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Biocompare Home > Back > Coming Clean: Kits For Total RNA Isolation

Coming Clean: Kits For Total RNA Isolation

Mar 8 '05
Whether you are working with prokaryotic or eukaryotic cells, total RNA isolation is the first step in most gene expression studies today. Historically, the biggest challenge to molecular scientists has been isolating RNA while simultaneously inactivating the endogenous ribonucleases in order to prevent sample degradation. This typically meant quarantining sections of the lab for RNA work only as well as strict handling practices. Although care must still be taken when working with RNA, the kits available now help provide an extra layer of protection by providing guaranteed RNase-free solutions and consumables. Today’s total RNA isolation kits provide researchers with the tools to obtain high quality and quantity RNA samples from virtually any type of cell. You can compare the products listed below to find the kit that would work best with your specific research needs.

  Agilent Total RNA Isolation Mini Kit from Agilent Technologies PARIS Kit from Ambion GenElute Mammalian Total RNA Miniprep Kit from Sigma Aldrich
Company Agilent Technologies Ambion Sigma-Aldrich
Item Agilent Total RNA Isolation Mini Kit PARIS™ Kit GenElute™ Mammalian Total RNA Miniprep Kit
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Catalog Number 5185-6000 AM1921 RTN10
Description Agilent's Total RNA Isolation Mini Kit removes virtually all genomic DNA using a unique prefiltration column. This results in up to 1000-fold reduction of genomic DNA, compared to typical silica or glass fiber methods. Intact, highly purified RNA prepared by this new kit gives you more confidence in your data for critical applications...
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The PARIS Kit uses a fast, simple procedure based on Ambion's RNAqueous® technology to isolate both RNA and native protein from the same experimental sample. The kit also permits separation of nuclear and cytoplasmic fractions prior to RNA and/or protein isolation...
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Samples are lysed and homogenized in guanidine thiocyanate and 2-mercaptoethanol to release RNA and inactivate RNases. Lysates are spun through a filtration column to remove cellular debris and shear DNA. The filtrate is then applied to a high capacity silica column to bind total RNA, followed by washing and elution...
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Quantity 50 50 purifications 10 purifications
Time Request Info Request Info 30 minutes
Yield Request Info Request Info 150µg
Phenol / Chloroform No Request Info Request Info
Starting Amount 5 x 105 - 106 cells, 2.5 - 30 mg tissue Request Info 107cells or 40 mg of tissue
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  Agilent Technologies Ambion Sigma Aldrich

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