 TUNEL Assays That Won’t Keep You In The Dark
Mar 10 '08
So you want to see the death with your own eyes. The flickers of dying, apoptotic cells can be visualized with a Terminal deoxynucleotide transferase dUTP Nick-End Labeling (TUNEL) assay. This assay fluorescently labels ends of fragmented DNA (a biochemical hallmark of apoptotic cells), which than can be detected by flow cytometry or microscopy. There are many TUNEL kits to choose from (same examples are below). In making a purchasing decision, it is important to decide upon the type of detection system you will use and choose a kit accordingly. TUNEL assay kits offer labelings based on bromolated deoxyuridine triphosphate nucleotides (Br-dUTP), fluorescein, biotin or a fluorescently dual-labeled oligonucleotide. Check also whether positive and negative control cells are included in a kit, as well as reagents for counter-staining of the total DNA for quantitation analysis. Be also sure that the TUNEL visualization system is compatible with your fluorescent detection equipment. Once you made you purchase decision you can search for glowing death sparks at the end of the TUNEL.
Andre Pietrzykowski
Instructor
University of Massachusetts
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| Company |
Calbiochem |
Millipore (CHEMICON / Upstate / Linco) |
Molecular Probes (Invitrogen) |
Promega |
| Item |
Apo-BrdU™ Kit |
ApopTag® ISOL Dual Fluorescence Apoptosis Detection Kit (DNase Types I & II) |
APO-BrdU™ TUNEL Assay Kit with Alexa Fluor® 488 anti-BrdU |
DeadEnd™ Fluorometric TUNEL System |
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| Description |
60 Tests. AVOID FREEZE/THAW CYCLES. A two color TUNEL (Terminal deoxynucleotide transferase dUTPNick End Labeling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry and microscopy...
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The ApopTag ISOL Dual Fluorescence Kit utilizes a proprietary double hairpin, dual fluorescently labeled oligonucleotide labeling process to detect and distinguish between typical apoptotic DNA breaks induced by either DNase I or DNase II enzyme activities...
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APO-BrdU™ TUNEL Assay Kit *with Alexa Fluor® 488 anti-BrdU* *60 assays* |
The DeadEnd™ Fluorometric TUNEL System(a) is a classic TUNEL Assay designed for the specific detection and quantitation of apoptotic cells within a cell population. The DeadEnd™ Fluorometric TUNEL System measures nuclear DNA fragmentation, an important biochemical hallmark of apoptosis in many cell types...
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| Catalog Number |
CBA040-1KIT |
APT1000 |
A23210 |
G3250 |
| Quantity |
60 Tests |
25 assays |
60 assays |
60 Assays |
| Size |
1 kit |
25 assays |
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| Applications |
Flow cytometry |
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fluorescence microscopy, flow cytometry |
fluorescence microscopy, flow cytometry |
| Kit Contents |
A two color TUNEL (Terminal deoxynucleotide transferase dUTPNick End Labeling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry and microscopy. This kit labels the 3'-hydroxy termini of apoptotic DNA fragments with bromolated deoxyuridine triphosphate nucleotides (Br-dUTP)...
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| Method |
Fluorescence |
dual fluorescently labeled oligonucleotide |
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| References |
Lockshin, R.A., et al. 2001. Nat. Rev. Mol. Cell Biol. 2, 545.Li, X., et al. 1995. Cell Prolif. 28, 571.Li, X., et al. 1995. Cytometry 20, 172.Goldman, A.S., et al. 1983. Proc. Soc. Exp. Biol. Med. 174, 239. |
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| Assay Time |
4-5 h |
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