 Maximum DNA Yield for Minimum Fuss
Apr 23 '07
Genomic DNA is a valuable source of information in many research projects. Genomic DNA Isolation Kits such as those shown below, are available that make isolating the DNA simple. In general, these kits achieve genomic DNA isolation by breaking down the tissue and lysing the cells, removing cellular material (membranes, proteins and RNA) before extracting the DNA by binding to a column, extracting with organic solvents or and/an alcohol precipitation step. When choosing a kit, there are a number of factors to consider. A good place to start is the source or sources of your genomic DNA. Some kits are able to extract DNA from a wide variety of tissues such as blood, hair follicles, plant, bacterial or animal cells with minor changes in protocol. Others are designed for a particular tissue type, and in some cases species-specific kits are available. Secondly, consider the size of your samples, and the amount of DNA you wish to extract - kits can be restricted to a certain sample size or volume because of the limits of the columns or reagents used. It is worthwhile having a close look at individual protocols as there are a number of different ways to lyse cells, remove proteins and RNA and total processing time can vary. Take note of how the DNA is extracted – some kits use organic solvents which have safety implications and other kits may use an alcohol precipitation or wash steps, which can affect future DNA manipulations if not removed completely. Also take a moment to consider the number of samples you wish to process and frequency of kit use –some kits may be more suitable for managing multiple samples at once. High-throughput specific kits are also available.
Phillipa Campbell
PhD Scholar
University of Queensland, Australia
| |
 |
 |
 |
| Company |
Invitrogen |
BioVision |
Millipore (CHEMICON / Upstate / Linco) |
| Item |
Easy-DNA™ Kit |
Genomic DNA Isolation Kit |
Non-organic DNA Extraction Kit |
| Related Content |
   |
|
 |
| Price |
Request Quote |
Request Quote |
Request Quote |
Request
Information |
 |
|
|
| Description |
The Easy-DNA™ Kit is the fastest easiest way to isolate high-quality high-molecular weight genomic DNA from a wide range of cells and tissue types. Sample sizes can range from a single hair follicle to 1 gram of mammalian tissue...
More... |
The Genomic DNA Isolation Kit provides a simple and convenient procedure for rapid isolation of genomic DNA from mammalian cells and tissue samples with high yield and purity. The novel method requires less than 90 minutes to prepare highly pure genomic DNA...
More... |
DNA Extraction Kit is a simple, non-toxic and efficient way to isolate high molecular weight genomic DNA from a wide variety of mammalian cell sources including: whole blood, body fluids, bone marrow, isolated mononuclear cells, tissue culture cells and solid tissues...
More... |
| Catalog Number |
K180001 |
K281-50 |
S4520 |
| Quantity |
15-200 |
50 isolations |
40 preps |
| Method |
modified cell lysis/DNA precipitation |
Uses a unique formulations of buffers and reagents |
Request Info |
| Starting Material |
wide range of cells and tissue types |
Mammalian cells and tissue samples |
mammalian cells |
| Time |
>1.5hrs |
Less than 90 minutes |
~ 3 hours |
| Yield |
7.5ng-1.5mg |
Request Info |
150 µg |
| Phenol / Chloroform |
No/Yes |
Request Info |
No |
| Starting Amount |
Sample sizes can range from 0.5 µl of blood or a
single hair follicle to 1 gram of mammalian tissue. |
Request Info |
< 2.5 × 107 cells. |
More
Information |
Product Information |
Product Information |
Product Information |
More
Products |
Related Products |
Related Products |
Related Products |
| |
 |
|
 |
NOTE: Biocompare disclaims any information on this site. Price information is approximate list price and actual prices may vary.
 Product Comparison Archive
|
