WesternBright Sirius- femotogram HRP Substrate from Advansta

Product Specs
ItemWesternBright Sirius- femotogram HRP Substrate
CompanyAdvansta
Price Pricing Info Get Pricing
Catalog NumberK-12043-D10
Quantity100ml
ApplicationsWestern blotting
Brand/TrademarkAdvansta

Specifications/Features

WesternBright® Sirius® is Advansta's most sensitive chemiluminescent substrate. Most sensitive of Advansta's HRP substrates, for low-abundance proteins. Long-lasting signal for flexible imaging. Low background for high signal-to-noise. Image by CCD or film. Includes: WesternBright® Sirius® Luminol/enhancer solution and WesternBright® Peroxide Chemiluminescent Detection Reagent

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Advansta

2140 Bering Drive

San Jose, California 95131-2013

United States

Phone: 650.325.1980
650.325.1904

Company Profile

Website: http://advansta.com/

(1) Thrombocyte-derived Dickkopf1 promotes macrophage polarization in the Bleomycin-induced lung injury modelFront ImmunolDecember 15, 2023Eun-Ah Sung, Min Hee Park, SuJeong Song, Hasan Alanya, Octavian Henegariu, Jinze Liu, E Zeynep Erson-Omay, Patricia J. Sime, Wook-Jin ChaeHRP-conjugated secondary antibodies, the membrane was developed using a WesternBright™ Sirius kit (K-12043-D10, Advansta). Bands were detected and quantified using the Chemidoc and ImageJ and normalized to β-actin

(2) Large-Scale Production of Anti-RNase A VHH Expressed in pyrG Auxotrophic Aspergillus oryzaeCurrent Issues in Molecular BiologyMay 31, 2023Elif Karaman, Alp Ertunga Eyüpoğlu, Lena Mahmoudi Azar, Serdar Uysalfrom GE Healthcare (Chicago, IL, USA) and a chemiluminescence detection kit (Western BrightTM Sirius, K-12043-D10) was purchased from Advansta (San Jose, CA, USA). Gangnam-Stain Protein Ladder (24052) was purchased

(3) CCP5 and CCP6 retain CP110 and negatively regulate ciliogenesisBMC biologyMay 24, 2023Wang Y, Zhang Y, Guo X, Zheng Y, Zhang X, Feng S, Wu HY.room temperature. The immunoreactivity of proteins was visualized with Western Bright ECL (Advansta, K-12043-D10, California, USA) using a Western Blot Imager (Vilber, Paris, France). Immunoprecipitation assay HEK293T

(4) The exocyst complex is an essential component of the mammalian constitutive secretory pathwayJournal of Cell BiologyMay 1, 2023Conceição Pereira, Danièle Stalder, Georgina S.F. Anderson, Amber S. Shun-Shion, Jack Houghton, Robin Antrobus, Michael A. Chapman, Daniel J. Fazakerley, David C. Gershlickfinally, their immunoreactivity was visualized using Clarity (1705061; Bio-Rad) or WesternBright Sirius (K-12043-D10; Advansta) ECL substrate, in the ChemiDoc Imaging System (Bio-Rad). Bands were quantified using Image Lab software

(5) A mutation in the ZNF687 gene that is responsible for the severe form of Paget's disease of bone causes severely altered bone remodeling and promotes hepatocellular carcinoma onset in a knock-in mouse modelBone researchMarch 14, 2023Russo S, Scotto di Carlo F, Maurizi A, Fortunato G, Teti A, Licastro D, Settembre C, Mello T, Gianfrancesco F.1 h at RT. The bands were visualized using enhanced chemiluminescence detection reagents (Advansta #K-12043-D10) and autoradiographic films (Aurogene #AU1101). Equal loading was confirmed by using antibodies against

(6) RNA localization mechanisms transcend cell morphologyeLifeMarch 3, 2023Goering R, Arora A, Pockalny MC, Taliaferro JM.hr. Blots were washed again in PBST and visualized using WesternBright HRP Substrate Kit (Advansta, K-12043-D10) and Sapphire biomolecular imager (Azure Biosystems) set to collect chemiluminescent signal. Neuronal

(7) Altered cell function and increased replication of rhinoviruses and EV-D68 in airway epithelia of asthma patientsFrontiers in MicrobiologyMarch 1, 2023Manel Essaidi-Laziosi, Léna Royston, Bernadett Boda, Francisco Javier Pérez-Rodriguez, Isabelle Piuz, Nicolas Hulo, Laurent Kaiser, Sophie Clément, Song Huang, Samuel Constant, et al.were washed and viral receptors were detected using an enhanced chemiluminescence solution (ECL, ref. K-12043-D10 Western Bright Sirius Advansta) for 2 min. Immunoblot images were acquired using Fujifilm LAS 4000 luminescence

(8) Interstitial Macrophages Lead Early Stages of Bleomycin-Induced Lung Fibrosis and Induce Fibroblasts ActivationCellsJanuary 24, 2023Sofia Libório-Ramos, Catarina Barbosa-Matos, Raquel Fernandes, Caroline Borges-Pereira, Sandra CostaBiomolecular Imager (Azure Biosystems, Dublin, CA, USA) using Western Bright Sirius HRP Substrate Kit (K-12043-D10, Advansta, San Jose, CA, USA). The bands were quantified in ImageJ software, and quantifications were

(9) Intrathecal Injection of the Secretome from ALS Motor Neurons Regulated for miR-124 Expression Prevents Disease Outcomes in SOD1-G93A MiceBiomedicinesAugust 29, 2022Marta Barbosa, Marta Santos, Nídia de Sousa, Sara Duarte-Silva, Ana Rita Vaz, António J. Salgado, Dora Britesmild agitation. Immunoreactive bands were detected after the incubation with Western Bright™ Sirius (K-12043-D10, Advansta, Menlo Park, CA, USA) using an iBrightTM FL1500 Imaging System. Bands were quantified using

(10) Clathrin adaptor AP-1–mediated Golgi export of amyloid precursor protein is crucial for the production of neurotoxic amyloid fragmentsJournal of Biological ChemistryAugust 1, 2022Yunan C. Januário et al.visualized using either Clarity (catalog no.: 1705061; Bio-Rad) or WesternBright Sirius (catalog no.: K-12043-D10; Advansta) enhanced chemiluminescence solutions and a ChemiDoc Imaging System equipped with the ImageLab

(11) SHLD1 is dispensable for 53BP1-dependent V(D)J recombination but critical for productive class switch recombinationNature communicationsJune 28, 2022Vincendeau E, Wei W, Zhang X, Planchais C, Yu W, Lenden-Hasse H, Cokelaer T, Pipoli da Fonseca J, Mouquet H, Adams DJ, et al.detected with Clarity Western ECL Substrate (Biorad 170-5060) or WesternBright Sirius substrate (Advansta K12043-D10). Blots were developed and analyzed using Image Lab v6.0 (Biorad). For XRCC4 blots, the membranes were

(12) Oligodendroglia-derived extracellular vesicles activate autophagy via LC3B/BAG3 to protect against oxidative stress with an enhanced effect for HSPB8 enriched vesiclesCell communication and signaling : CCSMay 5, 2022Van den Broek B, Wuyts C, Sisto A, Pintelon I, Timmermans JP, Somers V, Timmerman V, Hellings N, Irobi J.(ThermoFisher Scientific, 32132) for cell lysate or the WesternBright™ Sirius detection kit (Advansta, K-12043-D10) for EV lysate. Band intensities were determined by quantifying the mean pixel gray values using ImageJ

(13) Radiation-Induced Bystander Effect Mediated by Exosomes Involves the Replication Stress in Recipient CellsInternational Journal of Molecular SciencesApril 10, 2022Mateusz Smolarz, Łukasz Skoczylas, Marta Gawin, Monika Krzyżowska, Monika Pietrowska, Piotr Widłakdetection of bands was performed with WesternBright Sirius HRP substrate (Advansta, San Jose, CA, USA, K-12043-D10) according to the manufacturer’s instructions. 4.4. Immunocytochemistry and Fluorescence Microscopy Cells

(14) BMP-4 Extraction from Extracellular Matrix and Analysis of Heparin-Binding PropertiesMolecular BiotechnologyFebruary 1, 2022Senem Aykul, Jordan Maust, Erik Martinez-Hackertwas used as secondary antibody. WesternBright Sirius HRP substrate was used for detection (Advansta, K-12043-D10). Western blots were visualized by exposing the membrane to autoradiography film. Purification BMP-4

(15) SMURF1 and SMURF2 in Progenitor Cells from Articular Cartilage and Meniscus during Late-Stage OsteoarthritisCartilageDecember 1, 2021Schminke B, Kauffmann P, Schubert A, Altherr M, Gelis T, Miosge N.washing steps. Visualization of the proteins was achieved by applying WesternBright Sirius HRP substrate (K-12043-D10; Advansta). Exposed X-ray films were scanned for digitalization and densitometry was performed for quantification

(16) Nuclear Syndecan-1 Regulates Epithelial-Mesenchymal Plasticity in Tumor CellsBiologyJune 11, 2021Kumar-Singh A, Parniewska MM, Giotopoulou N, Javadi J, Sun W, Szatmári T, Dobra K, Hjerpe A, Fuxe J.Scientific) for 1 h at room temperature. For chemiluminescent detection, chemiluminescent HRP Substrate (K-12043-D10; Advansta/AH Diagnostics, Solna, Sweden) was added and the membrane was incubated for 1 min. The Odyssey

(17) α-Tocopherol Attenuates Oxidative Phosphorylation of CD34⁺ Cells, Enhances Their G0 Phase Fraction and Promotes Hematopoietic Stem and Primitive Progenitor Cell MaintenanceBiomoleculesApril 10, 2021Rodriguez L, Duchez P, Touya N, Debeissat C, Guitart AV, Pasquet JM, Vlaski-Lafarge M, Brunet de la Grange P, Ivanovic Z.immunoreactive proteins were detected by using the WesternBright Sirius Chemiluminescent Detection Kit (#K-12043-D10, Advansta, San Jose, CA, USA) and a Fusion Solo S6 edge device (Vilber Lourmat, France). Quantum software

(18) Rosmarinic acid inhibits cell proliferation, migration, and invasion and induces apoptosis in human glioma cellsInternational Journal of Molecular MedicineFebruary 26, 2021Yunsheng Liu, Xiangping Xu, Han Tang, Yuchen Pan, Bing Hu, Guodong Huangadded for 1 h at room temperature. The membranes were visualized with Western Bright™ reagent (cat. no. K‑12043‑D10; Advansta, Inc.). The band signals were captured by The ChemiDoc MP system (Bio‑Rad Laboratories, Inc.).

(19) Pirfenidone Alleviates Choroidal Neovascular Fibrosis through TGF-β/Smad Signaling PathwayJournal of OphthalmologyFebruary 10, 2021Chuang Gao, Xin Cao, Lili Huang, Yueqi Bao, Tao Li, Yue Di, Liucheng Wu, Yu Songwere then incubated with the chemoﬂuorescent reagent enhanced chemiluminescence (Western Bright ECL, K-12043-D10, Advansta, USA) and exposed to X-ray ﬁlm in the dark. The intensity of the GAPDH signal was used as

(20) Microglial derived extracellular vesicles activate autophagy and mediate multi‐target signaling to maintain cellular homeostasisJournal of Extracellular VesiclesNovember 25, 2020Bram Van den Broek et al.(ThermoFisher Scientific, 32132) for cell lysate or the WesternBright Sirius detection kit (Advansta, K‐12043‐D10) for EVs lysate. Band intensities were determined by quantifying the mean pixel grey values using the

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4/8/2014Detection of Low Abundant Proteins Song ChenWe are interested in understanding the regulation of protein stability of those important low abundant proteins with short half-life in tumor cells, such as Hif1alpha, Nrf2, p53, p21 and p27. One of ... Read Review

WesternBright Sirius- femotogram HRP Substrate from Advansta

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Advansta

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WesternBright Sirius- femotogram HRP Substrate from Advansta

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