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EnGenMutation Detection Kit
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EnGenMutation Detection Kit
EnGenMutation Detection Kit from New England Biolabs
(1)
Write a review
Citations:
(6)
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EnGenMutation Detection Kit
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Description
EnGen Mutation Detection Kit - 25 rxns
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
EnGenMutation Detection Kit
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
E3321S
Quantity
25 reactions
Applications
Functional Genomics
Brand/Trademark
New England Biolabs Inc (NEB)
Temperature
-20 C
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New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (6)
(1)
Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24
iScience
July 21, 2023
Patel LR, Stratton SA, McLaughlin M, Krause P, Allton K, Rivas AL, Barbosa D, Hart T, Barton MC.
T7E1
assays
were performed using the EnGen® Mutation Detection Kit per manufacturers protocol (
NEB
E3321S
) and gene specific primers to amplify a ∼500bp region containing PAM site targeted by Synthego to perform
(2)
Membrane-Tethered Mucin 1 Is Stimulated by Interferon and Virus Infection in Multiple Cell Types and Inhibits Influenza A Virus Infection in Human Airway Epithelium
mBio
August 30, 2022
Iverson E, Griswold K, Song D, Gagliardi TB, Hamidzadeh K, Kesimer M, Sinha S, Perry M, Duncan GA, Scull MA.
Sorted transduced cells were frozen for later use or analyzed with an EnGen mutation detection kit (no.
E3321S
,
New England BioLabs
) for on-target
gene editing
confirmation (forward primer, 5′-AGC ACT TCT CCC CAG
(3)
Flow Assisted Mutation Enrichment (FAME): A highly efficacious and efficient method to enrich Double Knockouts (DKO) after gene editing
PLOS ONE
March 4, 2021
Michael Hansen, Xiaopin Cai, Sara Bowen, David A. Largaespada, Ming V. Li
(T7EI)
assay
The T7EI
assay
was conducted with kits purchased from
New England Biolabs
(Catalog #
E3321S
), according to manufacturer’s instruction. Briefly,
PCR
was conducted with provided high-fidelity polymerase
(4)
Development of a CRISPR-SaCas9 system for projection- and function-specific gene editing in the rat brain
Science Advances
March 20, 2020
Haojie Sun et al.
sequence was amplified with
polymerase chain reaction (PCR
) by Q5 Hot Start High-Fidelity 2× Master Mix (
E3321S
,
New England Biolabs
) with the following program: preheat at 98°C for 30 s, 35 cycles of three-step
(5)
Niche stiffness underlies the ageing of central nervous system progenitor cells
Nature
September 5, 2019
Michael Segel et al.
28104
) and the surveyor
assay
was performed as directed from the Engen Mutation Detection Kit (
NEB
;
E3321S
). Samples were subsequently run on a 2% agarose gel and band intensity was measured using ImageJ. For
(6)
Sox9
regulates cell state and activity of embryonic mouse mammary progenitor cells
Communications biology
January 1, 2018
Kogata N, Bland P, Tsang M, Oliemuller E, Lowe A, Howard BA.
QuickExtract DNA Extraction Solution (QE0905T, Qiagen, Peterborough, UK) and EnGen™ Mutation Detection Kit (
E3321S
,
New England Biolabs
, Herts, UK) according to the manufacturer’s instructions. We tested four different
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Reviews
Write a Review
(1)
12/2/2022
Good Kit for Measuring Editing Efficiency
Dimi Comfu
We were interested in studying the role of DNA methylation in the differentiation of of CD4 T-cells. CRISPR-Cas9 approaches were used to knock-out epigenetic enzymes in primary cells and cell lines. ...
Read Review
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EnGenMutation Detection Kit from New England Biolabs
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