 Cell Counting & Viability Analysis Systems
Apr 30 '08
Cell counting and determination of cell viability are integral parts of successfully culturing mammalian cells. Consequently, cell counts are a daily part of the cell culture scientist’s job. Traditionally, cell counts are performed using a hemocytometer and microscope and viability is determined by the use of Trypan blue exclusion methodology. Both tasks are repetitive, time consuming and subject to operator error. In addition, the use of the Trypan blue exclusion method is also frequently flawed due to staining artifacts which make it difficult for the operator to differentiate between differential Trypan blue uptake. Cell aggregation can also complicate the counting process. Taken together, it is easy to see why variation in manual cell counting is not uncommon. Many of these challenges can be overcome with current automated technologies and indeed, many laboratories have switched to automated means of cell counting. A number of different methodologies are employed in automated systems, such as the traditional Trypan blue exclusion and propidium iodide intercalation of nuclear DNA. Features that need to be considered when researching the purchase of such a system include the type and frequency of calibration that is required for the system, the time required for the analysis, the degree of automation achievable with the system and the amount of maintenance required. Lastly, an important consideration is the quantity of cells required to perform the measurement; this will be particularly important when working with cells where there is a limited supply, such as primary cell lines. Check out the products below to get started on your search for the kit that best suits your needs.
Boyd Scott
Senior Research Scientist
Vitae Pharmaceuticals
The products displayed below were selected for comparison by the author.
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