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A Method For Ameliorating Autoimmune Disease By Passive Transfer Of IVIg-Primed Leukocytes
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| Protocol Information |
| Title |
A Method For Ameliorating Autoimmune Disease By Passive Transfer Of IVIg-Primed Leukocytes  |
| Source |
Nature Protocols; 10.1038/nprot.2006.153 |
| Overview |
| To determine which cell populations interact with IVIg upstream of the inhibition of phagocytosis, we have developed a method for ‘priming’ specific leukocyte populations with IVIg to study their potential role in ameliorating ITP upon passive transfer, which we describe herein. Using this technique, we found that splenic CD11c+ dendritic cells reacted with IVIg, or several IVIg mimetic regimes, were primed to ameliorate ITP upon transfer to thrombocytopenic mice. We anticipate that this novel methodology could also be used to determine the mechanism of action of IVIg in other autoimmune diseases in addition to ITP. By Andrew R Crow, Vinayakumar Siragam, Davor Brinc, Seng Song, John Freedman and Alan H Lazarus. |
| Details |
Details of this protocol, A Method For Ameliorating Autoimmune Disease By Passive Transfer Of IVIg-Primed Leukocytes, are located on a web site other than Biocompare Protocols.
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