 Amplify Your Choice
Apr 2 '07
The biggest challenge in choosing a Taq polymerase is the fact that there are so many options and they are all so similar, mostly variations of the Thermus aquaticus enzyme. So how do you choose which product to buy? It’s important to ask yourself how difficult your PCR amplification is predicted to be, how important time and speed are, whether optimization is needed, as well as the cost. The differences often come down to the additional buffers, MgCl2, and enhancing solutions that come with the polymerase. The products below all offer different features, whether it be a special enhancing buffer, hot start modification to reduce non-specific binding, buffer with preloaded dye, optimization flexibility, or the ability to just add your DNA and primers to the master mix and you’re done. If optimization is important, a kit that lets you add your own MgCl2 or offers a special buffer such as Qiagen’s “Q-solution” is essential. However, if the PCR is consistent and reliable, then speed and ease of use would be most important and there are several mixes that just require addition of enzyme, DNA and primers. Ultimately, you’ll want to try the product out for yourself (ask for samples from your vendor rep): start with the simplest polymerase and buffer system and proceed to more complex optimization if needed. And most importantly, once you find something that works – stick to it!
by
Elizabeth Hawkes
Research Associate
University of California, San Francisco
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| Company |
Applied Biosystems |
Invitrogen |
Sigma-Aldrich |
Promega |
| Item |
AmpliTaq Gold® DNA Polymerase |
Taq DNA Polymerase Recombinant |
JumpStart Taq DNA Polymerase |
GoTaq® Flexi DNA Polymerase |
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| Price |
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| Description |
AmpliTaq Gold® DNA Polymerase is a chemically modified form of AmpliTaq® DNA Polymerase. When the chemical moiety is attached to the enzyme, the enzyme is inactive. During set-up and the first ramp of thermal cycling (when the reaction is going through non-optimal annealing temperatures), the enzyme is inactive...
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Taq DNA Polymerase is a thermostable enzyme that can withstand prolonged incubation at temperatures up to 95DegreeC without significant loss of activity. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa...
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Sigma's JumpStart Taq DNA Polymerase is an antibody-inactivated hot-start enzyme designed to minimize non-specific amplification while increasing target yield. Once the reaction temperature reaches 70°C, Taq DNA polymerase activity is restored and the resulting PCR exhibits a higher specificity and yield...
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GoTaq® Flexi DNA Polymerase(a) offers enhanced amplification over conventional Taq DNA Polymerase. The enzyme is supplied with a tube of 25mM MgCl2, allowing optimization of the magnesium concentration in your reactions. 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer are also provided...
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| Catalog Number |
N808-0241 |
10342053 |
D9307 |
M8295 |
| Quantity |
250 U |
100 U |
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500u |
| Buffer |
GeneAmp® 10x PCR Buffer II - 1 x 1.5 mL, 25mM MgCl2 Solution - 1 x 1.5 mL |
storage buffer-20mM Tris HCL(PH 8.0),0.1mMEDTA,1mMDTT,50%(v/v)glycerol,stabilizers-10xPCR buffer 200mM Tris -HCL (PH8.4),500mMKCL |
10× reaction buffer a |
5X Green GoTaq® Flexi Buffer, 5X Colorless GoTaq® Flexi Buffer |
| Concentration |
5 U/µl |
5 U/µl |
2.5 units/ěL |
5 U/µl |
| Form |
recombinant |
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NOTE: Biocompare disclaims any information on this site. Price information is approximate list price and actual prices may vary.
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