Q5 Hot Start High-Fidelity 2X Master Mix M0494S from New England Biolabs

Product Specs
ItemQ5 Hot Start High-Fidelity 2X Master Mix
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0494S
Quantity100 reactions
ApplicationsDNA Analysis
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeMaster Mix
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) PRR14 organizes H3K9me3-modified heterochromatin at the nuclear laminaNucleus (Austin, Tex.)December 1, 2023Kiseleva AA, Cheng YC, Smith CL, Katz RA, Poleshko A.pCMV6-AN-mGFP-PRR14 full-length vectors using Q5 Hot-start High-Fidelity Polymerase 2x Mix (New England Biolabs, cat# M0494S) and the following primers containing HindIII and RsrII restriction sites (Forward (HindIII): 5’- GCTTCTCAAGCTTGTACCATCCATGGACTTGCCCGG-3’;

(2) Differentiated cultures of an immortalized human neural progenitor cell line do not replicate prions despite PrP(C) overexpressionPrionDecember 1, 2023Slota JA, Wang X, Lusansky D, Medina SJ, Booth SA.50 ng of genomic DNA were amplified with the Q5 Hot Start High-Fidelity 2× PCR Master Mix (cat no. M0494L, NEB) using primers 5’-TCTTTGTGACTATGTGGACTG-3’ and 5’-TGCCACATGCTTGAGGTTGGTT-3’. The PCR products were

(3) Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populationsSTAR ProtocAugust 15, 2023Miriam Merenciano, Laura Aguilera, Josefa GonzálezPropionic acid,Merck,Cat # 81910-1L Nipagin,Merck,Cat # H5501-500GR Q5 Hot Start High-Fidelity 2× Master Mix,NEB,Cat # M0494S DreamTaq Green PCR Master Mix (2×),Thermo Fisher,Cat # K1081 ,, Critical commercial assays,Critical

(4) Targeting Sex Determination to Suppress Mosquito PopulationsResearch SquareJuly 27, 2023Omar Akbari, Ming Li, Nikolay Kandul, Ruichen Sun, Ting Yang, Elena Dalla Dalla Benetta, Daniel Brogan, igor antoshechkin, Héctor Sánchez Sánchez C, Yin Peng Zhan, et al.(Integrated DNA Technologies) using Q5 Hotstart Start High-Fidelity 2X Master Mix (New England Biolabs, Cat. #M0494S). The generated plasmids were transformed into Zymo JM109 chemically competent E. coli (Zymo Research,

(5) The contributions of DNA accessibility and transcription factor occupancy to enhancer activity during cellular differentiationbioRxivJuly 27, 2023Trevor Long, Tapas Bhattacharyya, Andrea Repele, Madison Naylor, Sunil Nooti, Shawn Krueger, Manuand the longer amplicon. The in/out PCRs were carried out with Q5 polymerase (New England BioLabs, M0494S), while the out/out PCRs were carried out with PrimeSTAR GXL DNA Polymerase (Takara Bio, R051A). Seamless

(6) Regulation of store-operated Ca2+ entry by IP3 receptors independent of their ability to release Ca2+eLifeJuly 19, 2023Pragnya Chakraborty, Bipan Kumar Deb, Vikas Arige, Thasneem Musthafa, Sundeep Malik, David I Yule, Colin W Taylor, Gaiti Hasanmodifications were carried out using Q5 Hot Start high-fidelity 2 X Master Mix (New England BioLabs, Cat# M0494L) using the recommendations of the manufacturer. Primers used in this study (details given in Supplementary

(7) SPIDR: a highly multiplexed method for mapping RNA-protein interactions uncovers a potential mechanism for selective translational suppression upon cellular stressbioRxivJuly 17, 2023Erica Wolin, Jimmy K. Guo, Mario R. Blanco, Andrew A. Perez, Isabel N. Goronzy, Ahmed A. Abdou, Darvesh Gorhe, Mitchell Guttman, Marko Jovanovicseparate tube. Biotinylated oligo tags were amplified on-bead using 2X Q5 Hot-Start Mastermix (NEB #M0494) with primers that add the indexed full Illumina adaptor sequences. To isolate barcoded cDNA, the supernatant

(8) Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathyNature CommunicationsJune 22, 2023Markus Grosch, Laura Schraft, Adrian Chan, Leonie Küchenhoff, Kleopatra Rapti, Anne-Maud Ferreira, Julia Kornienko, Shengdi Li, Michael H. Radke, Chiara Krämer, et al.Nextera-compatible adapters (Supplementary Data 6) using Q5® Hot Start High-Fidelity 2× Master Mix (M0494L, NEB). One microliter of a 1:100 dilution was used for a second PCR attaching sample-specific index

(9) Engineered autocrine signaling eliminates muscle cell FGF2 requirements for cultured meat productionbioRxivJune 19, 2023Andrew J. Stout, Xiaoli Zhang, Sophia M. Letcher, Miriam L. Rittenberg, Michelle Shub, Kristin M. Chai, Maya Kaul, David L. Kaplanperformed via gibson assemblies. This involved amplification with Q5 high-fidelity DNA polymerase (NEB #M0494S, Ipswich, MA, USA), DpnI digestion (NEB #R0176S), PCR cleanup (NEB #T1030S), assembly with the NEBuilder

(10) Calling Cards: a customizable platform to longitudinally record protein-DNA interactions over time in cells and tissuesbioRxivJune 15, 2023Allen Yen, Chase Mateusiak, Simona Sarafinovska, Mariam A. Gachechiladze, Juanru Guo, Xuhua Chen, Arnav Moudgil, Alexander J. Cammack, Jessica Hoisington-Lopez, MariaLynn Crosby, et al.distribution of barcodes that were packaged. Materials: Q5 Hot Start High-Fidelity Master Mix (NEB M0494) AMPure XP Reagent (Beckman Coulter A63882) or Mag-Bind TotalPure NGS beads (Omega Biotek M1378-02) NOTE:

(11) A self‐propagating, barcoded transposon system for the dynamic rewiring of genomic networksMolecular Systems BiologyJune 12, 2023Max A English, Miguel A Alcantar, James J CollinsStandard Buffer,New England Biolabs,M0484L Q5® Hot Start High‐Fidelity 2X Master Mix,New England Biolabs,M0494L BbsI‐HF,New England Biolabs,R3539L BsmBI‐v2,New England Biolabs,R0739L FseI,New England Biolabs,R0588L SbfI‐HF,New England Biolabs

(12) The Pathfinder plasmid toolkit for genetically engineering newly isolated bacteria enables the study of Drosophila-colonizing OrbaceaeISME CommunicationsMay 24, 2023Katherine M. Elston, Laila E. Phillips, Sean P. Leonard, Eleanor Young, Jo-anne C. Holley, Tasneem Ahsanullah, Braydin McReynolds, Nancy A. Moran, Jeffrey E. BarrickIntegrated DNA Technologies and then amplified PCR products using either Q5 Hot-Start Master Mix (#M0494S, New England Biolabs) or KOD XL (#71087-3, Millipore Sigma) according to manufacturer’s instructions.

(13) The ATP Synthase γ Subunit ATPC1 Regulates RNA Editing in ChloroplastsInternational Journal of Molecular SciencesMay 24, 2023Jia Ni, Wenjian Song, Nadia Ahmed Ali, Yayi Zhang, Jiani Xing, Kexing Su, Xingxing Sun, Xiaobo Zhaocoding sequence of the 3×FLAG tag was amplified by PCR using Q5 Hot Start High-Fidelity 2×Master Mix (M0494S, NEB, Ipswich, MA, USA). The following thermal condition was used: 98 °C for 3 min, 33 cycles of 98

(14) A new method to synthesize multiple gRNA libraries and functional mapping of mammalian H3K4me3 regionsNucleic Acids ResearchMay 22, 2023Chen Pan, Ran Li, Liyan Shui, Zhengyun Xiao, Yali Wang, Jing Zhu, Chao Wu, Long Zhang, Junling Jia, Min Zhengdesigned via NCBI Primer BLAST, and then PCR was conducted with Q5 Hot Start High-Fidelity 2× Mix (NEB, M0494) (Supplementary Table S11). The PCR product was purified via a QIAquick PCR Clean-up Kit and was constructed

(15) HPV integration generates a cellular super-enhancer which functions as ecDNA to regulate genome-wide transcriptionNucleic Acids ResearchMay 22, 2023Rui Tian, Zheying Huang, Lifang Li, Jingping Yuan, Qinghua Zhang, Lirong Meng, Bin Lang, Yongfeng Hong, Chaoyue Zhong, Xun Tian, et al.polymerase chain reaction (PCR) was conducted using the Q5 Hot Start High-Fidelity 2X Master Mix (NEB, M0494S). The primers are listed in Supplementary Table S3. RT–qPCR Total RNA was extracted by RNA-easy Isolation

(16) High-resolution spatial multi-omics reveals cell-type specific nuclear compartmentsbioRxivMay 12, 2023Yodai Takei, Yujing Yang, Jonathan White, Jina Yun, Meera Prasad, Lincoln J Ombelets, Simone Schindler, Long Caifrom 13 different oligo array pools. Briefly, we performed PCR with Q5 Hot Start High-Fidelity (NEB M0494S), column purification with QIAquick PCR Purification Kit (Qiagen 28104), in vitro transcription (NEB

(17) DNA rehybridization drives product release from Cas9 ribonucleoprotein to enable multiple-turnover cleavageNucleic Acids ResearchMay 8, 2023Juan Pan, Megumu Mabuchi, Gregory Brett RobbMonarch® Total RNA Miniprep Kit (#T2010S), Monarch® PCR & DNA Cleanup Kit (#T1030L), Q5® Hot Start High-Fidelity 2× Master Mix (#M0494L), EcoRI-HF (#R3101S), rSAP (#M0371S), T4 DNA ligase (#M0202S), NEB® 10-beta Competent Escherichia coli

(18) Improving the sensitivity of in vivo CRISPR off-target detection with DISCOVER-SeqNature methodsMay 1, 2023Zou RS, Liu Y, Gaido OER, Konig MF, Mog BJ, Shen LL, Aviles-Vazquez F, Marin-Gonzalez A, Ha T.samples were amplified with PCR using Q5 HotStart High-Fidelity 2X Master Mix (New England BioLabs, M0494). Primer pairs for all sequences are listed in Supplementary Table 3. For example, the primer set for

(19) Improved cytosine base editors generated from TadA variantsNature BiotechnologyMay 1, 2023Dieter K. Lam, Patricia R. Feliciano, Amena Arif, Tanggis Bohnuud, Thomas P. Fernandez, Jason M. Gehrke, Phil Grayson, Kin D. Lee, Manuel A. Ortega, Courtney Sawyer, et al.Multiplex PCR Master Mix (Thermo Fisher Scientific, F564L), Q5 Hot Start High-Fidelity 2X Master Mix (NEB, M0494L), Mach T1 competent cells (Thermo Fisher Scientific, C8681201) and ZymoPURE II Plasmid Midiprep kits

(20) Late Embryogenesis Abundant Proteins Contribute to the Resistance of Toxoplasma gondii Oocysts against Environmental StressesmBioApril 25, 2023David Arranz-Solís, David Warschkau, Benedikt T. Fabian, Frank Seeber, Jeroen P. J. Saeijconducted by using either Q5 Hot Start High-Fidelity DNA polymerase (New England Biolabs, cat. no. M0494S) for cloning purposes, or MangoMix (Meridian Bioscience ref. BIO-25034) for diagnostic/screening purposes.

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(1)

10/6/2023Convenient PCR Master Mix From New England Biolabs Anh NguyenI used this Master Mix for running PCR which contained all the enzymes and reagents needed. I only needed to add primers, a DNA template, and water to this Master Mix for PCR reactions.
(1)

3/31/2023Good PCR Master Mix YONG MTry this master mix for hard PCR application from genomic DNA. It's simple to use and the mix includes every component. The enzyme has high fidelity and could amplify the difficult fragment.
(1)

1/2/2020DNA Insert Amplification for CloningDr. Thippeswamy SannasiddappaHighly recommended for amplifying error free longer length DNA inserts. Also, reactions can be set up room temperature.

Q5 Hot Start High-Fidelity 2X Master Mix

Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs

New England Biolabs

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Q5 Hot Start High-Fidelity 2X Master Mix from New England Biolabs

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