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FITC Annexin V
FITC Annexin V from BioLegend
(1)
Write a review
Citations:
(249)
Supplier Page
Supplier Page from
BioLegend for
FITC Annexin V
Description
FITC Annexin V
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
FITC Annexin V
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
640906
Applications
Flow Cytometry (FC)
Quantity
100 tests
Gene Name
annexin A5
NCBI Gene Aliases
ANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
NCBI Full Gene Name
annexin A5
Format
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Target
Annexin V
Species
Human, Mouse, Rat
Conjugate/Tag
FITC
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (249)
(1)
Pyruvate anaplerosis is a targetable vulnerability in persistent leukaemic stem cells
Nature communications
August 17, 2023
Rattigan KM, Brabcova Z, Sarnello D, Zarou MM, Roy K, Kwan R, de Beauchamp L, Dawson A, Ianniciello A, Khalaf A,
et al
.
CD34 analysis Cells were stained with Annexin V (fluorescein isothiocyanate (FITC,
BioLegend
: Cat#
640906
, 5 µL/test),
7-AAD
(
BD Bioscience
: Cat#
559925
, 5 µL/test) and
CD34
+ (APC,
BD Bioscience
,
clone
581,
(2)
MTH1 protects platelet mitochondria from oxidative damage and regulates platelet function and thrombosis
Nature communications
August 10, 2023
Ding Y, Gui X, Chu X, Sun Y, Zhang S, Tong H, Ju W, Li Y, Sun Z, Xu M,
et al
.
with CRP (5 μg/ml) or thrombin (0.5 U/ml) for 30 min and then stained with
FITC-Annexin-V
(
Biolegend
,
640906
,
1:20
) for 15 min followed by detecting Annexin-V binding by
flow cytometry
. Annexin-V binding was presented
(3)
Nuclear morphology predicts cell survival to cisplatin chemotherapy
Neoplasia
August 1, 2023
Chi-Ju Kim, Anna LK Gonye, Kevin Truskowski, Cheng-Fan Lee, Yoon-Kyoung Cho, Robert H. Austin, Kenneth J. Pienta, Sarah R. Amend
orange
channels.
Flow cytometry analysis
Live cells were incubated with a
FITC-Annexin V
(
BioLegend
, #
640906
) according to the manufacturer's recommendations. Cells were analyzed on an
Attune NxT Flow Cytometer
(4)
In silico enhancer mining reveals SNS-032 and EHMT2 inhibitors as therapeutic candidates in high-grade serous ovarian cancer
British Journal of Cancer
July 27, 2023
Marcos Quintela, David W. James, Jetzabel Garcia, Kadie Edwards, Lavinia Margarit, Nagindra Das, Kerryn Lutchman-Singh, Amy L. Beynon, Inmaculada Rioja, Rab K. Prinjha,
et al.
bars represent the coefficient of variation. Apoptosis
staining
was performed using Annexin V-FITC (
640906
,
Biolegend
; CA, US) and Propidium iodide (PI,
421301
,
Biolegend
) as per manufacturer’s instructions,
(5)
EHBP1L1, an apicobasal polarity regulator, is critical for nuclear polarization during enucleation of erythroblasts
Blood Advances
July 25, 2023
Ji Wu, Kenta Moriwaki, Tatsuya Asuka, Ritsuko Nakai, Satoshi Kanda, Manabu Taniguchi, Tatsuki Sugiyama, Shin-ichiro Yoshimura, Masataka Kunii, Takashi Nagasawa,
et al.
N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid, 140 mM NaCl, and 2.5 mM CaCl2) and then stained with FITC-conjugated Annexin V (
BioLegend
,
640906
). The fluorescently labeled antibodies are listed in supplemental Table 1. For intracellular
staining
(6)
Optogenetic Induction of Pyroptosis, Necroptosis, and Apoptosis in Mammalian Cell Lines
BIO-PROTOCOL
July 20, 2023
Kateryna Shkarina, Petr Broz
424001
)Annexin V Pacific Blue (BioLegend, catalog number:
640918
)Annexin V FITC (
BioLegend
, catalog number:
640906
)Annexin V Alexa Fluor 647 (
BioLegend
, catalog number:
640912
)CellEvent
caspase-3
/7 green (
Thermo Fisher Scientific
(7)
Metabolic Profiles Point Out Metabolic Pathways Pivotal in Two Glioblastoma (GBM) Cell Lines, U251 and U-87MG
Biomedicines
July 20, 2023
Filipa Martins, David van der Kellen, Luís G. Gonçalves, Jacinta Serpa
Then, cell pellets were incubated with 0.5 µL annexin V-fluorescein isothiocyanate (annexin V-FITC;
640906
,
BioLegend
, San Diego, CA, USA), in
annexin V binding buffer
1× (10 mM
HEPES
(pH 7.4; 391333,
Millipore
,
(8)
A localized hydrogel-mediated chemotherapy causes immunogenic cell death via activation of ceramide-mediated unfolded protein response
Science advances
June 30, 2023
Kar A, Jain D, Kumar S, Rajput K, Pal S, Rana K, Kar R, Jha SK, Medatwal N, Yavvari PS,
et al
.
Allprotect tissue reagent (#
76405
) was purchased from
QIAGEN
, Hilden, Germany. Annexin-FITC
assay
kit (#
640906
) was purchased from
BioLegend
, San Diego, USA. Developer solution (#4908216) and XBT
x-ray
film (#6568307)
(9)
miR-216b-5p promotes late apoptosis/necroptosis in trastuzumab-resistant SK-BR-3 cells
Turkish Journal of Biology
May 23, 2023
İŞTAR BURCU DOLAPÇI, SENEM NOYAN, AYŞEGÜL YÜCEL POLAT, HAKAN GÜRDAL, BALA GÜR DEDEOĞLU
pH: 7.4; 140 mM NaCl, 2.5 mM CaCl2) in a concentration of 1 × 106 cells/mL. Five μL Annexin V-FITC (
640906
,
BioLegend
) and five μL PI (
421301
,
BioLegend
) (0.5 μg/μL) were added and the cells were incubated in
(10)
The synthetic oleanane triterpenoid CDDO-2P-Im binds GRP78/BiP to induce unfolded protein response-mediated apoptosis in myeloma
Molecular oncology
May 7, 2023
Luo G, Aldridge K, Chen T, Aslot V, Kim BG, Han EH, Singh N, Li S, Xiao TS, Sporn MB,
et al
.
DMSO (1%) or the specified therapeutic agents for 24 h. Cells were stained with Annexin V (Catalog #
640906
;
Biolegend
, San Diego, CA, USA) and propidium iodide (Catalog #
421301
;
Biolegend
) after 24 h and analyzed
(11)
Caspase-8 is required for HSV-1-induced apoptosis and promotes effective viral particle release via autophagy inhibition
Cell death and differentiation
April 1, 2023
Marino-Merlo F, Klett A, Papaianni E, Drago SFA, Macchi B, Rincón MG, Andreola F, Serafino A, Grelli S, Mastino A,
et al
.
Sigma-Aldrich
(Taufkirchen, Germany).
FITC-Annexin-V
was bought from
BioLegend
(San Diego, CA, USA, #
640906
) and the p38 MAPK inhibitor SB203580 from Calbiochem (San Diego, CA, USA). Recombinant CD95/FasL was
(12)
Targeting Tn-positive tumors with an afucosylated recombinant anti-Tn IgG
Scientific Reports
March 28, 2023
Yasuyuki Matsumoto, Nan Jia, Jamie Heimburg-Molinaro, Richard D. Cummings
°C in CO2 incubator. Cells were resuspended and co-stained with FITC-labeled Annexin V antibody (Cat#
640906
,
BioLegend
, diluted at
1:20
) and 1 μg/mL of propidium iodide (PI) (Cat#
P3566
,
Invitrogen
) in Annexin
(13)
Use of phage display biopanning as a tool to design CAR-T cells against glioma stem cells
Frontiers in Oncology
March 24, 2023
Marine Potez, Sebastian Snedal, Chunhua She, Jongmyung Kim, Konrad Thorner, Timothy H. Tran, Maria Cecilia Ramello, Daniel Abate-Daga, James K. C. Liu
minutes at 4°C in the dark. Cells were then washed, secondarily stained with Annexin-V-FITC (
Biolegend
,
640906
) and
DAPI
, incubated for 15 minutes at room temperature in the dark, and then analyzed. Data acquisition
(14)
The poly(C)-binding protein Pcbp2 is essential for CD4+ T cell activation and proliferation
iScience
January 20, 2023
Massimo Martinelli, Gabrielle Aguilar, David S.M. Lee, Andrew Kromer, Nhu Nguyen, Benjamin J. Wilkins, Tatiana Akimova, Ulf H. Beier, Louis R. Ghanem
Flow cytometric
detection of apoptosis and
necrosis
was performed with FITC–labeled Annexin V (cat. #
640906
,
BioLegend
) and the live/dead fixable aqua dead
cell stain
kit (cat. #
L34957
;
Thermofisher Scientific
).
T cell
(15)
Large‐scale genetic screens identify BET ‐1 as a cytoskeleton regulator promoting actin function and life span
Aging Cell
January 1, 2023
Gilberto Garcia, Raz Bar‐Ziv, Maxim Averbukh, Nirmalya Dasgupta, Naibedya Dutta, Hanlin Zhang, Wudi Fan, Darius Moaddeli, C. Kimberly Tsui, Toni Castro Torres,
et al.
treatment with 30 mM H2O2 was used as a positive control for cell death. Annexin V FITC (
Biolegend
,
640906
) and live/dead Aqua (
ThermoFisher
,
L34966
)
staining
was performed as per manufacturer's protocol. Briefly,
(16)
Impaired CXCL12 signaling contributes to resistance of pancreatic cancer subpopulations to T cell-mediated cytotoxicity
OncoImmunology
December 31, 2022
Yuan-Na Lin, Marcel O. Schmidt, Ghada M. Sharif, Eveline E. Vietsch, Amber J. Kiliti, Megan E. Barefoot, Anna T. Riegel, Anton Wellstein
(17)
Precursor‐Directed Synthesis of Apoptosis‐Initiating N ‐Hydroxyalkyl Phenylbenzoisoquinolindione Alkaloids
ChemistryOpen
December 7, 2022
Yu Chen, Hans‐Martin Dahse, Christian Paetz, Bernd Schneider
apoptotic cells by
flow cytometry
, the described protocols for Annexin V FITC (
Biolegend
, cat. no.
640906
) and Fixable Viability Dye eFluor780 (
eBioscience
, cat. no.
65–0865‐14
) were used. Supporting Information
(18)
STING agonism reprograms tumor-associated macrophages and overcomes resistance to PARP inhibition in BRCA1-deficient models of breast cancer
Nature Communications
December 1, 2022
Qiwei Wang et al.
treatment) were washed twice with cold PBS, and then incubated with 5 μL
FITC Annexin V
(
BioLegend
, #
640906
) and 10 μL 7-AAD (
BioLegend
, #
420404
) in 100 μL
Annexin V binding buffer
(
BioLegend
, #
422201
) at room
(19)
MEK and MCL-1 sequential inhibition synergize to enhance rhabdomyosarcoma treatment
Cell Death Discovery
December 1, 2022
Clara Alcon et al.
h of
incubation
with the specified treatments, cells were analyzed using Annexin V (FITC Annexin V,
640906
,
BioLegend
) and propidium iodide (PI) (1056, BioVision, Milpitas, California, USA) and analyzed on a
(20)
The 3D enhancer network of the developing T cell genome is shaped by SATB1
Nature communications
November 14, 2022
Zelenka T, Klonizakis A, Tsoukatou D, Papamatheakis DA, Franzenburg S, Tzerpos P, Tzonevrakis IR, Papadogkonas G, Kapsetaki M, Nikolaou C,
et al
.
were anti-DNA PI-conjugated (Biolegend, 79997), anti-Phosphatidylserine FITC conjugated (
Biolegend
,
640906
, 1/100), anti-CD4 PE-conjugated (
Pharmingen
,
553730
, 1/100), anti-
CD8a
APC conjugated (
Biolegend
,
100712
,
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Reviews
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1/6/2015
Measurement of Differential Apoptosis of HEK cells in presence of PDGF
Anirban Banerjee
Apoptosis of keratinocytes is a crucial step that balances proliferation to maintain proper epidermal thickness. However keratinocytes from the peripheral area of chronic wounds undergo higher and ...
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FITC Annexin V from BioLegend
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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