NEB Express Competent E. coli (High Efficiency) from New England Biolabs

Product Specs
ItemNEB Express Competent E. coli (High Efficiency)
CompanyNew England Biolabs
Price $225.00 Inquire
Catalog NumberC2523H
GenotypefhuA2 [lon] ompT gal sulA11 R(mcr-73::miniTn10--Tet^S)2 [dcm] R(zgb-210::Tn10--Tet^S) endA1 Δ(mcrC-mrr)114::IS10
Quantity20 x 0.05 ml
ApplicationsProteomics & Glycomics
Cell Type (standardized)Competent Cells
Validated Species ReactivityEscherichia
SpeciesE. coli
Temperature-80 C
Cell TypeCompetent Cells

Specifications/Features

Transformation Efficiency:
2 - 6 x 10⁸ cfu/µg

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducensNucleic Acids ResApril 24, 2026Benjamin F Jepson, Eric J Wolf, Anthony R Dawson, Jingmin Jin, Vladimir Potapov, Nan Dai, Ivan R Corrêa Jr., Erbay Yigit6×His affinity tag, and the gene of interest (Supplementary Fig. S2B). Competent E. coli cells (NEB, C2523) were transformed with the expression constructs and plated on LB agar supplemented with kanamycin (50

(2) Type II Restriction of 2-Aminoadenosine (dZ)-Modified DNA and Production of dZ-Modified Plasmid in E. coliVirusesFebruary 4, 2026Weiwei Yang, Michael S. Kuska, Nan Dai, Laurence M. Ettwiller, Ivan R. Corrêa, Shuang-Yong Xu(Mrr−, McrBC−, K strain), T7 shuffle (C3026, K strain), Nico (λDE3) (C2529, B strain), NEB Express (C2523, B strain). T7 Express (C2566) was used as a host for T7 expression vectors. pACYC-T7 was a derivative

(3) Nuclear pore passage of the HIV capsid is driven by its unusual surface amino acid compositionNature Structural & Molecular BiologyOctober 9, 2025Liran Fu, Shiya Cheng, Dietmar Riedel, Leonie Kopecny, Melina Schuh, Dirk Görlichconical CLPs His14–bdSUMO-tagged CA-P1A was expressed in NEB Express E. coli cells (New England Biolabs, C2523). Here, the P1A mutation is required for tag removal (that is, to allow SUMO cleavage). Induction was

(4) Application of post glycosylation modifying enzymes for mass spectrometry imaging of modified N -glycans in situBIORXIVMay 15, 2025Julia E. Dreifus, Léa Chuzel, Edwin E. Escobar, Grace Grimsley, Hongxia Bai, Andrew J. Hanneman, Samantha L. Fossa, Vanessa Gregory, Richard J. Martin, Richard R. Drake, et al.described16. The resulting plasmids were used to transform NEBExpress® Competent E. coli cells (NEB, USA C2523). Cultures were inoculated in 1 L of LB containing 50 μg/mL kanamycin and grown at 37°C with shaking

(5) The sigma factor σ54 (rpoN) functions as a global regulator of antibiotic resistance, motility, metabolism, and virulence in Clostridioides difficileFrontiers in MicrobiologyApril 29, 2025Ying Yang, Tingyu Huang, Junyi Yang, Ruirui Shao, Luhong Shu, Ping Ling, Yingjun Lu, Weihao Ma, Jian Liao, Zhizhong Guan, et al.used in this study are shown in Supplementary Table S1. NEBExpress Competent Escherichia coli cells (C2523H, New England BioLabs) were used for gene cloning and plasmid construction. The CA434 was used as the

(6) Proxi-RIMS-seq2 applied to native microbiomes uncovers hundreds of known and novel m5C methyltransferase specificitiesNucleic Acids ResMarch 29, 2025Weiwei Yang, Yvette Luyten, Emily Reister, Hayley Mangelson, Zach Sisson, Benjamin Auch, Ivan Liachko, Richard J Roberts, Laurence EttwillerTwo microliters of NEBuilder HiFi reaction was transformed into NEB Express competent E. coli cells (#C2523; gfhuA2 [lon] ompT gal sulA11 R (mcr-73::miniTn10–TetS)2 [dcm] R (zgb-210::Tn10–TetS) endA1 Δ (mcrC-mrr)114::IS10).

(7) Cellular and Molecular Effects of the Bruck Syndrome-Associated Mutation in the PLOD2 GeneInternational Journal of Molecular SciencesDecember 13, 2024Olga I. Bolshakova, Evgenia M. Latypova, Artem E. Komissarov, Alexandra D. Slobodina, Elena V. Ryabova, Elena Yu. Varfolomeeva, Olga E. Agranovich, Sergey F. Batkin, Svetlana V. Sarantsevathe ligation mixture was (5–10):1. Ligation was carried out overnight at 12 °C. The transformation of C2523 competent cells (New England BioLabs) by ligation mixtures was performed using the standard electroporation

(8) Proxi-RIMS-seq2 applied to native microbiomes uncovers hundreds of known and novel m5C methyltransferase specificitiesbioRxivJuly 21, 2024Weiwei Yang, Yvette Luyten, Emily Reister, Hayley Mangelson, Zach Sisson, Benjamin Auch, Ivan Liachko, Richard J. Roberts, Laurence Ettwillerstrain lacking endogenous m5C methylation while conserving m6A methylation at GATC sites (NEB Express C2523 strain, dcm-; dam+). The Oligoflexus methyltransferase was successfully cloned and genomic DNA from

(9) Protocol to detect OLA1 polyubiquitination by Aurora A in vivo and in vitroSTAR ProtocolsJune 21, 2024Zhenzhou Fang, Xingming Li, Yuki Yoshino, Natsuko Chiba(12–16 h) at 37°C.Alternatives: In this step, NEB express competent cells (New England Biolabs, Cat #C2523) can be used instead of BL21 competent cells.g.Pick three single, well-isolated colonies in 2 mL of

(10) Optimization of electroporation method and promoter evaluation for type-1 methanotroph, Methylotuvimicrobium alcaliphilumFrontiers in Bioengineering and BiotechnologyMay 15, 2024Shubhasish Goswami, Steven W. Singer, Blake A. Simmons, Deepika AwasthipurB20 φ80dlacZΔM15 Δ(lacZYA-argF) U169, hsdR17(r K – m K +), λ–",Invitrogen E. coli NEB Express® (#C2523),fhuA2 [lon] ompT gal sulA11 R(mcr-73::miniTn10—Tet S)2 [dcm] R(zgb-210::Tn10—Tet S) endA1 Δ(mcrC-mrr)114::IS10,NEB E. coli

(11) A checkpoint function for Nup98 in nuclear pore formation suggested by novel inhibitory nanobodiesThe EMBO JournalApril 22, 2024Mireia Solà Colom, Zhenglin Fu, Philip Gunkel, Thomas Güttler, Sergei Trakhanov, Vasundara Srinivasan, Kathrin Gregor, Tino Pleiner, Dirk Görlichproteins in E. coli Recombinant Nups were expressed in NEB Express E. coli cells (New England Biolabs, C2523). Nanobodies were expressed in E. coli SHuffle cells (New England Biolabs, C3026J) for disulfide bond

(12) An exonuclease-resistant chain-terminating nucleotide analogue targeting the SARS-CoV-2 replicase complexNucleic Acids ResearchFebruary 9, 2024Ashleigh Shannon, Aurélie Chazot, Mikael Feracci, Camille Falcou, Véronique Fattorini, Barbara Selisko, Steven Good, Adel Moussa, Jean-Pierre Sommadossi, François Ferron, et al.acid of the coding sequence of the protein. Nsp7 and nsp8 proteins were expressed in Escherichia coli C2523 cells (New England Biolabs, NEB Express) in the presence of the pRARE2LacI (Novagen) plasmid. Expression

(13) Demonstrating the importance of porcine reproductive and respiratory syndrome virus papain-like protease 2 deubiquitinating activity in viral replication by structure-guided mutagenesisPLoS pathogensDecember 1, 2023Bailey-Elkin BA, Knaap RCM, De Silva A, Boekhoud IM, Mous S, van Vught N, Khajehpour M, van den Born E, Kikkert M, Mark BL.in the same buffer. To purify the PRRSV DV PLP2 domain, pASK-DV399-578 was transformed into E. coli C2523 (NEB) cells and induced under the same conditions described for the SD01-08 PLP2 domain. Cell pellets

(14) Atypical Protein Kinase C Promotes its own Asymmetric Localisation by Phosphorylating Cdc42 in Polarising CellsbioRxivNovember 2, 2023John Packer, Alicia G. Gubieda, Aaron Brooks, Lars N. Deutz, Iolo Squires, Shona Ellison, Sundar Ram Naganathan, Adam J.M. Wollman, Daniel J. Dickinson, Josana RodriguezS71A: cacgtcggtctgtggataggctagaggccttaatcgatc. For protein production NEB Express Competent E coli (#C2523H) were transformed with the resulting plasmids. An overnight culture was used to inoculate 500 ml of

(15) Validation and the Determination of Antibody Bioactivity Using MILKSHAKE and Sundae ProtocolsMethods in Molecular BiologyOctober 1, 2023Mary R. Ferguson, Qiana M. Mendez, Felicity E. Acca, Cassandra D. Chapados, Holland A. Driscoll, Kezzia S. Jones, Gregory Mirando, Michael P. Weiner, Xiaofeng LiC-terminal sortase site. The protein is expressed in NEB Express Cells (New England BioLabs Catalog # C2523H). Genotype: fhuA2 [lon] ompT gal sulA11 R(mcr-73::miniTn10--TetS)2 [dcm] R(zgb-210::Tn10--TetS) end

(16) A dual mechanism of action of AT-527 against SARS-CoV-2 polymeraseNature CommunicationsDecember 1, 2022Ashleigh Shannon, VÃ©ronique Fattorini, Bhawna Sama, Barbara Selisko, Mikael Feracci, Camille Falcou, Pierre Gauffre, Priscila El Kazzi, Adrien Delpal, Etienne Decroly, et al.expressed under the control of a T5-promoter in pQE30 vectors in Escherichia coli (E. coli) NEB Express C2523 cells (New England Biolabs) carrying the pRare2LacI (Novagen) plasmid. Protein was expressed overnight

(17) A second type of N7-guanine RNA cap methyltransferase in an unusual locus of a large RNA virus genomeNucleic Acids ResearchOctober 28, 2022Ashleigh Shannon, Bhawna Sama, Pierre Gauffre, ThÃ©o Guez, FranÃ§oise Debart, Jean-Jacques Vasseur, Etienne Decroly, Bruno Canard, FranÃ§ois FerronHigh Fidelity Phusion Polymerase (NEB). Protein expression and purification Protein was expressed in C2523 (pLacI) E. coli overnight at 18°C from cultures induced with 0.4mM isopropyl-β-d-thiogalactopyranoside

(18) Favipiravir strikes the SARS-CoV-2 at its Achilles heel, the RNA polymerasebioRxivOctober 2, 2022A. Shannon, B. Selisko, NTT Le, J. Huchting, F. Touret, G. Piorkowski, V. Fattorini, F. Ferron, E. Decroly, C Meier, et al.inserting a GSGSGS linker between nsp7- and nsp8-coding sequences. Cofactors were expressed in NEB Express C2523 (New England Biolabs) cells carrying the pRare2LacI (Novagen) plasmid in the presence of Ampicillin

(19) Functional expression of diverse post-translational peptide-modifying enzymes in Escherichia coli under uniform expression and purification conditionsPLOS ONESeptember 19, 2022Emerson Glassey, Andrew M. King, Daniel A. Anderson, Zhengan Zhang, Christopher A. Voigtused to characterize RSTs and linker variants in low-throughput (flask) cultures. E. coli NEB Express (C2523I, New England BioLabs, Ipswich, MA, USA) was used to express all other experiments. All plasmids containing

(20) Development of HEK-293 Cell Lines Constitutively Expressing Flaviviral Antigens for Use in DiagnosticsMicrobiology SpectrumJune 29, 2022Jordan A. Powers, Benjamin Skinner, Brent S. Davis, Brad J. Biggerstaff, Lucy Robb, Elizabeth Gordon, William M. de Souza, Marcilio Jorge Fumagalli, Amanda E. Calvert, Gwong-Jen Changcontaining the gene cassettes were produced in Escherichia coli (NEB Express high-efficiency E. coli number C2523H; NEB, MA, USA) using manufacturer’s growth conditions, plasmid DNA extraction, and sequence confirmation

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