Jess Fully Automated Protein Separation and Immunodetection from ProteinSimple, a Bio-Techne Brand

Product Specs
ItemJess Fully Automated Protein Separation and Immunodetection
CompanyProteinSimple, a Bio-Techne Brand
Price Get Pricing Get Quote for this Product
Catalog Number004-650
Dimensions(WxDxH) 0.3 x 0.57 x 0.36 m (closed)
QuantityEA
ApplicationsProtein quantitation / Protein phosphorylation and post translational modification analysis / Protein analysis from complex sample types like cell and tissue lysates
Operating Temperature18 to 24 °C
Number of Samples13 or 25 samples per run
Sample Volume3 µL/well

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ProteinSimple, a Bio-Techne Brand

3001 Orchard Parkway

San Jose, California 95134

United States

Phone: (888) 607-9692 (U.S. Only)
Phone: (408) 510-5500
Fax: (408) 510-5599

Email: info@proteinsimple.com

Company Profile

Website: https://www.bio-techne.com/brands/proteinsimple

(1) Investigation of Immunostimulatory Effects of Heat-treated Lactiplantibacillus plantarum LM1004 and its Underlying Molecular MechanismsFood Science of Animal ResourcesNovember 1, 2022Won-Young Bae, Woo-Hyun Jung, So Lim Shin, Seulgi Kwon, Minn Sohn, Tae-Rahk Kimaccording to the manufacturer’s guidelines. Protein separation and immunodetection were conducted using JESS, an automated western blotting system (ProteinSimple). For SDS-PAGE, proteins (30 μg) were separated

(2) Retinoids rescue ceruloplasmin secretion and alleviate oxidative stress in Wilsonâ€™s disease-specific hepatocytesHuman Molecular GeneticsOctober 28, 2022Dan Song, Gou Takahashi, Yun-Wen Zheng, Mami Matsuo-Takasaki, Jingyue Li, Miho Takami, Yuri An, Yasuko Hemmi, Natsumi Miharada, Tsuyoshi Fujioka, et al.western blot device (Simple Western, Wes; ProteinSimple). For the detection of total ALB, ATP7B, and Cp, a Jess/Wes 12- to 230-kDa separation module for Wes, 8X25 capillary cartridges (ProteinSimple) and Anti-Rabbit/Goat/Mouse

(3) Oral administration of human carbonic anhydrase I suppresses colitis in a murine inflammatory bowel disease modelScientific reportsOctober 26, 2022Tange K, Yagi S, Takeshita E, Abe M, Yamamoto Y, Tomida H, Kawamura T, Hanayama M, Matsuura B, Ikeda Y, et al.immunoprecipitation. Immunoblot analysis The target protein, recovered from feces by immunoprecipitation, was separated using the Jess system (automated simple western blot system; ProteinSimple, San Jose, CA, United States), with a 12–230

(4) Single cell transcriptomic profiling of a neuron-astrocyte assembloid tauopathy modelNature communicationsOctober 21, 2022Rickner HD, Jiang L, Hong R, O''Neill NK, Mojica CA, Snyder BJ, Zhang L, Shaw D, Medalla M, Wolozin B, et al.mode. Immunoblotting In all, 1 µg total protein lysates were analyzed on the ProteinSimple SimpleWestern Jess platform with the 12–230 kDA microplate kit (ProteinSimple cat#SM-W004) according to the manufacturer’s

(5) Abnormal Expression of Synaptic and Extrasynaptic GABAA Receptor Subunits in the Dystrophin-Deficient mdx MouseInternational Journal of Molecular SciencesOctober 20, 2022Faouzi Zarrouki, SÃ©bastien Goutal, OphÃ©lie Vacca, Luis Garcia, Nicolas Tournier, AurÃ©lie Goyenvalle, Cyrille VaillendSystem Detection of dystrophin expression in the spinal cord was performed using the capillary Western immunoassay Jess system based on the protocol previously described by Beekman et al. [70] and according to the manufacturer’s

(6) Optimization of a Novel Mandelamide-Derived Pyrrolopyrimidine Series of PERK InhibitorsPharmaceuticsOctober 19, 2022Michael E. Stokes, Matthew D. Surman, Veronica Calvo, David Surguladze, An-Hu Li, Jennifer Gasparek, Matthew Betzenhauser, Guangyu Zhu, Hongwen Du, Alan C. Rigby, et al.followed by centrifugation for 10′ at max speed on a benchtop centrifuge. Protein analysis performed using JESS high-throughput protein analysis instrument (Biotechne) using antibodies for phosphoPERK developed internally

(7) Functional Complementation of Anti-Adipogenic Phytonutrients for Obesity Prevention and ManagementNutrientsOctober 16, 2022Urasaki Y, Le TT.buffer, primary and secondary antibodies, and chemiluminescent substrates. The default protocols of the Jess system (ProteinSimple) were used for sized-based protein separation and detection in capillaries. β-Actin

(8) Protein and mRNA Quantification in Small Samples of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes in 96-Well MicroplatesMethods in Molecular BiologyOctober 5, 2022Weizhen Li, Julie L. Han, Emilia Entchevacentrifuge, Fig. 2e. 2.3 Immunoprobing Kit for running an assay by Protein Simple, containing: Jess/Wes 25-capillary cartridge, Fig. 2f.Jess/Wes separation module (12–230 kDa) with a pre-filled microplate

(9) Monoamine Oxidase Inhibitors Prevent Glucose-Dependent Energy Production, Proliferation and Migration of Bladder Carcinoma CellsInternational Journal of Molecular SciencesOctober 4, 2022Jessica Resta, Yohan Santin, Mathieu RoumiguiÃ©, Elodie Riant, Alexandre Lucas, Bettina Couderc, Claudia Binda, Philippe Lluel, Angelo Parini, Jeanne Mialet-Perez(Cell Signaling Technology #9308), protein quantification was performed using a capillary-based "Simple Western” system (Jess, ProteinSimple, bio-techne, Noyal Châtillon sur Seiche, France). Separation and immunoprobing were performed

(10) Treatments targeting autophagy ameliorate the age-related macular degeneration phenotype in mice lacking APOE (apolipoprotein E)AutophagyOctober 1, 2022Vessey KA, Jobling AI, Tran MX, Wang AY, Greferath U, Fletcher EL.Simple western analysis for antibodies with multiple bands, specifically, LC3 were assessed using the JESS system (Protein Simple, Trendbio, Victoria, Australia), which runs small volumes of lysate within a

(11) Proteomic and phosphoproteomic landscapes of acute myeloid leukemiaBloodSeptember 29, 2022Michael H. Kramer, Qiang Zhang, Robert Sprung, Ryan B. Day, Petra Erdmann-Gilmore, Yang Li, Ziheng Xu, Nichole M. Helton, Daniel R. George, Yiling Mi, et al.were harvested 48 hours after nucleofection. Western blotting Western blots were performed using the Jess western blotting system and total protein normalization module and analyzed using Compass software (ProteinSimple).

(12) MicroRNA Associated with the Invasive Phenotype in Clear Cell Renal Cell Carcinoma: Let-7c-5p Inhibits Proliferation, Migration, and Invasion by Targeting Insulin-like Growth Factor 1 ReceptorBiomedicinesSeptember 28, 2022Kalantzakos TJ, Sebel LE, Trussler J, Sullivan TB, Burks EJ, Sarita-Reyes CD, Canes D, Moinzadeh A, Rieger-Christ KM.concentration and volume and loaded in a unique well of a 12–230 kDa separation module on the Simple Western Jess system (ProteinSimple, Santa Clara, CA, USA). IGF1R primary antibody (NBP1-77679, Novus Biologicals

(13) A Composition of Phytonutrients for Glycemic and Weight ManagementNutrientsSeptember 14, 2022Yasuyo Urasaki, Thuc T. Leprotein separation and detection in 24 capillaries were performed using the default protocols of the Jess system (ProteinSimple). β-Actin was used as a loading control. All capillary Western immunoassays were

(14) Methodologies to unlock the molecular expression and cellular structure of ocular lens epithelial cellsFrontiers in Cell and Developmental BiologySeptember 13, 2022Justin Parreno, Grace Emin, Michael P. Vu, Jackson T. Clark, Sandeep Aryal, Shaili D. Patel, Catherine Chengfrom 6–8-week-old mice is 6–10 μg μL and 4–6 μg/μL, respectively. Protein sample preparation for JESS/WES capillary electrophoresis The detection of proteins from pairs of lens epithelial cell samples requires

(15) Thymoquinone Inhibits JAK/STAT and PI3K/Akt/ mTOR Signaling Pathways in MV4-11 and K562 Myeloid Leukemia CellsPharmaceuticals (Basel)September 8, 2022Futoon Abedrabbu Al-Rawashde, Abdullah Saleh Al-wajeeh, Mansoureh Nazari Vishkaei, Hanan Kamel M. Saad, Muhammad Farid Johan, Wan Rohani Wan Taib, Imilia Ismail, Hamid Ali Nagi Al-Jamal(CBB) solution from Nacalai tesque, Inc. (Kyoto, Japan). 4.7. Protein Expression Analysis Using JESS Simple Western Analysis Protein expression was investigated using the capillary-based Protein Simple

(16) Warburg-like metabolic transformation underlies neuronal degeneration in sporadic Alzheimer's diseaseCell metabolismSeptember 6, 2022Traxler L, Herdy JR, Stefanoni D, Eichhorner S, Pelucchi S, Szücs A, Santagostino A, Kim Y, Agarwal RK, Schlachetzki JCM, et al.

(17) FcγRIIB regulates autoantibody responses by limiting marginal zone B cell activationJournal of Clinical InvestigationSeptember 1, 2022Ashley N. Barlev, Susan Malkiel, Izumi Kurata-Sato, Annemarie L. Dorjée, Jolien Suurmond, Betty Diamondice, the cell lysate was collected and stored frozen. Capillary Western blotting was performed with Jess (Protein Simple) according to the manufacturer’s instructions. Briefly, cell lysates from 1 × 105 cells

(18) Evidence of antibodies against SARS‐CoV‐2 in wild mustelids from Brittany (France)Transboundary and Emerging DiseasesSeptember 1, 2022Bernard Davoust, Patrick Guérin, Nicolas Orain, Camille Fligny, Fabien Flirden, Florence Fenollar, Oleg Mediannikov, Sophie Edouard< 60%. 2.2.2 SARS‐CoV‐2 antigen preparation and automated western immunoblotting (AWB) assay The Jess™ Simple Western automated nano‐immunoassay system (ProteinSimple, San Jose, CA, USA, a Bio‐Techne Brand)

(19) CAPG Is Required for Ebola Virus Infection by Controlling Virus Egress from Infected CellsVirusesAugust 28, 2022Mori H, Connell JP, Donahue CJ, Boytz R, Nguyen YTK, Leung DW, LaCount DJ, Davey RA.lysate to a final concentration of 1%, and the mixture was boiled for 10 min. Immunoblotting used a Jess capillary electrophoresis system (ProteinSimple, San Jose, CA, USA) according to the manufacturer’s

(20) Lipid Peroxides Mediated Ferroptosis in Electromagnetic Pulse-Induced Hippocampal Neuronal Damage via Inhibition of GSH/GPX4 AxisInternational Journal of Molecular SciencesAugust 17, 2022Yunfei Lai et al.run using a sample preparation kit (Protein Simple) on the automated capillary Western blot system, JESS System (Protein Simple). Then, the levels of the ferroptosis-related antioxidant GPX4 were analyzed.

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Jess: Your Complete Protein Analysis Solution

Jess Fully Automated Protein Separation and Immunodetection from ProteinSimple, a Bio-Techne Brand

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ProteinSimple, a Bio-Techne Brand

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Jess Fully Automated Protein Separation and Immunodetection from ProteinSimple, a Bio-Techne Brand

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