Description
Normal human plasma clots are dissociated using non-denaturing conditions in which non fibrin bound plasma proteins are removed. The soluble human fibrin clot is then stored under acidic conditions. The soluble human fibrin will reassemble into an insoluble clot when pH is restored to neutral (e.g. by adding one-tenth volume of 10X PBS to the soluble fibrin). SDS-PAGE shows partially crosslinked fibrin with no observable free alpha, beta or gamma chain subunits. No detectable thrombin or plasmin activity is observed in the final product. Labeling efficiency was measured in 0.1M HEPES; 0.1M NaCl; 0.1% SDS; pH 7.4