pricing Info Supplier Page View Company Product Page
Catalog Number1610173
Brand/TrademarkLSG LSD

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) Intratumoral androgen biosynthesis associated with 3β-hydroxysteroid dehydrogenase 1 promotes resistance to radiotherapy in prostate cancerJournal of Clinical InvestigationNovember 15, 2023Shinjini Ganguly, Zaeem Lone, Andrew Muskara, Jarrell Imamura, Aimalie Hardaway, Mona Patel, Mike Berk, Timothy D. Smile, Elai Davicioni, Kevin L. Stephans, et al.at 95°C for 10 minutes. Samples of 15 to 20 μg were electrophoresed on polyacrylamide gels (Bio-Rad, 1610173) using the Sub-Cell GT System (Bio-Rad, 1704486) and transferred using TurboBlot (Bio-Rad, 1704150)

(2) Ammonium tetrathiomolybdate relieves oxidative stress in cisplatin-induced acute kidney injury via NRF2 signaling pathwayCell death discoveryJuly 25, 2023Qi H, Shi H, Yan M, Zhao L, Yin Y, Tan X, Qi H, Li H, Weng K, Tang Y, et al.determine the concentration. An equivalent quantity of protein was separated on a 7.5 ~ 12% SDS-PAGE gel (1610173, Bio-Rad) and transferred to PVDF membranes (ISEQ00010, Millipore). The membranes were blocked and probed

(3) Protocol to evaluate the inflammatory response in human macrophages induced by SARS-CoV-2 spike-pseudotyped VLPsSTAR ProtocolsMarch 1, 2023Maggie Jing Ouyang, Zhujun Ao, Titus A. Olukitibi, Xiao-Jian YaoU/mL),Gibco,15140122 Opti-MEM reduced serum,Gibco,31985062 "TGX FastCast Acrylamide Kit, 10%",Bio-Rad,1610173 4× Laemmli Sample Buffer,Bio-Rad,1610747 TMB Chromogen solution for ELISA,Invitrogen,002023 ECL Western Blotting Substrate

(4) Epilepsy gene prickle ensures neuropil glial ensheathment through regulating cell adhesion moleculesiScienceJanuary 20, 2023Yanbo Chen, Tong-Tong Liu, Mengxia Niu, Xiaoting Li, Xinwei Wang, Tong Liu, Yan Li,Vector Laboratories,H-1000 Protein G PLUS-Agarose,Santa Cruz,sc-2002 "TGX™ FastCast™ Acrylamide Kit, 10%",Bio-Rad,1610173 "TGX™ FastCast™ Acrylamide Kit, 12%",Bio-Rad,1610175 "Paraformaldehyde Aqueous Solution, 8%",EMS,157-8 "DMEM, high glucose, GlutaMAX

(5) Follicular fluid-derived exosomal miR-143-3p/miR-155-5p regulate follicular dysplasia by modulating glycolysis in granulosa cells in polycystic ovary syndromeCell Communication and SignalingDecember 1, 2022Jianping Cao, Peng Huo, Kuiqing Cui, Huimei Wei, Junna Cao, Jinyuan Wang, Qingyou Liu, Xiaocan Lei, Shun ZhangDenatured proteins were separated by 10% sodium dodecyl sulphate–polyacrylamide gel electrophoresis (#1610173, TGX FastCast Acrylamide Kit, Bio-Rad, Hercules, USA) and then transferred to polyvinylidene membranes.

(6) Inducing respiratory complex I impairment elicits an increase in PGC1α in ovarian cancerScientific ReportsDecember 1, 2022Monica De Luise et al.were used. Samples were separated by SDS–PAGE using a TGXTM FastCastTM Acrylamide kit, 10% (Bio–Rad #1610173), and transferred onto nitrocellulose membranes using a Turbo-pack system (Bio–Rad #1704159SP5). Membranes

(7) Beneficial effects of SS-31 peptide on cardiac mitochondrial dysfunction in tafazzin knockdown miceScientific reportsNovember 18, 2022Russo S, De Rasmo D, Signorile A, Corcelli A, Lobasso S.min at 37 °C, samples were loaded into 10% polyacrylamide gels (BioRad TGX FastCast Acrylamide Kit, #1610173), electrophoresed and then transferred to a nitrocellulose membrane. The membranes were blocked in 5%

(8) MicroRNA and Metabolic Profiling of a Primary Ovarian Neuroendocrine Carcinoma Pulmonary-Type Reveals a High Degree of Similarity with Small Cell Lung CancerNon-coding RNASeptember 25, 2022Miglietta S, Girolimetti G, Marchio L, Sollazzo M, Laprovitera N, Coluccelli S, De Biase D, De Leo A, Santini D, Kurelac I, et al.and crude mitochondria were separated by SDS-PAGE using a TGX Fast-Cast-TM Acrylamide kit (Bio-Rad #1610173). Membranes were blocked at 37 °C for 30 min in TBS-Tween/BSA 5% and incubated with primary antibodies

(9) ACE2‐enriched extracellular vesicles enhance infectivity of live SARS‐CoV‐2 virusJournal of Extracellular VesiclesMay 18, 2022Sze Keong Tey, Hoiyan Lam, Samuel Wan Ki Wong, Hanjun Zhao, Kelvin Kai‐Wang To, Judy Wai Ping Yam–polyacrylamide gel electrophoresis (SDS‐PAGE) using 7.5% or 10% gel made by TGX FastCast Acrylamide Kit (Bio‐Rad Corporation, Cat# 1610173). After electrophoresis, proteins were transferred to PVDF membranes (Cytiva, Cat# GE10600023) using

(10) Sexual dimorphism: increased sterol excretion leads to hypocholesterolaemia in female hyperbilirubinaemic Gunn ratsThe Journal of PhysiologyApril 1, 2022Josif Vidimce, Johara Pillay, Onne Ronda, Ai‐Ching Boon, Evan Pennell, Kevin J. Ashton, Theo H. van Dijk, Karl‐Heinz Wagner, Henkjan J. Verkade, Andrew C. Bulmer(10–30 μg) were separated on SDS‐PAGE (7.5, 10 or 12%) using TGX FastCast acrylamide kits (#1610171, #1610173 and #1610175, Bio‐Rad). After electrophoresis, proteins were transferred onto 0.45 μm polyvinylidene

(11) Subcutaneous adipose tissue sclerostin is reduced and Wnt signaling is enhanced following 4-weeks of sprint interval training in young men with obesityPhysiological reportsMarch 1, 2022Kurgan N, Islam H, Matusiak JBL, Baranowski BJ, Stoikos J, Fajardo VA, MacPherson REK, Gurd BJ, Klentrou P.brown adipose tissue was loaded as a positive control. Proteins were resolved on 10% TGX fast cast gels (CAT# 1610173, Bio‐Rad) for 30 min at 250 V and then were then semi‐dry transferred onto a polyvinylidene difluoride

(12) SPOCK1 Promotes the Development of Hepatocellular CarcinomaFrontiers in OncologyFebruary 3, 2022Lóránd Váncza et al.sodium dodecyl sulfate (SDS)–polyacrylamide gel electrophoresis (TGX FastCast Acrylamide Kit, 10%, #1610173, Bio-Rad, CA, USA) and transferred to polyvinylidene difluoride (PVDF) membrane (IPVH00010, Merck Millipore,

(13) β-catenin links cell seeding density to global gene expression during mouse embryonic stem cell differentiationiScienceJanuary 21, 2022LeBlanc L, Kim M, Kambhampati A, Son AJ, Ramirez N, Kim J.proteins","Chemicals, peptides, and recombinant proteins" ,, TGX FastCast Acrylamide,Bio-Rad,Cat#1610173 Spectra™ Multicolor Broad Range Protein Ladder,Fisher Scientific,Cat#PI26634 Ponceau S,VWR,Cat#K793-500ML Lipofectamine™ 3000 Transfection Reagent

(14) Characterization of sclerostin's response within white adipose tissue to an obesogenic diet at rest and in response to acute exercise in male miceFrontiers in physiologyJanuary 1, 2022Kurgan N, Baranowski B, Stoikos J, MacNeil AJ, Fajardo VA, MacPherson REK, Klentrou P.analysis. Immunoblotting 20 µg of protein were loaded and resolved on 10% TGX fast cast gels (cat# 1610173, Bio-Rad, Hercules, California, United States) for 30 min at 250 V. Proteins were then semi-dry transferred

(15) Structure of a Ty1 restriction factor reveals the molecular basis of transposition copy number controlNature communicationsSeptember 22, 2021Cottee MA, Beckwith SL, Letham SC, Kim SJ, Young GR, Stoye JP, Garfinkel DJ, Taylor IA.was detected by running samples on a 10% TGX Stain-FreeTM FastCastTM Acrylamide gel (Bio-Rad cat. no. 1610173) and gel-imaging after 45 s of activation. Protein quantification with total protein normalization was

(16) Optimised tissue clearing minimises distortion and destruction during tissue delipidationNeuropathology and Applied NeurobiologyApril 1, 2021Krit Lee, Hei Ming Lai, Maja Hoejvang Soerensen, Edward Sai‐Kam Hui, Victor Wan‐San Ma, William Chi‐Shing Cho, Yuen‐Shan Ho, Raymond Chuen‐Chung Changprepared samples (48 μl per well) loaded into 10% stain‐free acrylamide gels (Mini‐PROTEAN® TGX, BioRad #1610173). The gel was run at 120 V for about 1.5 hours and visualised using stain‐free‐enabled imager (GelDoc Go

(17) A Salmonella Typhi RNA thermosensor regulates virulence factors and innate immune evasion in response to host temperaturePLoS pathogensMarch 1, 2021Brewer SM, Twittenhoff C, Kortmann J, Brubaker SW, Honeycutt J, Massis LM, Pham THM, Narberhaus F, Monack DM.Criterion 26-well (Bio-Rad #3459903) gels made with the 10% TGX FastCast acrylamide gel kit (Bio-Rad #1610173). The Precision Plus Protein Dual Color Standard (Bio-Rad #161–0394) was used for all gels, and they

(18) Resveratrol inhibits bile acid-induced gastric intestinal metaplasia via the PI3K/AKT/p-FoxO4 signalling pathwayPhytotherapy research : PTRMarch 1, 2021Lu W, Ni Z, Jiang S, Tong M, Zhang J, Zhao J, Feng C, Jia Q, Wang J, Yao T, et al.‐20°C freezer. Proteins were separated using a 7.5 to 12% TGX FastCast Acrylamide Kit (Lat No.1610171, 1610173, 1610175, Bio‐Rad Lab. Inc., USA). Then, the proteins were transferred onto a nitrocellulose membrane

(19) An Investigation of Fibulin-2 in Hypertrophic CardiomyopathyInternational journal of molecular sciencesSeptember 29, 2020Ibrahim AM, Roshdy M, Elshorbagy S, Hosny M, Halawa S, Yehia D, Elfawy HA, Eldessouki A, Mohamed F, Ellithy A, et al.denatured protein per sample were electrophoresed through 10% tris gel (Bio-rad, Hercules, CA, USA, #1610173) in the presence of 1xNuPage MOPS SDS running buffer (Invitrogen™, # NP0001-02). Proteins were transferred

(20) Pathological Conversion of Mouse Perivascular Adipose Tissue by Notch ActivationArteriosclerosis, Thrombosis, and Vascular BiologySeptember 1, 2020Joshua M. Boucher et al.before gel electrophoresis. SDS-PAGE was performed using Bio-Rad TGX FastCast gels between 10% and 12% (1610173 and 1610175) and 20 to 50 µg protein/lane. Gels were transferred to PVDF (polyvinylidene difluoride)

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