BioFlo® 320 Bioprocess / Bioreactor System from Eppendorf

Product Specs
ItemBioFlo® 320 Bioprocess / Bioreactor System
CompanyEppendorf
Price Get Pricing
Catalog NumberBioFlo 320
QuantityEA
Volume2.5 to 14 L (Autoclavable)
1.3 to 50 L (Single-use)
Capacity0.6 to 10.5 L (Autoclavable)
0.25 to 40 L (Single-use)
TypeAutoclavable and Single-use
ApplicationsCell culture and microbiology, Cultivation of mammalian, insect, human cell lines, Stem cell culture, Biofeul / biopolymer development, Vaccine production in anchorage / non-anchorage dependent cell lines, Laboratory and pilot scale fermentation of aerobic / anaerobic bacteria, yeasts and fungi
Dimensions(WxDxH) 16 x 16 x 26 in. (Control Station)
Electrical Requirements100 to 120/208 to 240 VAC, 50/60 Hz
Interface15 in. Projected Capacitive Touchscreen (Control Station)

Specifications/Features

Vessels Interchangeable: Yes
Sterilization: Autoclavable and Single-Use
PC-Compatible: Yes
Vessel Size: 2.5 to 14 L (Autoclavable), 1.3 to 50 L (Single-use)

Operating Modes:

Suitable for batch, fed batch, and continuous processes
Laboratory and pilot scale fermentation
Appropriate for specialized applications and cultivation of various cell-lines

working volume: 0.6 to 10.5 L (Autoclavable)
0.25 to 40 L (Single-use)

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Local Office

Eppendorf

100 Crossing Boulevard

Framingham, MA 01702

United States

Phone: 1-800-645-3050

Company Profile

Website: www.eppendorf.com

International Office

Eppendorf

Barkhausenweg 1

Hamburg 22339

Germany

Phone: +49 (0)1803 255 911

Email: eppendorf@eppendorf.de

Company Profile

Website: http://www.eppendorf.com/

(1) Hierarchical design of pseudosymmetric protein nanoparticlesResearch SquareJuly 11, 2023Quinton M. Dowling, Young-Jun Dowling, Young-Jun Gerstenmaier, Erin C. Gerstenmaier, Erin Wargacki, Adam Wargacki, Chelsea N. Fries, Chelsea Ravichandran, Rashmi Ravichandran, Anika Burrell, et al.expressed in Lemo21(DE3) cells (NEB) in LB (10 g Tryptone, 5 g Yeast Extract, 10 g NaCl) grown in a 10 L BioFlo 320 Fermenter (Eppendorf). At inoculation, impeller speed was set to 225 rpm, SPLM set to 5 with O2 supplementation

(2) Hierarchical design of pseudosymmetric protein nanoparticlesbioRxivJune 21, 2023Quinton M. Dowling, Young-Jun Park, Neil Gerstenmaier, Erin C. Yang, Adam Wargacki, Yang Hsia, Chelsea N. Fries, Rashmi Ravichandran, Carl Walkey, Anika Burrell, et al.expressed in Lemo21(DE3) cells (NEB) in LB (10 g Tryptone, 5 g Yeast Extract, 10 g NaCl) grown in a 10 L BioFlo 320 Fermenter (Eppendorf). At inoculation, impeller speed was set to 225 rpm, SPLM set to 5 with O2 supplementation

(3) Glutathione affinity chromatography for the scalable purification of an oncolytic virus immunotherapy from microcarrier cell cultureFrontiers in Bioengineering and BiotechnologyJune 15, 2023Andrew R. Swartz, Yvonne Shieh, Amanda Gulasarian, Erik Curtis, Carl F. Hofmann, Jack B. Baker, Neil Templeton, Jessica W. Olsonbioreactor cell culture At the laboratory scale, MRC-5 cells were cultured in 3 L glass bioreactors with a BioFlo 320 controller (Cat#: 1379962911, Eppendorf, Hamburg, Germany) on 1 g/L Cytodex 1 microcarriers (Cat#: 17548702,

(4) rAAV expressing recombinant antibody for emergency prevention and long-term prophylaxis of COVID-19Frontiers in ImmunologyMarch 30, 2023Ilias B. Esmagambetov, Ekaterina I. Ryabova, Artem A. Derkaev, Dmitry V. Shcheblyakov, Inna V. Dolzhikova, Irina A. Favorskaya, Daria M. Grousova, Mikhail A. Dovgiy, Vladimir V. Prokofiev, Andrey I. Gosudarev, et al.collection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a BioFlo 320 bioreactor (Eppendorf, Germany) with a BioBLU 5c disposable vessel (Eppendorf, Germany) filled with

(5) Development and scale-up of rVSV-SARS-CoV-2 vaccine process using single use bioreactorBiotechnology ReportsMarch 1, 2023Christopher Ton, Victoria Stabile, Elizabeth Carey, Adam Maraikar, Travis Whitmer, Samantha Marrone, Nelson Lee Afanador, Igor Zabrodin, Greeshma Manomohan, Melissa Whiteman, et al.for rVSV-SARS-CoV-2 production in 3 L bioreactors Process optimization was performed with Eppendorf BioFlo320 3 L glass bioreactors with pitched 3-blade impellers and a 3 L working volume. Cells from static cell

(6) Melanin biopolymer synthesis using a new melanogenic strain of Flavobacterium kingsejongi and a recombinant strain of Escherichia coli expressing 4-hydroxyphenylpyruvate dioxygenase from F. kingsejongiMicrobial Cell FactoriesDecember 1, 2022Han Sae Lee, Jun Young Choi, Soon Jae Kwon, Eun Seo Park, Byeong M. Oh, Jong H. Kim, Pyung Cheon Leeat 25 °C until the OD600 reached 2–3. The preculture was then transferred into a 5-L jar fermenter (BioFlo 320, Eppendorf, Framingham, MA, USA) containing 1.5 L modified TB medium supplemented with 10 g/L tyrosine.

(7) Intracerebroventricular dosing of N-sulfoglucosamine sulfohydrolase in mucopolysaccharidosis IIIA mice reduces markers of brain lysosomal dysfunctionJournal of Biological ChemistryDecember 1, 2022Jenna Magat, Samantha Jones, Brian Baridon, Vishal Agrawal, Hio Wong, Alexander Giaramita, Linley Mangini, Britta Handyside, Catherine Vitelli, Monica Parker, et al.Efficient Feed B (ThermoFisher) added during the run along with proprietary additives. The bioreactors (10L BioFlo 320, Eppendorf or 10L Applikon, Applikon Biotechnology) were monitored for viable cell density, dissolved

(8) Induction of cross-neutralizing antibodies by a permuted hepatitis C virus glycoprotein nanoparticle vaccine candidateNature communicationsNovember 25, 2022Sliepen K, Radić L, Capella-Pujol J, Watanabe Y, Zon I, Chumbe A, Lee WH, de Gast M, Koopsen J, Koekkoek S, et al.expression and purification Lemo21 (DE3) (NEB) cells expressing I53-50B.4PT1 were grown in a 10 L BioFlo 320 Fermenter (Eppendorf) or a 2 L shake flask. Cells were grown in LB (10 g Tryptone, 5 g Yeast Extract,

(9) Adaptation of a microbial community to demand-oriented biological methanationBiotechnol Biofuels BioprodNovember 16, 2022Hoda Khesali Aghtaei, Sebastian PÃ¼ttker, Irena Maus, Robert Heyer, Liren Huang, Alexander Sczyrba, Udo Reichl, Dirk Benndorfreactor The biogas reactor, a stainless-steel dish bottom vessel (volume 2.5 L) was controlled via a BioFlo®320 system (Eppendorf, Inc., Enfield, CT, USA). The active culture (2.1 L working volume) was stirred continuously

(10) The transcriptomic landscape of Magnetospirillum gryphiswaldense during magnetosome biomineralizationBMC GenomicsOctober 10, 2022Cornelius N. Riese, Manuel Wittchen, ValÃ©rie JÃ©rÃ´me, Ruth Freitag, Tobias Busche, JÃ¶rn Kalinowski, Dirk SchÃ¼lerused for RNA isolation and cDNA library preparation were cultivated in a stirred-tank 3 L bioreactor (BioFlo™ 320, Eppendorf Bioprocess, Jülich, Germany) equipped with an InPro3253i (Mettler-Toledo, Columbus, USA)

(11) rAAV expressing recombinant neutralizing antibody for the botulinum neurotoxin type A prophylaxisFrontiers in MicrobiologySeptember 27, 2022Artem A. Derkaev, Ekaterina I. Ryabova, Ilias B. Esmagambetov, Dmitry V. Shcheblyakov, Svetlana A. Godakova, Irina D. Vinogradova, Anatoly N. Noskov, Denis Y. Logunov, Boris S. Naroditsky, Alexander L. Gintsburgcollection of the Gamaleya National Research Center for Epidemiology and Microbiology) and cultured in a BioFlo 320 bioreactor (Eppendorf, Germany) with a BioBLU 5c disposable vessel (Eppendorf, Germany) filled with

(12) Vaccination with a structure-based stabilized version of malarial antigen Pfs48/45 elicits ultra-potent transmission-blocking antibody responsesImmunitySeptember 13, 2022Brandon McLeod, Moustafa T. Mabrouk, Kazutoyo Miura, Rashmi Ravichandran, Sally Kephart, Sophia Hailemariam, Thao P. Pham, Anthony Semesi, Iga Kucharska, Prasun Kundu, et al.(NEB) in LB (10 g Tryptone, 5 g Yeast Extract, 10 g NaCl) grown in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf). Cells were grown at 37°C to an OD600 ∼0.8, and then induced with 1 mM IPTG. Expression

(13) Distinct sensitivities to SARS-CoV-2 variants in vaccinated humans and miceCell ReportsAugust 30, 2022Alexandra C. Walls et al.(NEB) in LB (10 g Tryptone, 5 g Yeast Extract, 10 g NaCl) grown in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf). Cells were grown at 37°C to an OD600 ∼0.8, and then induced with 1 mM IPTG. Expression

(14) An integrated and continuous downstream process for microbial virusâ€like particle vaccine biomanufactureBiotechnology and BioengineeringAugust 1, 2022Lukas Gerstweiler, Jagan Billakanti, Jingxiu Bi, Anton Peter Jacob Middelberg2021c). The pH value in the vessel was controlled at a value of pH 7.2. This was implemented with a BioFlo® 320 control panel (Eppendorf, Germany) with 1% v v−1 HCl, 1 M NaCl, 5% w w−1 glycerol as an acid solution

(15) Elicitation of broadly protective sarbecovirus immunity by receptor-binding domain nanoparticle vaccinesbioRxivJuly 22, 2022Alexandra C. Walls et al.(NEB) in LB (10 g Tryptone, 5 g Yeast Extract, 10 g NaCl) grown in 2 L baffled shake flasks or a 10 L BioFlo 320 Fermenter (Eppendorf). Cells were grown at 37°C to an OD600 ~0.8, and then induced with 1 mM IPTG. Expression

(16) Non-Native Amino Acid Click Chemistry-Based Technology for Site-Specific Polysaccharide Conjugation to a Bacterial Protein Serving as Both Carrier and Vaccine AntigenACS OmegaJuly 19, 2022Neeraj Kapoor et al.express the GlcNAc side chain), was grown for 20–24 h and then utilized to inoculate a 10 L Eppendorf BioFlo 320 bioreactor containing growth medium with primary components being lactose (Spectrum, Cat # LA103, 300

(17) Wild Heterotrophic Nitrifying Strain Pseudomonas BT1 Isolated from Kitchen Waste Sludge Restores Ammonia Nitrogen Removal in a Sewage Treatment Plant Shocked by ThioureaApplied Biochemistry and BiotechnologyJuly 1, 2022Jingxuan Deng, Zhenxing Huang, Jinbo Wang, Xiaohong Shan, Wansheng Shi, Wenquan RuanThe reactors were made of polymethyl methacrylate (PMMA) and were equipped with a control station (BioFlo 320, Eppendorf, Germany), which was connected with a pH and DO meter (PO720, Mettler Toledo, Switzerland),

(18) Two-Component Spike Nanoparticle Vaccine Protects Macaques from SARS-CoV-2 InfectionSSRN Electronic JournalMarch 30, 2022Philip J. M. Brouwer et al.(ProtParam). I53-50B.4PT1 expression and purification Lemo21 (DE3) (NEB) cells expressing I53-50B.4PT1 were grown in a 10L BioFlo 320 Fermenter (Eppendorf) or a 2L shake flask. Cells were grown in LB (10 g Tryptone, 5 g Yeast Extract,

(19) Organelle Engineering in Yeast: Enhanced Production of Protopanaxadiol through Manipulation of Peroxisome Proliferation in Saccharomyces cerevisiaeMicroorganismsMarch 18, 2022Bo Hyun Choi, Hyun Joon Kang, Sun Chang Kim, Pyung Cheon LeeFermentation Batch fermentation was performed at 30 °C, pH 5.5, and a dissolved oxygen (DO) level >50% in a 3.5 L BioFlo 320 bioreactor (Eppendorf, Hamburg, Germany) containing 1.5 L of the SD, YPD, or YPDO medium. The DO level

(20) Nutrient-depended metabolic switching during batch cultivation of Streptomyces coelicolor explored with absolute quantitative mass spectrometry-based metabolite profiling3 BiotechMarch 1, 2022Kanhaiya Kumar, Per Bruheimal. 2012a; Kumar and Bruheim 2021b). The cultivations were conducted in 3 L bioreactors (Eppendorf Bioflo 320) with an initial working volume of 1.8 L and the inoculum was pre-germinated spores having ~ 1 × 109

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