Collagenase, Type I, powder 17100017 from Thermo Fisher Scientific

Product Specs
ItemCollagenase, Type I, powder
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number17100017
Quantity1g
ApplicationsCELL CULTURE
Brand/TrademarkGibco™
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures

Specifications/Features

Collagenase

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Autophagy critically controls skin inflammation and apoptosis-induced stem cell activationAutophagyNovember 2, 2023Lisette Van Hove, Annagiada Toniolo, Mohammad Ghiasloo, Kim Lecomte, Fleur Boone, Maarten Ciers, Kris Raaijmakers, Niels Vandamme, Jana Roels, Sophia Maschalidi, et al.digestion for 1 hour at 37°C and subsequent digestion with collagenase type 1 (1.25 mg/ml, Invitrogen, 17100017), type 2 (0.5 mg/ml; Sigma, C2–22) and type 4 (0.5 mg/ml; Sigma, C5138) for 45 min. Live cells were

(2) DNA damage repair profiling of esophageal squamous cell carcinoma uncovers clinically relevant molecular subtypes with distinct prognoses and therapeutic vulnerabilitieseBioMedicineOctober 1, 2023Ning Zhao, Zicheng Zhang, Qiang Wang, Lin Li, Zichao Wei, Hongyan Chen, Meng Zhou, Zhihua Liu, Jianzhong Suisotype control. Tumor tissues were dissected, minced and digested with 5 mg/ml collagenase Type I (17100017; ThermoFisher) at 37 °C for 30 min. The isolated cells were passed through a 70 μm filter, centrifuged

(3) Establishment and Characterization of Continuous Satellite Muscle Cells from Olive Flounder (Paralichthys olivaceus): Isolation, Culture Conditions, and Myogenic Protein ExpressionCellsSeptember 21, 2023Sathish Krishnan, Selvakumari Ulagesan, Josel Cadangin, Ji-Hye Lee, Taek-Jeong Nam, Youn-Hee Choiskeletal muscle was enzymatically digested using 10 mL of 5 mg/mL collagenase type IV (Gibco; lot number:17100017) solution and incubated in a sterile 50 mL conical tube for 45 min with rotation at 200 rpm at 4 °C.

(4) Short heat shock has a long-term effect on mesenchymal stem cells’ transcriptomeiScienceAugust 18, 2023Ivana Ribarski-Chorev, Gisele Schudy, Carmit Strauss, Sharon Schlesingeracid-2-phosphate,Sigma,Cat# A8960 Bodipy 493/503,Invitrogen,Cat# D3922 Collagenase Type I,Invitrogen,Cat# 17100017 Collagenase Type II,Sigma,Cat# C6885 Dexamethasone,Sigma,Cat# D4902 DMEM low glucose,Gibco or Biological

(5) Characterization of a Decellularized Sheep Pulmonary Heart Valves and Analysis of Their Capability as a Xenograft Initial Matrix Material in Heart Valve Tissue EngineeringBioengineering (Basel)August 9, 2023Müslüm Süleyman İnal, Cihan Darcan, Ali Akpekweight (W0) of each sample was determined after lyophilization (Labart, LFD-10N). Collagenase I (Gibco-17100017) solution was prepared in PBS buffer at a concentration of 1 mg/mL (125 U/mL). A total of 1 mL of collagenase

(6) Neuroprotective protein ADNP-dependent histone remodeling complex promotes T helper 2 immune cell differentiationImmunityJuly 11, 2023Ferreira ACF, Szeto ACH, Clark PA, Crisp A, Kozik P, Jolin HE, McKenzie ANJ.carrier-free",Biolegend,Cat#574306 "rmIL-12, carrier-free",Biolegend,Cat#577006 PMSF,Sigma Aldrich,Cat#P7626 collagenase I,Gibco,Cat#17100017 "DNase I, from bovine pancreas",Sigma-Aldrich,Cat#D5025/DN25 Percoll,GE Healthcare,Cat#17-0891-01 cOmplete

(7) Proline hydroxylase 2 (PHD2) promotes brown adipose thermogenesis by enhancing the hydroxylation of UCP1Molecular metabolismJuly 1, 2023Li F, Zhang F, Yi X, Quan LL, Yang X, Yin C, Ma Z, Wu R, Zhao W, Ling M, et al.with modification. BAT was minced into pieces with scissors and digested using collagenase I (2 mg/ml, 17100017, Gibco, containing 3 mM CaCl2) at 37 °C water bath for 30 min. Tissue homogenates were filtered through

(8) Dynamic expression of IGFBP3 modulate dual actions of mineralization micro-environment during tooth development via Wnt/beta-catenin signaling pathwayBiology DirectJune 26, 2023MengDan Zhang, Junming Zheng, Siyuan Wu, Hailing Chen, Lusai XiangHDPSCs were isolated from dental papilla tissue that digested with 3 mg/mL collagenase I (cat. no. 17100017; Gibco) for 30 min at 37 °C. Isolated hDPSCs were cultured with basal media, which contains alpha modified

(9) Disruption of Prostaglandin F(2α) Receptor Signaling Attenuates Fibrotic Remodeling and Alters Fibroblast Population Dynamics in A Preclinical Murine Model of Idiopathic Pulmonary FibrosisbioRxiv : the preprint server for biologyJune 7, 2023Rodriguez LR, Tang SY, Barboza WR, Murthy A, Tomer Y, Cai TQ, Iyer S, Chavez K, Das US, Ghosh S, et al.were digested in Phosphate Buffered Saline (Mg and Ca free) with 2 mg/ml Collagenase Type I (Gibco Cat 17100017) and 50 units of DNase (Millipore Sigma Cat D5025), passed through 70/mum nylon mesh to obtain single-cell

(10) METTL3-dependent m(6)A methylation facilitates uterine receptivity and female fertility via balancing estrogen and progesterone signalingCell death & diseaseJune 3, 2023Wan S, Sun Y, Zong J, Meng W, Yan J, Chen K, Wang S, Guo D, Xiao Z, Zhou Q, et al.epithelial cells. And the remaining tissues were further digested within 0.15 mg/ml collagenase I (17100017, Invitrogen) in HBSS at 37 °C for 30 min. The tissues were washed 3 times with HBSS. And the supernatant

(11) Accumulated cholesterol protects tumours from elevated lipid peroxidation in the microenvironmentRedox biologyJune 1, 2023Zhao X, Lian X, Xie J, Liu G.0.5 mm) with scissors. In addition, 10% FBS 1640 media (v/v) containing 1 mg/mL Type I Collagenase (17100017, Gibco) covered the samples at 37 °C for disaggregation. Tumour particles were pipetted vigorously by

(12) Tissue-like cultured fish fillets through a synthetic food pipelinenpj Science of FoodMay 6, 2023Enbo Xu, Ruihao Niu, Jihui Lao, Shengliang Zhang, Jie Li, Yiyuan Zhu, Huimin Shi, Qingqing Zhu, Yijian Chen, Yuyan Jiang, et al.adipose tissue was scraped from the abdomen. Then the tissue was treated with 0.1% type I collagenase (Gibco 17100017) solution to vibrate and digest at room temperature for 1 h. After removing the mature adipocytes and

(13) Intestinal cell type-specific communication networks underlie homeostasis and response to Western dietJournal of Experimental MedicineMay 1, 2023Yu-Chen Wang, Yang Cao, Calvin Pan, Zhiqiang Zhou, Lili Yang, Aldons J. LusisThe extra media was removed and 10 ml RPMI 1640 culture media with 1 mg/ml Collagenase type I (Cat# 17100017; Thermo Fisher Scientific) was added. The sample was incubated for 20 min at 37°C with 250 rpm rotation

(14) High-throughput drug screening identifies fluoxetine as a potential therapeutic agent for neuroendocrine prostate cancerFrontiers in OncologyMarch 13, 2023Lei Chen, Yiyi Ji, Ang Li, Bo Liu, Kai Shen, Ruopeng Su, Zehua Ma, Weiwei Zhang, Qi Wang, Yinjie Zhu, et al.minced, and digested in DMEM culture medium (Gibco, 11965092) containing 0.2mg/mL collagenase (Gibco, 17100017), 0.1mg/mL DNase I (STEMCELL Technologies, 07469), 1U/mL dispase (Sangon, 78990-62-2) and 2% FBS (Gibco,

(15) Medicinal plant-derived mtDNA via nanovesicles induces the cGAS-STING pathway to remold tumor-associated macrophages for tumor regressionJournal of NanobiotechnologyMarch 6, 2023Jinfeng Liu, Jiaxin Xiang, Cuiyuan Jin, Lusha Ye, Lei Wang, Yanan Gao, Nianyin Lv, Junfeng Zhang, Fuping You, Hongzhi Qiao, et al.tumors were excised, minced and digested with 5% FBS DMEM containing 2 mg/mL collagenase I (Gibco, 17100017) and 2 mg/mL hyaluronidase (Sigma-Aldrich, H6254) at 37 °C for 45 min with agitation, followed by treatment

(16) Protocol for cell-type-specific tissue reconstruction in the murine lung fibrogenic microenvironmentSTAR ProtocolsMarch 1, 2023Koichi Hasegawa, Ryo Miyata, Toshi Menju, Toyohiro Hirai, Hiroshi Date, Atsuyasu SatoBalanced Salt Solution (HBSS),Wako Pure Chemical Industries,Cat# 084-08965 Collagenase type I,Gibco,Cat# 17100017 Dispase,Corning,Cat# 354235 Dulbecco’s Modified Eagle Medium (DMEM),Sigma-Aldrich,Cat# D5030 Antibiotic-antimycotic,Life Technologies

(17) Hordenine Activated Dermal Papilla Cells and Promoted Hair Regrowth by Activating Wnt Signaling PathwayNutrientsJanuary 30, 2023Wang C, Zang K, Tang Z, Yang T, Ye X, Dang Y.hair bulb was cut from the lower end of the hair follicle, digested with 0.2% collagenase I (Gibco, 17100017, New York, NY, USA) for 1 h, and filtered with a 40 μm filter to remove the cells. The remaining tissues

(18) The regulation and function of acetylated high-mobility group box 1 during implantation and decidualizationFrontiers in ImmunologyJanuary 25, 2023Yue Li, Si-Ting Chen, Yu-Ying He, Bo Li, Chen Yang, Zhen-Shan Yang, Zeng-Ming Yangepithelial cells were removed by washing, the remaining uteri were incubated with 0.15 mg/ml collagenase I (17100017, Gibco). The isolated stromal cells were cultured in DMEM/ F12 (D2906, Sigma-Aldrich) containing 10%

(19) Transcriptomic profiling reveals sex-specific molecular signatures of adipose endothelial cells under obesogenic conditionsiScienceJanuary 20, 2023Martina Rudnicki, Alexandra Pislaru, Omid Rezvan, Eric Rullman, Aly Fawzy, Emmanuel Nwadozi, Emilie Roudier, Thomas Gustafsson, Tara L. Haasal.55,RRID:SCR_003070 ,, Other,Other,Other ,, "Collagenase, Type I",Thermo Fisher Scientific,Cat#17100017 "Collagenase, Type I",Worthington Biochemicals,Cat#LS004197 Dynabeads™ Antibody Coupling Kit,Thermo Fisher Scientific

(20) Chrysin ameliorates synovitis and fibrosis of osteoarthritic fibroblast-like synoviocytes in rats through PERK/TXNIP/NLRP3 signalingFrontiers in pharmacologyJanuary 1, 2023Ding L, Liao T, Yang N, Wei Y, Xing R, Wu P, Li X, Mao J, Wang P.incubator and rocked every hour in 9.5 mL of digestion solution (DMEM with 1 mg/mL collagenase type I (17100017, Thermo, Waltham, United States) and 1 mg/mL dispase (S25046-1g, Yuanye, Shanghai, China). After filtration,

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11/9/2018Digesting Tumor Tissue Meng SunWe always use the collagenase type I specifically for digesting tumor tissue harvested from mice. We are a cell metabolism and immunology lab; therefore, after digesting the tissues and isolating for ... Read Review

Collagenase, Type I, powder from Thermo Fisher Scientific

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Thermo Fisher Scientific

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Collagenase, Type I, powder from Thermo Fisher Scientific

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