Find Your Phage

Tuesday April 11, 2006

In peptide display, a peptide is expressed as a fusion with a native phage protein. The strength of the system lies in the fact that when the phage displaying the peptide of interest has been identified, the DNA encoding that peptide resides within the phage. In general, researchers who are working with phage display systems are looking for a peptide which interacts with a specific target protein. Therefore, a critical step in working with any peptide display system is the selection of the phage expressing the peptides which specifically bind to the target protein. This selection process is generally performed via a technique called biopanning. Biopanning is performed by mixing the peptide-displaying phage with the target protein, washing, and then eluting the bound phage. In most cases, the target protein is fixed to a solid support, usually a 96-well plate. However, fixing the target protein to a solid support may inactivate or block the site to which the displayed peptide would react. In this case, the binding should be done in solution. If the bait protein you are working with is particularly sensitive to denaturation or sufficiently small that it is likely to have its binding site obscured when fixed to the well of a plate, you will want to consider a biopanning kit with the option of biopanning in solution. Be warned, however, that biopanning on a solid-support is much simpler so, if possible, use a 96-well plate. Whether you ultimately choose to do your biopanning on a plate or in solution, the kits below will help you find your phage.

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Company	Novagen	New England Biolabs	Spring Bioscience
Item	T7Select® Biopanning Kit	Ph.D.-12 Peptide 12-mer Library Kit	Phage Display cDNA Library Screening Kit
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Catalog Number	70018-3	E8110S	PHDS-101
Description	Kit Components • 25 g Blocking Reagent • 2 x 50 ml 10X TBST Wash Buffer • 10 ml T7 Elution Buffer • 0.2 ml BL21 glycerol stock • 0.2 ml BLT5403 glycerol stock • 0.2 ml BLT5615... more	Kit Includes: Sufficient library for 10 separate biopanning experiments, complexity ~2 x 10^9 independent sequences, -28 gIII Sequencing Primer (100 pmol), for manual DNA sequencing, -96 gIII Sequencing Primer (100 pmol), for automated DNA sequencing, Host E... more	Spring offers a large collection of phage display libraries, human adult tissues, fetal tissuea, cell lines, and mouse tissues. All cDNA libraries are constructed in pHD9 vector. The size of... more
Size	1 kit	1 Kit	2x96 rxns
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