T4 Polynucleotide Kinase M0201L from New England Biolabs

Product Specs
ItemT4 Polynucleotide Kinase
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberM0201L
Format10 u/µl
Quantity2500 units
Molecule NamePolynucleotide Kinase
ActivityOne Richardson unit is defined as the amount of enzyme catalyzing the incorporation of 1 nmole of acid-insoluble [32P] in 30 minutes at 37°C.
ApplicationsDNA Manipulation
Brand/TrademarkNew England Biolabs Inc (NEB)
TypeEnzymes
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Hfq protein and GcvB small RNA tailoring of oppA target mRNA to levels allowing translation activation by MicF small RNA in Escherichia coliRNA BiologyDecember 31, 2023Marie-Claude Carrier, David Lalaouna, Eric Massémethods Reagents Enzymes EcoRI (R3101), BamHI (R3136), SphI (R3182), MscI (R0534), T4 Polynucleotide kinase (M0201), Protoscript II (M0368), calf intestinal phosphatase (M0290), Q5 High fidelity DNA polymerase (M0491),

(2) The Effect of Pseudoknot Base Pairing on Cotranscriptional Structural Switching of the Fluoride RiboswitchbioRxivDecember 5, 2023Laura M Hertz, Elise N White, Konstantin Kuznedelov, Luyi Cheng, Angela M Yu, Rivaan Kakkaramadam, Konstantin Severinov, Alan Chen, Julius B LucksT4 DNA Ligase Reaction Buffer (NEB, #B0202A), 1 μL T4 Ligase (NEB, #M0202L), and 1 μL T4 PNK (NEB, #M0201L) for 30 to 60 min at room temperature. The ligated product was then transformed into NEBTurbo competent

(3) SLC3A2 N-glycosylation and Golgi remodeling regulate SLC7A amino acid exchangers and stress mitigationJournal of Biological ChemistryDecember 1, 2023Cunjie Zhang, Massiullah Shafaq-Zadah, Judy Pawling, Geoffrey G. Hesketh, Estelle Dransart, Karina Pacholczyk, Joseph Longo, Anne-Claude Gingras, Linda Z. Penn, Ludger Johannes, et al.CRISPR/CAS9 Reagents: FastDigest BbsI (#FD1014) from Thermo Scientific; T7 DNA ligase (#M0318S), T4 PNK (#M0201S), ATP (#P0756S) from NEB; Plasmid-Safe ATP-Dependent DNase (#E3101K) from Epicentre; PolyJet reagent

(4) Rfoot‐seq: Transcriptomic RNase Footprinting for Mapping Stable RNA‐Protein Complexes and Rapid Ribosome ProfilingCurrent ProtocolsNovember 20, 2023Qianru Li, Emily K. Stroup, Zhe JiT4 polynucleotide kinase reaction buffer (New England BioLabs, cat. no. B0201S) T4 Polynucleotide kinase (New England BioLabs, cat. no. M0201L). SUPERase•In RNase inhibitor (Invitrogen, cat. no. AM2694) 5× first strand buffer (Invitrogen, cat.

(5) Synthetic chromosome fusion: Effects on mitotic and meiotic genome structure and functionCell GenomicsNovember 8, 2023Jingchuan Luo, Luis A. Vale-Silva, Adhithi R. Raghavan, Guillaume Mercy, Jonna Heldrich, Xiaoji Sun, Mingyu Kenneth Li, Weimin Zhang, Neta Agmon, Kun Yang, et al.dNTP Mixture (each 2.5 mM),Takara,4030 "T4 Polynucleotide Kinase (10,000 units/ml)",New England BioLabs,M0201S Klenow Fragment,New England BioLabs,M0210S T4 DNA Polymerase,New England BioLabs,M0203S dATP (100 mM

(6) Targeting histone deacetylase suppresses tumor growth through eliciting METTL14-modified m(6) A RNA methylation in ocular melanomaCancer communications (London, England)November 1, 2023Zhuang A, Gu X, Ge T, Wang S, Ge S, Chai P, Jia R, Fan X.washing, the samples on the beads were subjected to dephosphorylation with T4 polynucleotide kinase (M0201L, New England Biolabs, Ipswich, MA, USA) at 37°C for 20 min. The RNA was then purified, subjected to

(7) Integrative genome-scale analyses reveal post-transcriptional signatures of early human small intestinal development in a directed differentiation organoid modelBMC GenomicsOctober 26, 2023Yu-Han Hung, Meghan Capeling, Jonathan W. Villanueva, Matt Kanke, Michael T. Shanahan, Sha Huang, Rebecca Cubitt, Vera D. Rinaldi, John C. Schimenti, Jason R. Spence, et al.Ipswich, MA; M0356), (iv) 5′ end phosphorylation using T4 polynucleotide kinase (NEB, Ipswich, MA; M0201), (iv) 5′ adapter ligation with T4 RNA Ligase 1 (NEB, Ipswich, MA; M0204). The 5’ adaptor contained

(8) Comprehensive quantitative modeling of translation efficiency in a genome‐reduced bacteriumMolecular Systems BiologyOctober 12, 2023Marc Weber, Adrià Sogues, Eva Yus, Raul Burgos, Carolina Gallo, Sira Martínez, Maria Lluch‐Senar, Luis Serranodephosphorylation using Antarctic phosphatase (M0289, New England Biolabs) and rephosphorylation using PNK (M0201, New England Biolabs). Then, 3 prime and 5 prime adapters were ligated to the RNA fragments, and these

(9) The Helix-Loop-Helix motif of human EIF3A regulates translation of proliferative cellular mRNAsPLOS ONESeptember 28, 2023Marina P. Volegova, Cynthia Hermosillo, Jamie H. D. Cateprobe was labeled on the 5′ end with [γ- 32P] ATP (Perkin Elmer) using T4 polynucleotide kinase (NEB M0201) according to the manufacturer’s protocol. Membranes were washed twice by 20 mL 6x SSC for 5 min at

(10) The enhancement of CRISPR/Cas9 gene editing using metforminBiochemistry and Biophysics ReportsSeptember 10, 2023Jaedyn L. Rollins, Raquel M. Hall, Clara J. Lemus, Lauren A. Leisten, Jennifer M. JohnstonNJ). These oligos were annealed in a thermocycler and phosphorylated using PNK (New England Biolabs #M0201L). A single guide RNA sequence specific for the PF4 locus was incorporated into pX330 downstream of the

(11) Large-scale single-cell cloning of genome-edited cultured human cells by On-chip SPiSSTAR ProtocolsSeptember 1, 2023Gou Takahashi, Yuichiro MiyaokaT4 DNA Ligase Reaction Buffer,New England Biolabs,Cat# B0202 T4 Polynucleotide Kinase (T4 PNK),New England Biolabs,Cat# M0201 Ligation High ver.2,Toyobo,Cat# LGK-201 FastAP Thermosensitive Alkaline Phosphatase,Thermo Fisher Scientific

(12) Intermolecular electron transfer in radical SAM enzymes as a new paradigm for reductive activationJournal of Biological ChemistrySeptember 1, 2023Karsten A.S. Eastman, Andrew S. Jochimsen, Vahe Bandarianbuffer (NEB # B0202S), 1 μl of T4 DNA ligase (NEB #M0202S), 1 μl of T4 polynucleotide kinase (NEB #M0201S), and 6 μl of H2O. The mixture was incubated at 20 °C for 2 h to phosphorylate and ligate the new plasmid.

(13) The effect of histone deacetylase inhibitors on the efficiency of the CRISPR/Cas9 systemBiochemistry and Biophysics ReportsSeptember 1, 2023Ymer Björnson, Codey Y. Huang, Jaedyn L. Rollins, Guadalupe Castañeda, Navneet Kaur, Emiko Yamamoto, Jennifer M. Johnstonsynthesized by Integrated DNA Technologies (Newark, NJ) and phosphorylated using PNK (New England Biolabs #M0201L). They were then ligated into a BbsI (New England Biolabs #R0539L) digested pX330 plasmid using T4 DNA Ligase

(14) Structural mechanism of R2D2 and Loqs-PD synergistic modulation on DmDcr-2 oligomersNat CommunAugust 26, 2023Ting Deng, Shichen Su, Xun Yuan, Jinqiu He, Ying Huang, Jinbiao Ma, Jia Wang70% ethanol and air-dried, and the RNA was dissolved in ultrapure water. We next used T4 PNK (NEB, M0201) to remove the 2′,3′ cyclic phosphate at the 3′ end of the RNA. The sense and antisense strands of 19-bp

(15) The extinct Sicilian wolf shows a complex history of isolation and admixture with ancient dogsiScienceAugust 18, 2023Marta Maria Ciucani, Jazmín Ramos-Madrigal, Germán Hernández-Alonso, Alberto Carmagnini, Sabhrina Gita Aninta, Xin Sun, Camilla Hjorth Scharff-Olsen, Liam Thomas Lanigan, Ilaria Fracasso, Cecilie G. Clausen, et al.T4 DNA ligase,New England Biolabs Inc.,Cat#M0202L T4 Polynucleotide Kinase,New England Biolabs Inc.,Cat#M0201L T4 DNA Polymerase,New England Biolabs Inc.,Cat#M0203S "BSt 2,0 warmstart polymerase",New England Biolabs

(16) ATRX guards against aberrant differentiation in mesenchymal progenitor cellsbioRxivAugust 8, 2023Yan Fang, Douglas Barrows, Yakshi Dabas, Thomas S Carroll, William D. Tap, Benjamin A. Nacevphosphatase (NEB, M0290). The oligos were annealed with T4 ligation buffer (NEB, B0202S) with T4 PNK (NEB, M0201L) enzyme. The ligation step was performed using Quickligation buffer (NEB, B2200S) and Quick Ligase (NEB,

(17) Nanopore-based RNA sequencing deciphers the formation, processing, and modification steps of rRNA intermediates in archaeaRNAAugust 1, 2023Felix Grünberger, Michael Jüttner, Robert Knüppel, Sébastien Ferreira-Cerca, Dina Grohmannsequencing RNAs for direct cDNA and PCR-cDNA sequencing were treated with T4 Polynucleotide Kinase (NEB, M0201) following the manufacturer's nonradioactive phosphorylation protocol to dephosphorylate 3′ termini,

(18) METTL1 promotes tumorigenesis through tRNA-derived fragment biogenesis in prostate cancerMolecular CancerJuly 29, 2023Raquel García-Vílchez, Ana M. Añazco-Guenkova, Sabine Dietmann, Judith López, Virginia Morón-Calvente, Silvia D’Ambrosi, Paz Nombela, Kepa Zamacola, Isabel Mendizabal, Saioa García-Longarte, et al.(ThermoFisher Scientific, #88836) were used. After washing, RNA 3’ ends were dephosphorylated using PNK (NEB, M0201L) and FastAP Alkaline phosphatase (ThermoFisher Scientific, EF0654). Next, the 3’ pre-adenylated adapters

(19) Aub, Vasa and Armi localization to phase separated nuage is dispensable for piRNA biogenesis and transposon silencing in DrosophilabioRxivJuly 29, 2023Samantha Ho, Nicholas P Rice, Tianxiong Yu, Zhiping Weng, William E TheurkaufI,NEB,Cat# M0209S T4 DNA polymerase,NEB,Cat# M0203L Klenow DNA polymerase,NEB,Cat# M0210S T4 PNK,NEB,Cat# M0201L Klenow 3ʹ to 5ʹ exo,NEB,Cat# M0212L UDG,NEB,Cat# M0280S Phusion Polymerase,NEB,Cat# M0530S "T4 RNA Ligase 2, truncated

(20) Activation of GPR44 decreases severity of myeloid leukemia via specific targeting of leukemia initiating stem cellsCell reportsJuly 25, 2023Qian F, Nettleford SK, Zhou J, Arner BE, Hall MA, Sharma A, Annageldiyev C, Rossi RM, Tukaramrao DB, Sarkar D, et al.T4 DNA ligase,New England Biolabs,Cat# M0202S T4 Polynucleotide Kinase (500 units),New England Biolabs,Cat# M0201S Torin 1,Selleckchem,Cat# S2827 TransIT®-293 Reagent,MirusBio,Cat# MIR 2705 TRI Reagent®,Sigma Aldrich,Cat#

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4/19/2016T4 Kinase Kit Benjamin GerovacThis kit is used to add 5' phosphate groups to digested DNA so it can be ligated and cloned.

In Vitro Transcription and Immobilized Template Analysis of Preinitiation Complexes
January 01, 2012
In Vitro Selection of RNA Aptamers to a Protein Target by Filter Immobilization
October 01, 2009
Serial Analysis of Gene Expression (SAGE): Experimental Method and Data Analysis
October 01, 2007
Construction of Bacterial Artificial Chromosome (BAC/PAC) Libraries
July 01, 2001

T4 Polynucleotide Kinase

T4 Polynucleotide Kinase from New England Biolabs

Description

New England Biolabs

Citations (1072)

Reviews

Protocols

In Vitro Transcription and Immobilized Template Analysis of Preinitiation Complexes

In Vitro Selection of RNA Aptamers to a Protein Target by Filter Immobilization

Serial Analysis of Gene Expression (SAGE): Experimental Method and Data Analysis

Construction of Bacterial Artificial Chromosome (BAC/PAC) Libraries

T4 Polynucleotide Kinase from New England Biolabs

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