Key Considerations In Choosing A Lactate Dehydrogenase Assay Kit

Sep 10 '07
The study of cell proliferation and cell viability requires the accurate quantification of the number of viable cells in a cell culture. Choosing between the multitude of cell viability or cytotoxicity assays available on the market can be a challenging task. One convenient and safe alternative to radioactive [³H]-thymidine and the [⁵¹Cr] release assays is the colorimetric measurement of the activity of lactate dehydrogenase (LDH). LDH is a stable enzyme normally found in the cytosol of all cells, but rapidly releases into the supernatant upon damage of the plasma membrane. This non-radioactive assay eliminates the additional hassle caused by isotope disposal and radiation safety compliance. There are a number of factors to consider during the search for the LDH assay that best suits your needs. One of the most important considerations is sensitivity of detection based on the lowest number of cells that can be quantified. Versatility and flexibility are important if one needs the reagent to be compatible with various culture media and/or liquid handling systems for various detection target applications, including cell proliferation, viability (number of live cells), cytotoxicity (number of dead cells) and apoptosis. Another important consideration is whether the assay is amenable to multiplexing for large numbers of plates. Stability and reliability of the read-out signal must be considered for high-throughput screening applications. If the assay involves fewer steps, this will save time and effort. The cost/assay must also be taken in consideration. Finally, the availability of instruments to detect the signal absorbance, fluorescence, luminescence must also be taken into consideration.

Lorelei Silverman
Postdoctoral Fellow
University of Toronto

Company	BioChain	BioVision	Promega	Roche Applied Science	Takara Bio USA
Item	LDH Cytotoxicity Assay Kit	Cytotoxicity Chromogenic Assay Kit	Cell viability Fluorometric (resorufin) Assay Kit, CytoTox-ONE™	Cytotoxicity Colorimetric Assay Kit, PLUS	Cytotoxicity Chromogenic Assay Kit
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Description	The study of cell proliferation and cell viability requires the accurate quantification of the number of viable cells in a cell culture. Therefore, assays for calculating cell viability are necessary for optimizing cell culture conditions, evaluating cell growth factors and nutrients, discovering novel antibiotics and anti-cancer drugs, evaluating toxic effects of... More...	Convenient and non-radioactive alternative to the [3H]-thymidine and the [51Cr] release assays. Based on measurement of activity of lactate dehydrogenase (LDH) which is a stable enzyme normally found in the cytosol of all cells but rapidly releases into the supernatant upon damage of plasma membrane... More...	The CytoTox-ONE™ Homogeneous Membrane Integrity Assay(a) is a fluorometric method for estimating the number of nonviable cells present in multiwell plates. Cell viability is most often defined based on the integrity of the cell membrane... More...	The Cytotoxicity Detection KitPLUS (LDH) is a fast, sensitive, and simple method to quantitate cytotoxicity/cytolysis based on the measurement of LDH activity released from damaged cells using the 96-well or 384-well plate format... More...	Takara’s LDH Cytotoxicity Detection Kit allows simple measurement of LDH activity released from the of damaged cells into the supernatant. This kit is designed to allow a precise, fast and simple colorimetric assay method to quantitate cytotoxicity/cytolysis based on the measurement of LDH activity released from damaged cells into the supernatant... More...
Catalog Number	K6330400	K311-400	G7891	4744934001	TAK MK401
Quantity	400 assays	400 assays	1,000-4,000 assays	1 kit (2000 tests in 96 wells)	2000 assays
Method	Request Info	Chromogenic	Fluorometric (resorufin)	Colorimetric	Chromogenic
Category	Request Info	Lactate Dehydrogenase (LDH) Cytotoxicity Assays	Lactate Dehydrogenase (LDH) Cytotoxicity Assays	Lactate Dehydrogenase (LDH) Cytotoxicity Assays	Lactate Dehydrogenase (LDH) Cytotoxicity Assays
Detection Target	Request Info	Cytotoxicity	Cell viability	Cytotoxicity	Cytotoxicity
Sensitivity	Request Info	Request Info	Request Info	Less than 100 lysed cells can be detected in a 96-well plate.	High sensitivity: Low cell numbers, such as 0.2 - 2 x 102 cells/well, can be available for detection.
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