SrfI R0629S from New England Biolabs

Product Specs
ItemSrfI
CompanyNew England Biolabs
Price $85.00 Inquire
Catalog NumberR0629S
Size500 units
ApplicationsDNA Manipulation
TypeRestriction Enzyme
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Insights into pegRNA design from editing of the cardiomyopathy‐associated phospholamban R14del mutationFEBS LettersSeptember 1, 2025Bing Yao, Qiangbing Yang, Christian J.B. Snijders Blok, Mark A. Daniels, Pieter A. Doevendans, Raymond Schiffelers, Joost P.G. Sluijter, Zhiyong Lei[16]. This plasmid was cleaved directly in the middle of the eGFP and mCherry sequences with SrfI (#R0629S; New England Biolabs, Ipswich, MA, USA), for 2 h at 37 °C, creating a blunt end, after which the 10

(2) lncRNA 1700009J07Rik Impaired Male Fertility by Interfering with Sexual Behaviors in MiceInt J Mol SciJune 17, 2025Hongyu Wang, Xiaojun Liu, Shijue Dong, Yang Zhou, Jingyan Yu, Meng Zou, Mengqian Ding, Aiwen Kang, Nanxi Ji, Xuhui Zeng, et al.individually. The plasmid with the target sequence was cut by a pair of restriction enzymes, SrfI (R0629S) and SacII (R0157S) (New England Biolabs, Ipswich, MA, USA), and was then ligated to the purified Flag-MCS-IRES-GFP-pcDNA3.1(-)-(Amp+)

(3) PAM‐relaxed and temperature‐tolerant CRISPR‐Mb3Cas12a single transcript unit systems for efficient singular and multiplexed genome editing in rice, maize, and tomatoPlant Biotechnology JournalJanuary 1, 2025Shishi Liu, Yao He, Tingting Fan, Meirui Zhu, Caiyan Qi, Yanqin Ma, Mengqiao Yang, Liang Yang, Xu Tang, Jianping Zhou, et al.synthesized by Sangon biotech (Shanghai, China) and then cloned into pLSS375‐SbfI + SrfI (NEB, catalogue R0629S) using Gibson assembly. The NLS sequences were provided in Figure S13. The DR sequences (4n61, 4n91,

(4) A simple method for rapid cloning of complete herpesvirus genomesCell Reports MethodsFebruary 26, 2024Jan Knickmann, Laura Staliunaite, Olha Puhach, Eleonore Ostermann, Thomas Günther, Jenna Nichols, Michael A. Jarvis, Sebastian Voigt, Adam Grundhoff, Andrew J. Davison, et al.FD0274, FD1194, FD1344, FD0394, FD0514, FD0504, FD0754" Restriction enzyme SrfI,New England Biolabs,Cat#R0629S Zymolyase 20T from Arthrobacter luteus,MP Biomedicals,Cat#08320921 SORB/SD/-His with agar,Takara Bio,Cat#630313 SORB/SD/-His

(5) Chimpanzee adenovirus-mediated multiple gene therapy for age-related macular degenerationiScienceOctober 20, 2023Selena Wei-Zhang, Bohao Cui, Man Xing, Jiaojiao Liu, Yingying Guo, Kai He, Tinghui Bai, Xue Dong, Yi Lei, Wei Zhou, et al.recombinant proteins","Chemicals, peptides, and recombinant proteins" ,, SrfI,New England Biolabs,Cat#R0629S PI-SceI,New England Biolabs,Cat#R0696S I-CeuI,New England Biolabs,Cat#R0699S PacI,New England Biolabs,Cat#R0547L ApaI,New England Biolabs

(6) Enhanced fluorescent imaging of proteins in live yeast cells using fluorescently labeled scFvSTAR ProtocolsJune 2, 2023Ioannis Tsirkas, Tomer Zur, Daniel Dovrat, Zohar Paleiov, Lior Ravkaie, Amir Aharoni(powder),GoldBio,Cat# N-500-3 Water (H2O),Sigma-Aldrich,Cat# 7732-18-5 SrfI + rCutsmart,New England Biolabs,Cat# R0629S PacI + rCutsmart,New England Biolabs,Cat# R0547S Agarose (powder),Life Gene,Cat# LAG0701 SYBR Safe DNA Gel Stain

(7) Characterization of antibiotic resistomes by reprogrammed bacteriophage-enabled functional metagenomics in clinical strainsNature MicrobiologyFebruary 9, 2023Gábor Apjok, Mónika Számel, Chryso Christodoulou, Viktória Seregi, Bálint Márk Vásárhelyi, Tamás Stirling, Bálint Eszenyi, Tóbiás Sári, Fanni Vidovics, Erika Nagrand, et al.quantified. Next, the plasmid mixture was linearized by adding 5 U of SrfI restriction endonuclease (NEB, R0629S) for every 1 µg of plasmid DNA (one h at 37 °C, followed by inactivation at 65 °C for 20 min), and DNA

(8) A standard for near-scarless plasmid construction using reusable DNA partsNature CommunicationsDecember 1, 2019Xiaoqiang Ma, Hong Liang, Xiaoyi Cui, Yurou Liu, Hongyuan Lu, Wenbo Ning, Nga Yu Poon, Benjamin Ho, Kang Zhou(Supplementary Fig. 14d). The RE-digestion solution contained 1 µL of each RE (NotI [R3189S, NEB] and/or SrfI [R0629S, NEB]), a certain quantity of plasmid (~1 µg, PGTP1 or PGTP2), 5 µL of 10XCutSmart® Buffer, and ultrapure

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SrfI

SrfI from New England Biolabs

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New England Biolabs

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SrfI from New England Biolabs

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