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Gel Imaging Systems

Apr 1 '08
Gel imaging systems can be used with Western blots, including multicolor Westerns; arrays; fluorescent DNA and protein gels; and for colony counting. Buying an imaging system can be incredibly complicated and the choices are vast. As with any large piece of equipment, there are a wide variety of prices to match the wide variety of features. My main advice would be to start small. Assess your basic needs and make sure those are met before being talked into a larger, more complicated system that may only interfere and unnecessarily hamper basic functions. The most basic option for a gel system is the ability to detect chemiluminescence. Camera specifications and features are the next piece of the system to research. There are cooled CCD cameras with various megapixels and bits, zoom capabilities and lenses. This can be important when low-light conditions and weak signals are being imaged, especially for use in publications. However, if UV imaging is all that is needed, then a basic camera is more than adequate. Most of the other hardware is pretty standard – imaging cabinet, UV transilluminator, fold down white table, filter wheel and epi illumination. Software, however, can vary widely. If possible, try out the system and its features. The ability to control the machine with the computer, to network, to write on and edit images, and to print are some of the most useful features available. Oftentimes, a small printer is part of the package, which is usually necessary and convenient for documentation. A great imaging system can be incredibly useful in the lab and you will be amazed how many of the added features will come to grow on you. It can be tricky, however, to find that fine balance between too much and not enough; so sit down, review the options, try out a system, and then image away.

Elizabeth Hawkes
Research Associate
University of California, San Francisco

  FluorChem FC2 Imaging System from Alpha Innotech Kodak Image Station 4000MM Pro from Carestream Molecular Imaging formerly Kodak Molecular Imaging Systems MF ChemiBIS 3 2 from DNR Bio Imaging Systems Ltd GelDoc It System from UVP
Company Alpha Innotech Carestream Molecular Imaging (formerly Kodak Molecular Imaging Systems) DNR Bio-Imaging Systems Ltd. UVP
Item FluorChem FC2 Imaging System Kodak Image Station 4000MM Pro MF-ChemiBIS 3.2 GelDoc-It System
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Description The FluorChem FC2's applications include Western blots, SuperArrays, multicolor Westerns, fluorescent DNA and protein gels, and colony counting. The imaging system uses a 1.92 MP cooled CCD camera and includes a high end imaging cabinet, UV transilluminator with dual wavelength 302/365 nm wavelengths, fold down white light table, epi white illumination, optional Epi UV...
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Introducing the New KODAK Image Station 4000MM Pro. This latest addition to the Image Station line brings automation and ease of use to an entirely new level, providing reproducible and quantitative imaging of chemiluminescent, fluorescent, and chromogenic labels in gels, blots, and plates...
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DNR's breakthrough MF-ChemiBIS family of systems is highly advanced image analysis system for 1D and 2D fluorescent, chemiluminescent, and colorimetric applications.

With advanced robotic camera, controlled by the software and motorizes up and down automatically, the MF-ChemiBIS family of systems outperform all other image analysis systems by easier and faster...
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The GelDoc-It Imaging System is designed as the laboratory gel documentation and image analysis workhorse.
  • The compact design includes a benchtop transilluminator with a 25 x 26 cm filter and 302nm UV. Other models are available
  • The GelDoc-It system offers a high resolution CCD camera for quality image capture
  • Simple to set-up, the...
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Catalog Number 92-13778-00 Request Info 750-1-32 Request Info
Lighting UV Transilluminator (ML-26) Fluorescence 10-position selectable multiwavelength xenon epi-illumination, 380 - 780 nm
UV (306 nm) transillumination (optional)
White Light White light epi-illumination and white light transillumination
Trans-UV, Trans-WL, Epi-WL, Epi-UV, Monochromatic LEDs system 25 x 26 cm filter and 302nm UV benchtop transilluminator
Camera 1.92 Megapixel cooled camera with 16 bit A/D, Cooled to -25 degrees celcius absolute and regulated, Low Noise characteristic; applicable for low light applications CCD: 2048 x 2048 pixels 2,184(H) x 1,510(V); 3.2 Mpixel CCD camera high resolution CCD camera
Computer Request Info PC and Mac Options avaiable with FIREWIRE /IEEE 1394 interface Request Info PC-based system
Software AlphaEaseFC Image Anlaysis Software WINDOWS 2000/XP MACINTOSH OS Image capture: GelCapture, lifetime free upgrade
Image analysis: TL-100 for 1D analysis (Nonlinear Dynamics)
Optional 1D analysis software: TL-120, TL-120 DM (Nonlinear Dynamics), Optional: 2D analysis software: PG-200, Prodigy SameSpots or Progenesis SameSpots (Nonlinear Dynamics).
Image Acquisition and Analysis Software is designed as a sophisticated software package for 1D analysis of gels, plates and membranes as well as colony counting. The user friendly software functions feature:
Zoom Lens 28-70mm, F2.8 manual zoom lens 10X zoom, 20 - 200mm, ƒ2.8 Motor controlled, super bright lens.
F-number 0.95 / 1.2.
Motorized F1.2 zoom
Chemiluminescence Compatible Yes Yes Yes No
Information Request Info Request Info Request Info The GelDoc-It and GelDoc-It TS systems may be used the following applications: * Non-Fluorescent & Colorimetric Gels: Coomassie Blue, Silver stain, Autorad film, colony plates,# Fluorescent Gels: EtBr, SYPRO Red, Texas Red, SYPRO Ruby, CY3, RFP, SYPRO Orange, Rhodamine, SYBR Green, EGFP, Fluorescein, Pico Green, Gel Star, SYBR Gold/The Ethidium Bromide interference...
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Monitor Request Info Request Info Request Info Request Info
Printer Request Info Request Info Request Info Thermal Printer( Compact, footprint printer,Supplies 256 gray scale for high resolution, archive-quality thermal film prints,Process low-cost prints in seconds)
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  Alpha Innotech Carestream Molecular Imaging formerly Kodak Molecular Imaging Systems DNR Bio Imaging Systems Ltd UVP

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