Description
Recombinant 2019-nCoV Spike protein S1 subunit, RBD (K417N)(319-541) was expressed in CHO cells using a C-terminal his tag. The gene accession number is MN908947.
The receptor binding domain (RBD) of the SARS-CoV-2 spike glycoprotein recognizes the host ACE2 receptor and is a major determinant of viral entry and neutralization (1). K417N mutation in the RBD region of spike protein has been identified in B.1.351 lineages. In addition to antigenic effect, K417N alters binding affinity to ACE-2 protein. As new variants displace the first-wave virus, it is pivotal to evaluate their transmissibility, virulence and their possible tendency to escape antibody neutralization (2, 3)