BioFlo® 120 Bioprocess Control Station from Eppendorf

(1) Expansion of bovine skeletal muscle stem cells from spinner flasks to benchtop stirred-tank bioreactors for up to 38 daysFront NutrAugust 7, 2023Dimitrios Tzimorotas, Nina Therese Solberg, R. Christel Andreassen, Panagiota Moutsatsou, Vincent Bodiou, Mona Elisabeth Pedersen, Sissel Beate Rønningindependent biological replicate experiments were performed in the benchtop stirred-tank bioreactor (BioFlo® 120 benchtop stirred-tank bioreactor, Eppendorf, AG, Hamburg, Germany), using MuSCs isolated from different

(2) Efficient production of bacterial antibiotics aminoriboflavin and roseoflavin in eukaryotic microorganisms, yeastsMicrobial Cell FactoriesJuly 20, 2023Kostyantyn V. Dmytruk, Justyna Ruchala, Liubov R. Fayura, Grzegorz Chrzanowski, Olena V. Dmytruk, Andriy O. Tsyrulnyk, Yuliia A. Andreieva, Daria V. Fedorovych, Olena I. Motyka, Diethard Mattanovich, et al.Fermentations were carried out in 1 L working volume bioreactors with a computer-based process control (BioFlo 120, Eppendorf, Germany). Fermentation temperature was controlled at 25 °C, pH at 5.0, with addition of

(3) Metabolic engineering of Corynebacterium glutamicum for fatty alcohol production from glucose and wheat straw hydrolysateBiotechnology for Biofuels and BioproductsJuly 18, 2023Felix Werner, Lynn S. Schwardmann, Daniel Siebert, Christian Rückert-Reed, Jörn Kalinowski, Marie-Theres Wirth, Katharina Hofer, Ralf Takors, Volker F. Wendisch, Bastian Blombachheat shock at 46 °C [75, 76]. Bioreactor cultivation Fed-batch cultivations were conducted in a 1 L BioFlo120® bioreactor system (Eppendorf SE, Hamburg, Germany) with an initial volume of 0.5 L. The cultivations

(4) Evidence of the Dysbiotic Effect of Psychotropics on Gut Microbiota and Capacity of Probiotics to Alleviate Related Dysbiosis in a Model of the Human ColonInternational Journal of Molecular SciencesApril 15, 2023Yasmina Ait Chait, Walid Mottawea, Thomas A. Tompkins, Riadh Hammamifermentation procedure was initiated by transferring 60 mL of immobilized gel beads into the IR (1L BioFlo® 120 vessel; Eppendorf, Mississauga, ON, Canada) containing 140 mL fresh sterile Macfarlane culture medium

(5) Bi-terminal fusion of intrinsically-disordered mussel foot protein fragments boosts mechanical strength for protein fibersNature CommunicationsApril 14, 2023Jingyao Li, Bojing Jiang, Xinyuan Chang, Han Yu, Yichao Han, Fuzhong Zhangacetate hydrate, 14.1 mg/L EDTA, 50 mg/L kanamycin, 0.01% v/v antifoam 204), and transferred to a 2 L Bioflo120 fed-batch bioreactor (Eppendorf, Hamburg, Germany). The culturing was controlled at 29 °C, and the pH

(6) Novel evolved Yarrowia lipolytica strains for enhanced growth and lipid content under high concentrations of crude glycerolMicrobial Cell FactoriesMarch 31, 2023Asimina Tsirigka, Eleni Theodosiou, Sotiris I. Patsios, Antiopi Tsoureki, Aggeliki Andreadelli, Elisavet Papa, Amalia Aggeli, Anastasios J. Karabelas, Antonios M. Makrischaracterization (25 mL, 150 rpm, 30 °C) using synthetic nitrogen-limited media. A 3-L bench-top bioreactor (BioFlo120, Eppendorf, Germany) was also used to run batch cultivations, to evaluate the performance of the selected

(7) Biofabrication of Functional Pullulan by Aureobasidium pullulans under the Effect of Varying Mineral Salts and Sugar Stress ConditionsMoleculesMarch 8, 2023Katia Van den Eynde, Vik Boon, Rita Caiado Gaspar, Pedro FardimpH, temperature, agitation speed and aeration rate in the vessel were controlled automatically by the BioFlo 120 bioprocess controller from Eppendorf. Precise temperature control was provided through a heat blanket

(8) Thermophilic Filamentous Fungus C1-Cell-Cloned SARS-CoV-2-Spike-RBD-Subunit-Vaccine Adjuvanted with Aldydrogel®85 Protects K18-hACE2 Mice against Lethal Virus ChallengeVaccines (Basel)December 11, 2022Ram Nechooshtan, Sharon Ehrlich, Marika Vitikainen, Arik Makovitzki, Eyal Dor, Hadar Marcus, Idan Hefetz, Shani Pitel, Marilyn Wiebe, Anne Huuskonen, et al.These flask cultures were incubated at 37 °C, 250 rpm for 24 h. RBD-C-tag was manufactured in a 10 L Bioflo 120 Fermenter (Eppendorf, Hamburg, Germany), and 5 L of the high concentration yeast extract medium (glucose,

(9) Improved Itaconate Production with Ustilago cynodontis via Co-Metabolism of CO2-Derived FormateJournal of FungiDecember 5, 2022Lena Ullmann, Nils Guntermann, Philipp Kohl, Gereon Schröders, Andreas Müsgens, Giancarlo Franciò, Walter Leitner, Lars M. Blankwhen a stable itaconate production was observed. Controlled-fed-batch cultivations were performed in a BioFlo® 120 bioreactor with a total volume of 1.3 L and a working volume of 0.5 L in combination with DASware Control

(10) Epicardial slices: an innovative 3D organotypic model to study epicardial cell physiology and activationnpj Regenerative MedicineDecember 1, 2022D. Maselli, R. S. Matos, R. D. Johnson, C. Chiappini, P. Camelliti, P. Campagnoloprinted adapter was inserted between the Petri dish and its lid providing inlet/outlet connection to the BioFlo 120 (Eppendorf) control station which provided a real-time feedback regulation of the medium pH at 7.4,

(11) Fungal dual-domain LysM effectors undergo chitin-induced intermolecular, and not intramolecular, dimerizationPlant PhysiologyOctober 27, 2022Hui Tian, Gabriel L Fiorin, Anja Kombrink, Jeroen R Mesters, Bart P H J ThommaScientific, California, USA). Fermentation was conducted in approximately 3 L of culture in a bioreactor BioFlo120 (Eppendorf, Hamburg, Germany) at 30°C for 5 d, including 3 d of methanol induction. Next, P. pastoris

(12) Survival and Interplay of γ-Aminobutyric Acid-Producing Psychobiotic Candidates with the Gut Microbiota in a Continuous Model of the Human ColonBiology (Basel)September 4, 2022Rojaalsadat Mousavi, Walid Mottawea, Marie-Claude Audet, Riadh Hammamidescribed [27]. The model consisted of a two-stage design comprising of an inoculation reactor (IR) (1L BioFlo® 120 vessel; Eppendorf, Mississauga, ON, Canada), with immobilized fecal microbiota used to continuously

(13) The Effect of Heat Sterilization on Key Filtration Performance Parameters of a Commercial Polymeric (PVDF) Hollow-Fiber Ultrafiltration MembraneMembranes (Basel)July 22, 2022Alexandra Nastouli, Asimina Tsirigka, Michael Harasek, Anastasios J. Karabelas, Sotiris I. Patsiosmodules were designed to be suitable for the head-plate and the height of a 3 L lab-scale bioreactor (BioFlo 120, Eppendorf, Hamburg, Germany). The main characteristics of the membranes used are summarized in Table

(14) Cytotoxic activity of Nep1‐like proteins on monocotsNew PhytologistJuly 1, 2022Maikel B. F. Steentjes et al.Pichia pastoris strain GS115 by electroporation. Fermentation was performed on a large scale using a BioFlo 120 fermentor (Eppendorf, Hamburg, Germany), applying the fermentation protocol described in Schouten et

(15) Development of an E. coli-based norbaeocystin production platform and evaluation of behavioral effects in ratsMetabolic Engineering CommunicationsJune 1, 2022Alexandra M. Adams, Nicholas A. Anas, Abhishek K. Sen, Jordan D. Hinegardner-Hendricks, Philip J. O’Dell, William J. Gibbons, Jessica E. Flower, Matthew S. McMurray, J. Andrew JonesHPLC and LC-MS analysis. 4.4 Scale-up studies All bioreactor studies were performed in an Eppendorf BioFlo120 bioreactor with 1.5 L working volume. The vessel was mixed using a direct drive shaft containing two

(16) Toxicity and Local Tolerance of a Novel Spike Protein RBD Vaccine Against SARS-CoV-2, Produced Using the C1 Thermothelomyces Heterothallica Protein Expression PlatformToxicologic PathologyMay 6, 2022Yuval Ramot et al.These flask cultures were incubated at 37°C, 250 rpm for 24 hours. C1-RBD was manufactured in a 10-L Bioflo 120 Fermenter (Eppendorf, Hamburg). Five liters of high concentration yeast extract medium with glucose

(17) A fermentation process for the production of poly(3-hydroxybutyrate) using waste cooking oil or waste fish oil as inexpensive carbon substrateBiotechnology ReportsMarch 1, 2022Tran Thi Loan, Dao Thi Quynh Trang, Pham Quang Huy, Pham Xuan Ninh, Doan Van Thuocinoculate 1.35 L of MP1 containing 2% (w/v) WCO or WFO as carbon source in a 3 L bioreactor (Eppendorf BioFlo 120). Batch fermentation mode was used out at 30 °C, antifoam was added when needed, and the pH of the culture

(18) BdGUCD1 and Cyclic GMP Are Required for Responses of Brachypodium distachyon to Fusarium pseudograminearum in the Mechanism Involving JasmonateInternational Journal of Molecular SciencesFebruary 28, 2022Maria Duszyn, Brygida Świeżawska-Boniecka, Monika Skorupa, Krzysztof Jaworski, Adriana Szmidt-Jaworskafinal concentration of 1 mM and incubating the cells at 22 °C for 3 h in glass vessels connected to a BioFlo 120 bioprocess control station (Eppendorf, Hauppauge, NY, USA). The pH was maintained at 6.5 (±0.2), the dissolved oxygen parameter

(19) Porcine Organotypic Epicardial Slice Protocol: A Tool for the Study of Epicardium in Cardiovascular ResearchFrontiers in cardiovascular medicineJanuary 1, 2022Maselli D, Matos RS, Johnson RD, Martella D, Caprettini V, Chiappini C, Camelliti P, Campagnolo P.File 2)8 mm-high Polydimethylsiloxane (PDMS) pillars arrayAnatomical forceps, curved, 10cm (Graefe)BioFlo120® control station (Eppendorf)CO2 incubator (Galaxy® 48R – Eppendorf)Entomologist pins (A1 - 0.14 × 10

(20) Engineering acetyl-CoA supply and ERG9 repression to enhance mevalonate production in Saccharomyces cerevisiaeJournal of Industrial Microbiology and BiotechnologyDecember 23, 2021Scott A Wegner, Jhong-Min Chen, Samantha S Ip, Yanfei Zhang, Deepak Dugar, José L Avalosgrown overnight. The next day, the overnight culture was used to inoculate the 2 L fermentor (Eppendorf BioFlo® 120, Hamburg, Germany) containing 1 L fermentation media (SC-dropout media containing 20 g/l glucose, 15

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BioFlo® 120 Bioprocess Control Station from Eppendorf

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BioFlo® 120 Bioprocess Control Station from Eppendorf

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