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The Right Transfection Conditions

Nov 5 '06
Because each cell line is unique, as is each stretch of nucleic acid, every time you want to transfect a new construct or use a new cell line, you have to spend time optimizing the parameters for transfection. Fortunately, kits now exist which help you in this process. The products listed below contain the reagents and protocols to help you optimize your transfection as quickly and efficiently as possible, sometimes in a single experiment.

  Cell Line Opimization Nucleofector Kit from amaxa biosystems Silencer CellReady siRNA Transfection Optimization Kit from Ambion BLOCK iT Transfection Optimization Kit from Invitrogen
Company amaxa biosystems Ambion Invitrogen
Item Cell Line Opimization Nucleofector® Kit Silencer® CellReady™ siRNA Transfection Optimization Kit BLOCK-iT™ Transfection Optimization Kit
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Description The advanced Cell Line Optimization Nucleofector® Kit is the ideal tool for the transfection of virtually any difficult-to-transfect cell line. It enables you to conveniently and rapidly determine the optimal Nucleofection condition of your specific cell line of interest within one experiment. The kit contains two different Cell Line Nucleofector® Solutions V and L...
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Prior to siRNA library screening, optimal siRNA transfection parameters must be identified and verified for each cell type used. The Silencer CellReady siRNA Transfection Optimization Kit, which was developed specifically for optimizing transfection conditions in 96 well plates, dramatically simplifies this...
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The BLOCK-iT™ Transfection Optimization Kit is designed to optimize RNAi transfection using controls for transfection and viability. Each kit contains a BLOCK-iT™ Fluorescent Oligo, which provides:
  • A strong, easily detectable FITC signal indicating transfection efficiency
  • Chemical modifications for a clearer, more persistent cellular signal than...
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Catalog Number VCO-1001 AM86050 13750047
Quantity 2 x 0.9 ml Cell Line Nucleofector® Solution V and L for 18 reactions) 3 plates + 0.4 ml transfection agent 1kit
Features
  • High Efficiencis (efficient transfection of virtually any difficult-to-transfect cell line)
  • Simple and fast (simple and rapid optimization completed in just a few days)
  • Up-to-date optimization strategy (new program and Cell Line Nucleofector Solution combinations are included)
  • An optimized protocol ia available at the link...
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Method Nucleofection - a unique combination of electrical parameters and cell-type specific solutions Request Info Request Info
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  amaxa biosystems Ambion Invitrogen

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