Studying proteins may not be as hazardous as fishing the high seas, but pursuing proteomics can certainly feel much less gratifying. Hauling in nets full of bounty is sure to bring satisfaction; but you’d probably want to jump overboard after trying to pick out the one tiny minnow swimming in a school of a million halibut. Plunging into the deep, however, will only bring about hypothermia. Here’s a better suggestion—test out some of the latest in accessories and gadgetry. You’re sure to regain the excitement of protein exploration.
Thinking about defining the entire proteome can indeed seem overwhelming. As described in a paper published in a 2005 issue of the Annals of Oncology, genomics is characterizing the letters, words, and sentences of gene sequences—“But we have only merely begun reading the chapter contents (how genes may be transcribed), while the books (the proteins and the metabolites) will keep human mankind reading for many centuries to come,” the authors wrote.
They’re not exaggerating. Unlike the relatively static genome, the proteome encompasses “the collection of all the proteins expressed from the genome in all isoforms, polymorphisms and post-translational modifications,” according to a paper published in a 2004 issue of the Journal of Physiology. In addition to such a dynamic nature, the low abundance presence of the proteome also poses challenges, which can seem daunting but aren’t insurmountable. Optimize each step. That includes boosting expression, maximizing purification, and refining analysis—while improving efficiency.
It’s all possible using the new instruments developed with proteomics in mind. Most take over the time-consuming manual tasks, adding reproducibility and speed to your project. This not only helps to break through bottlenecks, but also releases your time to focus on analysis. Automated devices, many of which use miniaturized formats, allow you to reduce the use of expensive reagents and manpower. For purification, consider systems that perform isoelectric focusing for fractionating protein samples. Or choose from options that carry out gel electrophoresis or liquid chromatography for isolating target proteins. Most systems are designed for easy attachment of additional modules and no-mess loading of consumables.
Integrated proteomics systems with mass spectrometry or other identification technologies not only save time, but also prevent the loss of valuable samples. Purified proteins can immediately enter the next phase. You can find various models with equally varied features. For more complex proteins, you may be interested in systems that digest proteins into peptides prior to mass spectrometry. The features of the installed software should also play into your purchase decision. Be sure the package can perform the types of analyses that you need. You can also find stand-alone software that may offer more.
The products below represent some of the best options on the market. Studying proteins may never be without frustration. But, if you ever decide to jump ship, these lifeboats will steer you in the right direction.
The ZOOM® Benchtop Proteomics™ System is an integrated solution for easy 2-D electrophoresis, in a mini-gel format. This innovative system includes components for solution-phase sample fractionation, first and second dimension gel electrophoresis, a complete line of supporting reagents and stains, and a versatile power supply. The ZOOM® Benchtop Proteomics™ System makes sensitive protein profiling easily accessible to novice as well as experienced proteomic researchers.
Shimadzu Biotech’s new AXIMA-TOF2 is the next generation in MALDI CID MS/MS. It delivers the highest energy collisions (CID with laboratory energy of 20keV) to unlock more information with higher sensitivity, and incorporates novel ion gate technology for high resolution precursor ion selection, simplifying TOF-TOF spectra and data interpretation, even in the presence of complex mixtures. An invaluable system for a multitude of applications, the AXIMA-TOF2 delivers increased flexibility, excellent sensitivity, and unprecedented simplicity.
The BioLogic LP low-pressure chromatography system is ideal for biomolecule purification. It brings high performance, versatility, ease of use, and affordability to low-pressure applications. The compact design maximizes available workspace in the coldroom and on the laboratory bench.
