Fluorescent Staining Of Cell Surface Antigens On Viable Cells Using Indirect Immunofluorescence (Subscription Required)

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Protocol Information
Title	Fluorescent Staining Of Cell Surface Antigens On Viable Cells Using Indirect Immunofluorescence (Subscription Required)
Source	CSH Protocols; 2006; doi:10.1101/pdb.prot4440
Overview
This protocol provides a method for analysis of cell surface antigens using indirect immunofluorescence, i.e., when both a primary and secondary antibody are being used to produce fluorescence. After staining, the cells can be analyzed immediately by flow cytometry or fixed and stored for later analysis.
Details
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