 Taq Polymerases
Jul 15 '08
Taq polymerase is a DNA polymerase named after the thermophilic bacterium Thermus aquaticus from which it was originally isolated. The thermaphilic bacterium thrives in the hot environments surrounding underwater volcanic vents and consequently, its enzymes are thermostable. The thermostability of these enzymes is the critical feature that allowed the development of PCR, one of the key inventions in molecular biology which has radically changed how molecular research is conducted. This technique is used to amplify DNA utilizing the amazing processivity of the Taq DNA polymerase. When researching which Taq polymerase to purchase for your own experiments, there are a large number of factors to consider and the choice you make will be driven to a large extent by what you wish to accomplish. Factors you might wish to consider include: the yield (i.e. processivity of the Taq), the fidelity or error rate of the Taq, whether you want a Taq which will amplify long sequences of DNA or whether you need to amplify through regions of DNA which are complex or GC rich. These may be some of the more commonly considered factors but the list is by no means exhaustive. For example, Taq is also available with added buffers and/or dye for subsequent gel electrophoresis or in a kit with different buffers for optimization of your PCR reaction. Finally, Taq for hot-start PCR is available to reduce non-specific primer extension. Mulitple cocktails of different Taq enzymes and buffers are available on the market today. Check out the products below, which feature some of the factors mentioned above, to get started on your search for a Taq polymerase that best suits your needs.
Boyd Scott
Senior Research Scientist
Vitae Pharmaceuticals
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| Company |
EPICENTRE Biotechnologies |
MIDSCI |
Promega |
Stratagene |
| Item |
MasterAmp™ Taq DNA Polymerase Kit |
Long Taq Polymerase from BullsEye |
GoTaq® Flexi DNA Polymerase |
Taq2000™ DNA Polymerase |
| Price |
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| Description |
MasterAmp™ Taq DNA Polymerase is a thermostable DNA polymerase derived from Thermus aquaticus. Having optimal activity at temperatures above 70°C, it was the first thermostable enzyme used for PCR and remains the predominant enzyme for that purpose...
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Bullseye Long Taq Polymerase combines Bullseye s high quality Taq DNA Polymerase with a small amount of PR Polymerase, which has a 3 ®5 proofreading exonuclease activity. The Long Taq Enzyme mix is optimised for amplifications of 5-20 kb with a high yield. ...
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GoTaq® Flexi DNA Polymerase(a) offers enhanced amplification over conventional Taq DNA Polymerase. The enzyme is supplied with a tube of 25mM MgCl2, allowing optimization of the magnesium concentration in your reactions. 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer are also provided...
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Taq2000™ DNA polymerase‡ is a highly purified, recombinant Taq DNA polymerase cloned from the thermophilic eubacteria, Thermus aquaticus. Using Taq2000™ DNA polymerase in long PCR amplifications reduces smearing and virtually eliminates unwanted background artifacts...
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| Catalog Number |
Q82100 |
BE270701 |
M8296 |
600195 |
| Quantity |
100 U |
100 Rxns |
2,500u (5 × 500u) |
100 U |
| Buffer |
Includes 10X PCR Buffer, 25 mM MgCl2, and MasterAmp™ 10X PCR Enhancer. |
Storage and Dilution Buffer: Enzyme is supplied in 20 mM Tris-HCl pH 8.3, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween 20® , 0.5% NP40, 50% glycerol. |
5X Green GoTaq® Flexi Buffer, 5X Colorless GoTaq® Flexi Buffer |
10X Taq DNA polymerase buffer |
| Concentration |
5 U/µl |
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5 U/µl |
5 U/µl |
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NOTE: Biocompare disclaims any information on this site. Price information is approximate list price and actual prices may vary.
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