Description
Short Description: Truncated human Histone H3.2 is produced in E Coli and purified using FPLC. The purified protein is subsequently ligated to an N-terminal histone tail peptide containing a monomethyl lysine 4 via a native peptide bond. The full-length protein is then repurified prior to lyophilization. Protein concentration was determined using the molar extinction coefficient for Histone H3 and absorbance at 280nm. The molecular weight is 15,239 Daltons. Recombinant histones are suitable for use as positive controls in the analysis of histone post-translational modifications, as substrates for histone modification enzymes, or to generate chromatin in vitro