Overview
This is an easy and straightforward protocol that gives efficiencies of 10^6 to 10^7 cfu/mg of plasmid DNA. A growth curve is required for each strain that is prepared. See: Inoue, H. et. al. (1990) Gene, 96: 23-28.
Details
Details of this protocol, High Efficiency FCC Preparation And Tx, are located on a web site other than Biocompare Protocols.