Overview
Many experiments such as transfection of cells and organisms, live imaging and certain biochemical assays, require isolation of live, viable bacteria from insects.
This protocol was developed to isolate Wolbachia from adult Drosophila, but it can be adapted for other insects. In some insects leg removal prior to isolation facilitates hemolymph extrusion 1. This is not necessary for Drosophila. By Horacio M Frydman.
Details
Details of this protocol, Isolation Of Live Bacteria From Adult Insects, are located on a web site other than Biocompare Protocols.