Differential Staining of Live and Dead Embryos using Fluorescein Diacetate

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Protocol Information

Title Differential Staining of Live and Dead Embryos using Fluorescein Diacetate
Source J. Lannett Edwards and P.J. Hansen, Dept. of Animal Sciences, University of Florida

Overview

Procedures obtained from Ann Croy (Guelph, Canada) as a modification of procedures detailed in Transplantation 1971 12:148-151 Takasugi, M. An improved fluorochromatic cytotoxic test. Note that the procedure can be used for staining of all cells and not just embryos or oocytes. Also, ethididium bromide can be replaced with propidium iodide, DAPI or Hoescht 33342

Details

Details of this protocol, Differential Staining of Live and Dead Embryos using Fluorescein Diacetate, are located on a web site other than Biocompare Protocols.

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