 Go With The Flow
Apr 17 '08
Flow cytometry systems are fancy tools of the XXI century. The list of flow cytometry applications is very long and constantly expanding. These instruments allow you to count and sort different kinds of tiny particles (like cells, bacteria, worms, embryos and others) suspended in a stream of droplets. Modern flow cytometers permit physical and chemical characterization of thousands of particles in every second, in "real time". When choosing a flow cytometer, remember to decide upon which light source you want to use (and are willing to pay for): mercury or xenon lamps, water-cooled lasers, air-cooled lasers, or diode lasers, as well as how many lasers and fluorescence detectors you need (in general, more is better, but greater sensitivity comes at a price). Another question is whether you want also to obtain digital images of individual cells, which could help you to determine the cellular location of the fluorescent signal. Finally, check whether your new flow cytometer has a friendly (inter)face. These sophisticated machines are more and more popular, so if you decide to “go with the flow” and purchase your own, make sure that you’ll enjoy its full potential.
Andre Pietrzykowski, MD, PhD
Research Assistant Professor
University of Massachusetts Medical School
The products displayed below were selected for comparison by the author.
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Accuri Cytometers, Inc. |
BD Biosciences |
Beckman Coulter |
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C6 Flow Cytometer™ System |
BD FACSCanto™ flow cytometer |
CELL LAB QUANTA* Flow Cytometer |
| Price |
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| Description |
Accuri's C6 Flow Cytometer™ System is a full featured, two laser, six detector analytical flow cytometer system including: , the C6 Flow Cytometer, CFlow™ Software, and a PC for operation and analysis. The C6 Flow Cytometer System provides all the capabilities of the best selling flow cytometers at a fraction of the price...
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BD has been innovating flow cytometry since the release of the first commercial flow cytometer in 1974. That tradition continues with the new BD FACSCanto™ benchtop flow cytometer. Its key features are:- True 6-color capability — Get more information per cell from smaller sample volumes
- Sensitive (<50 MESF PE, <100 MESF FITC*) — Resolves the dimmest...
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The Cell Lab Quanta’s unique combination of Coulter volume and fluorescence provides unsurpassed population resolution capabilities and accurate cell counting. This flow cytometer has multiple excitation wavelengths, including UV, allowing flexible fluorochrome selection for a variety of multi-color applications typically only achievable with larger, more expensive systems...
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| Catalog Number |
SY-01 |
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| Detection |
4 colors standard |
6 color |
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| Excitation |
488nM and 640 nM lasers standard |
Air-cooled, 20 mW solid state 488 nm and 17 mW HeNe 633 nm lasers |
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